Deep eutectic solvents used as extraction solvent for the determination of flavonoids from <i>Camellia oleifera</i> flowers by high‐performance liquid chromatography

Ma, Yue; Liu, Menghan; Tan, Tao; Yan, Aiping; Guo, Lingling; Jiang, Kun; Tan, Chen; Wan, Yiqun

doi:10.1002/pca.2777

Cited by 41 publications

(18 citation statements)

References 39 publications

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“…The synthesis of natural deep eutectic solvent (NADES) was carried out following the procedure developed by Ma et al. [29] in which choline chloride (ChCl) and acetic acid were mixed at a molar ratio of 1:2. The synthesized batches were prepared and used for 1 week in which NADES was found to be stable.…”

Section: Methodsmentioning

confidence: 99%

Nano‐liquid chromatography combined with a sustainable microextraction based on natural deep eutectic solvents for analysis of phthalate esters

et al. 2020

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The separation of 11 phthalic acid ester (PAEs) was carried out by nano-liquid chromatography coupled to ultraviolet and MS detection. Preliminary experiments were achieved in order to select suitable stationary phases and chromatographic conditions. The baseline separation was obtained, for all compounds, with an XBridge TM C 18 column in less than 15 min, working in step gradient mode. The sensitivity of the method was improved by on-column focusing. PAEs were extracted from alcoholic and nonalcoholic beverages using vortex-assisted emulsification dispersive liquid-liquid microextration and natural deep eutectic solvents. The whole method was validated in terms of linearity, sensitivity, precision, recovery, and repeatability. Combination of both off-line sample preparation preconcentration and large injection volume led to obtain LOQs in the range 5-47 ng/mL. The developed nano-LC-UV method was extended to MS detection to confirm the presence of PAEs in some beverages commercialized in different types of packaging.

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Section: Methodsmentioning

confidence: 99%

Nano‐liquid chromatography combined with a sustainable microextraction based on natural deep eutectic solvents for analysis of phthalate esters

et al. 2020

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“…The above extraction investigations showcased that the tailor-made ternary DES, ChCl-Ma-Ur (2:1:2), was selected as the best ternary DES for extraction of phenolic acids from AL. In order to obtain the optimal extraction efficiency for phenolic acids from AL, several numerical variables that could affect the extraction efficiencies were optimized by RSM, a valuable statistical technique to determine the optimal values of the independent variables and to enable the user to effectively investigate the effects of multiple factors [28,29]. Similar to previous studies [30], three variables of extraction time, liquid–solid ratios, and water content in DESs were evaluated using Box–Behnken design (BBD).…”

Section: Resultsmentioning

confidence: 99%

Green Extraction of Phenolic Acids from Artemisia argyi Leaves by Tailor-Made Ternary Deep Eutectic Solvents

Duan

Zhang

et al. 2019

Molecules

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The Artemisia argyi leaf (AL) has been used as a traditional medicine and food supplement in China and other Asian countries for hundreds of years. Phytochemical studies disclosed that AL contains various bioactive constituents. Among bioactive constituents, phenolic acids have been recognized as the main active compounds in AL. To the best of our knowledge, no research has been focused on extraction method for the bioactive phenolic acids from AL. Nowadays, deep eutectic solvents (DESs) are emerging as a new type of green and sustainable solvent for efficient extraction of bioactive compounds from natural products. In the present study, an environmentally friendly extraction method based on DESs was established to extract bioactive phenolic acids from ALs. Diverse tailor-made solvents, including binary and ternary DESs, were explored for simultaneous extraction of four phenolic acids (3-caffeoylquinic acid, 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, and 4,5-di-O-caffeoylquinic acid) from AL. The results indicated that the ternary DES composed of a 2:1:2 molar ratio of choline chloride, malic acid, and urea showed enhanced extraction yields for phenolic acids compared with conventional organic solvents and other DESs. Subsequently, the extraction parameters for the four phenolic acids by selected tailor-made DESs, including liquid–solid ratios, water content (%) in the DESs, and extraction time, were optimized using response surface methodology and the optimal extraction conditions were: extraction time, 23.5 min; liquid–solid ratio, 57.5 mL/g (mL of DES/g dry weight of plant material); water content, 54%. The research indicated that DESs were efficient and sustainable green extraction solvents for extraction of bioactive phenolic acids from natural products. Compared to the conventional organic solvents, the DESs have a great potential as possible alternatives to those organic solvents in health-related areas such as food and pharmaceuticals.

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“…It has been reported ( 17 ) that miRNA-222-3p and miRNA-151a-5p are highly expressed in patients with papillary thyroid carcinoma, tumor infiltration, medullary carcinoma, tumor diameter >4 cm and distant metastatic tumors; as cancer-causing molecules, they can promote cell proliferation by inhibiting a tumor suppressor gene. According to a previous study ( 18 ), miR-138 overexpression is a negative regulator of mesangial cell cytoprotective factor SIRT1, which inhibits nitric oxide production through endothelial nitric oxide synthase, thus negative regulation of miR-138 could inhibit the fibrosis and inflammatory response during glomerular mesangial damage, suggesting that negative regulation of miR-138 could inhibit thyroid disease. Moreover, the expression of miR-375 in gastric cancer tissues is significantly downregulated, probably due to the negative regulation of the transcription factor Snail, which is upregulated in gastric cancer, suggesting that miR-375 may be involved in the development of gastric cancer ( 19 ).…”

Section: Discussionmentioning

confidence: 95%

Added value of circulating miRNA expression profiling to sonographic TI‑RADS classification in the diagnosis of thyroid nodules

Mao

Zhang

et al. 2020

Exp Ther Med

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Potential use of sonographic TI-RADS classification combined with circulating miRNA expression profiling in the diagnosis of thyroid nodules was explored. Retrospective analysis was performed on clinical data of 121 patients with thyroid nodules. The biopsy specimens of patients obtained through ultrasound-guided aspiration and blood specimens were evaluated in Zhengzhou Central Hospital Affiliated to Zhengzhou University from June 2018 to June 2019. In addition, the blood specimen test results of 121 healthy volunteers (control group) who underwent physical examination were retrospectively analyzed. Results of sonographic TI-RADS classification and circulating miRNA expression profiling were compared with the pathological results. Of the 212 nodules, 2 fell into TI-RADS category 2 and were diagnosed as benign. Malignant nodules accounted for 4.35, 37.14, 84.78, 93.33 and 96.77% of those nodules that fell into TI-RADS categories 3, 4a, 4b, 4c and 5, respectively. Of the 121 patients, 92.55% had with nodular goiter, 3.31% had inflammatory nodules, 2.48% toxic nodular goiter, 0.83% thyroid cysts and 0.83% thyroid tumors. A nodule that fell into a higher TI-RADS classification category had a higher risk of malignancy. The expression levels of miRNA146b, miRNA187, miRNA375, miRNA-222-3p and miRNA-151a-5p were higher, while the level of miRNA138 was lower, in patients with either benign or malignant thyroid nodules compaed to those in the control group. The expression levels of miRNA146b, miRNA187, miRNA375, miRNA-222-3p and miRNA-151a-5p were higher, while the level of miRNA138 was lower, in patients with malignant thyroid nodules than those in patients with benign thyroid nodule (P<0.05). The AUC of the combined diagnostic method was 0.973, which was significantly different from the AUCs of the individual diagnostic method (P<0.05). In conclusion, sonographic TI-RADS classification combined with circulating miRNA expression profiling can improve the diagnosis of thyroid nodules.

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Deep eutectic solvents used as extraction solvent for the determination of flavonoids from Camellia oleifera flowers by high‐performance liquid chromatography

Abstract: DESs could be promising solvents for efficiently and selectively extracting bioactive compounds from plant materials, and the analytical method for flavonoids of C. oleifera flowers could provide reference value for its application and be used in other plant resources.

Cited by 41 publications

References 39 publications

Nano‐liquid chromatography combined with a sustainable microextraction based on natural deep eutectic solvents for analysis of phthalate esters

Nano‐liquid chromatography combined with a sustainable microextraction based on natural deep eutectic solvents for analysis of phthalate esters

Green Extraction of Phenolic Acids from Artemisia argyi Leaves by Tailor-Made Ternary Deep Eutectic Solvents

Added value of circulating miRNA expression profiling to sonographic TI‑RADS classification in the diagnosis of thyroid nodules

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