Decreased plasma and tissue levels of vitamin C in a rat model of aging: implications for antioxidative defense

Loo, Bernd van der; Bachschmid, Markus; Spitzer, Volker; Brey, Lena; Ullrich, Volker; Lüscher, Thomas F.

doi:10.1016/s0006-291x(03)00360-7

Cited by 58 publications

(28 citation statements)

References 13 publications

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“…4 An age-dependent increase in MDA levels has been reported in rats. 19,51 Our results on fractionated erythrocytes of rats also show increased MDA levels as a function of age. The observation of increase in MDA in lower fraction erythrocytes (old) reflects increased oxidative stress during this period of life and corroborates with the levels of other oxidative stress markers…”

Section: Figsupporting

confidence: 59%

Markers of Oxidative Stress in Senescent Erythrocytes Obtained from Young and Old Age Rats

Kumar

Rizvi

2014

Rejuvenation Research

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The role of oxidative stress during aging is well documented. Evidence is available linking animal life span to the development of oxidative stress. Up to a certain limit of oxidative stress, cells function to counteract the oxidant effects and to restore redox balance by resetting critical homeostatic parameters. Red blood cells (RBCs) offer a very good model to study cellular senescence. In vivo aging of red blood cells is associated with increased cellular density, which corresponds to increased cell age. The present study aims to investigate agedependent oxidative stress in RBC subpopulations obtained after Percoll density gradient centrifugation from young and old rats. We observe an increase in plasma membrane redox system (PMRS) activity ( p < 0.001) and lipid peroxidation ( p < 0.001) between less dense and senescent RBCs in both young and old rats. Our findings provide evidence of a higher level of oxidative stress in senescent erythrocytes, with the effect being more pronounced in old (24-month-old) rats compared to young (4-month-old) rats. The present findings emphasize the role of oxidative stress not only in organismal aging but also in cell senescence.

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Section: Figsupporting

confidence: 59%

Markers of Oxidative Stress in Senescent Erythrocytes Obtained from Young and Old Age Rats

Kumar

Rizvi

2014

Rejuvenation Research

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“…Vitamin C is a well-known antioxidant that has been shown to efficiently scavenge free oxygen radicals, including superoxide, hydrogen peroxide, hypochlorite, hydroxyl, peroxyl, and singlet oxygen. Thus, vitamin C may diminish certain types of lipid peroxidation and may play a major role in preventing oxidative stress in cardiac, hepatic, and renal tissues of rats (Loo et al, 2003). All these results may provide scientific support in understanding the protective effects of melatonin on plasma antioxidants in oxidative damage produced due to Adriamycin toxicity.…”

Section: Discussionmentioning

confidence: 82%

Melatonin increased vitamin C and antioxidant enzyme values in the plasma,heart, liver, and kidney of Adriamycin-treated rats

Karakılçık¹,

Bıtıren²,

Zerin³

et al. 2015

Turk J Biol

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“…Cardiac oxidative stress, as assessed by measuring cardiac malondialdehyde, has previously been found to increase as a function of age (van der Loo et al 2003). Production of superoxide was also enhanced in ischemic areas of rat hearts after experimental induction of myocardial I/R injury (Liu et al 1997).…”

Section: Discussionmentioning

confidence: 92%

Expression and Activity Patterns of Nitric Oxide Synthases and Antioxidant Enzymes Reveal a Substantial Heterogeneity Between Cardiac and Vascular Aging in the Rat

et al. 2005

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We investigated the effects of aging and ischemia-reperfusion (I/R) injury on the expression and activity of nitric oxide (*NO) synthases and superoxide dismutase (SOD) isoforms. To this end we perfused excised hearts from young (6 months old) and old (31-34 months old) rats according to the Langendorff technique. The isolated hearts were, after baseline perfusion for 30 min, either subjected to 20 min of global no-flow ischemia followed by 40 min of reperfusion or were control-perfused (60 min normoxic perfusion). Both MnSOD and Cu,ZnSOD expression remained unchanged with increasing age and remained unaltered by I/R. However, SOD activity decreased from 7.55 +/- 0.1 U/mg protein in young hearts to 5.94 +/- 0.44 in old hearts (P<0.05). Furthermore, I/R led to a further decrease in enzyme activity (to 6.35 +/- 0.41 U/mg protein; P<0.05) in myocardium of young, but not in that of old animals. No changes in myocardial protein-bound 3-nitrotyrosine levels could be detected. Endothelial NOS (eNOS) expression and activity remained unchanged in aged left ventricles, irrespective of I/R injury. This was in steep contrast to peripheral (renal and femoral) arteries obtained from the same animals where a marked age-associated increase of eNOS protein expression could be demonstrated. Inducible NOS expression was undetectable either in the peripheral arteries or in the left ventricle, irrespective of age. In particular when associated with an acute pathology, which is furthermore limited to a certain time frame, changes in the aged myocardium with respect to enzymes crucially involved in maintaining the redox homeostasis, seem to be much less pronounced or even absent compared to the vascular aging process. This may point to heterogeneity in the molecular regulation of the cardiovascular aging process.

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Decreased plasma and tissue levels of vitamin C in a rat model of aging: implications for antioxidative defense

Cited by 58 publications

References 13 publications

Markers of Oxidative Stress in Senescent Erythrocytes Obtained from Young and Old Age Rats

Markers of Oxidative Stress in Senescent Erythrocytes Obtained from Young and Old Age Rats

Melatonin increased vitamin C and antioxidant enzyme values in the plasma,heart, liver, and kidney of Adriamycin-treated rats

Expression and Activity Patterns of Nitric Oxide Synthases and Antioxidant Enzymes Reveal a Substantial Heterogeneity Between Cardiac and Vascular Aging in the Rat

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