Deep sequencing analysis of microRNAs in bovine sperm

Du, Yue; Wang, Xiangguo; Wang, Bo; Chen, Wuju; He, Rongjun; Zhang, Lei; Xing, Xupeng; Su, Jianmin; Wang, Yongsheng; Zhang, Yong

doi:10.1002/mrd.22426

Cited by 39 publications

(33 citation statements)

References 50 publications

(55 reference statements)

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“…Several groups have previously investigated the miRNA contents in sperm, for a variety of organisms such as mouse [50, 64], human [2, 4], bull [58, 65–67], and pig [68]. Despite this cache of sperm miRNA expression information, cross-species comparisons using these existing data would be, as discussed previously, intrinsically unreliable because of differences in methodology [20, 21, 23–27].…”

Section: Resultsmentioning

confidence: 99%

SpermBase: A Database for Sperm-Borne RNA Contents

Schuster

Tang

Xie

et al. 2016

Biology of Reproduction

109

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Since their discovery approximately three decades ago, sperm-borne RNAs, both large/small and coding/noncoding, have been reported in multiple organisms, and some have been implicated in spermatogenesis, early development, and epigenetic inheritance. Despite these advances, isolation, quantification, and annotation of sperm-borne RNAs remain nontrivial. The yields and subspecies of sperm-borne RNAs isolated from sperm can vary drastically depending on the methods used, and no cross-species analyses of sperm RNA contents have ever been conducted using a standardized sperm RNA isolation protocol. To address these issues, we developed a simple RNA isolation method that is applicable to sperm of various species, thus allowing for reliable interspecies comparisons. Based on RNASeq analyses, we established SpermBase (www.spermbase.org), a database dedicated to sperm-borne RNA profiling of multiple species. Currently, SpermBase contains large and small RNA expression data for mouse, rat, rabbit, and human total sperm and sperm heads. By analyzing large and small RNAs for conserved features, we found that many sperm-borne RNA species were conserved across all four species analyzed, and among the conserved small RNAs, sperm-borne tRNA-derived small noncoding RNAs and miRNAs can target a large number of genes known to be critical for early development.

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Section: Resultsmentioning

confidence: 99%

SpermBase: A Database for Sperm-Borne RNA Contents

Schuster

Tang

Xie

et al. 2016

Biology of Reproduction

109

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“…As a result, two small-RNA libraries, corresponding to the conditions of chilling stress and control, were constructed. Then we performed the single-end sequencing on an Illumina Hiseq2500 at the LC-BIO (Hangzhou, China) following a recommended protocol49.…”

Section: Methodsmentioning

confidence: 99%

Identification of chilling-responsive microRNAs and their targets in vegetable soybean (Glycine max L.)

Liu

Mao

et al. 2016

Sci Rep

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Chilling stress is a major factor limiting the yield and quality of vegetable soybean (Glycine max L.) on a global scale. In the present study, systematic identification and functional analysis of miRNAs under chilling stress were carried out to clarify the molecular mechanism of chilling resistance. Two independent small RNA libraries from leaves of soybean were constructed and sequenced with the high-throughput Illumina Solexa system. A total of 434 known miRNAs and 3 novel miRNAs were identified. Thirty-five miRNAs were verified by qRT-PCR analysis. Furthermore, their gene targets were identified via high-throughput degradome sequencing. A total of 898 transcripts were targeted by 54 miRNA families attributed to five categories. More importantly, we identified 51 miRNAs differentially expressed between chilling stress and control conditions. The targets of these miRNAs were enriched in oxidation-reduction, signal transduction, and metabolic process functional categories. Our qRT-PCR analysis confirmed a negative relationship among the miRNAs and their targets under chilling stress. Our work thus provides comprehensive molecular evidence supporting the involvement of miRNAs in chilling-stress responses in vegetable soybean.

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“…The advancements in sequencing technology have enabled researchers to explore, in depth, the microRNA profile of many cell types [11], including sperm, where a plethora of novel sperm-specific miRNAs have been described [12,13]. Increased miRNA levels at the pachytene spermatocyte stage and the post-meiotic stage during spermatid development together with specific miRNAs expression in mouse testis suggesting that miRNAs have a role in regulating spermatogenesis [14][15][16][17] Nixon et al has recently provided novel data for the post-testicular modification of sperm miRNA profile under normal physiological conditions.…”

Section: Sperm-specific Mirnas and Male Infertilitymentioning

confidence: 99%

Sperm-Specific MicroRNAs - Their Role and Function

Al-Gazi¹,

Carroll²

2016

GGS

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The sperm cell is highly specialised, with the sole purpose to fertilize an oocyte. Sperm assumes a simplistic structure, consisting of a head, which is mostly void of cytoplasm, a nucleus packed with DNA tightly wound around protamines; a midpiece housing the mitochondria necessary to fuel its locomotion, and a tail or principle piece that whips in motion to move the sperm towards the oocyte. However, despite the lack of cytoplasmic space, increasing studies in sperm biology have revealed that the sperm cell contains a myriad of molecular constituents that may play a role post-fertilization, influencing the oocyte and embryo development (figure 1). One such group of molecules are the small non-coding RNAs, which include the microRNAs (miRNAs). microRNAs miRNAs are small non-coding single-strand RNA molecules with sizes ranging from 17-26 nucleotides. The first miRNA to be discovered was lin-4 in the nematode, Caenorhabditis elegans. Lin-4 was detected with conserved complementary site on lin-14 and subsequently affected the larvae developmental timing by targeting mRNA and inhibiting its function [1]. Since then, miRNAs have emerged to play key roles in many biological functions through binding to 3'UTR (Untranslated Region) of specific mRNAs leading to either mRNA degradation or protein translation inhibition [2]. These miRNAs are involved in regulating cell functions including gene expression during development, differentiation, cell proliferation and apoptosis [3,4].In addition to having an influence in normal biological and developmental processes, miRNAs have been found to be involved in many pathogenic processes including Cardiovascular Diseases (CVD), cancer, inflammatory or autoimmune diseases, metabolic disorders and neurodegenerative disorders [5]. miRNA biogenesis miRNAs are encoded in the genome and transcribed by RNA polymerase II to form long primary miRNA transcripts (pri-miRNAs) that contain a cap structure at the 5' end and are poly-adenylated at the 3' end.The primary miRNAs are subsequently processed in the nucleus by Drosha to form shorter stem loop structures called pre curser-miRNAs or pre-miRNA (figure 2).These pre-miRNAs are then HSOA Journal of Genetics & Genomic Sciences Mini ReviewAbstract microRNAs (miRNAs) are small non-coding single-stranded molecules (containing about 22 nucleotides) that are widely found in many organisms, as varied as micro-organisms, plants, invertebrates and vertebrates. These miRNAs function in RNA silencing and post-transcriptional regulation of gene expression. miRNAs have been found to be involved in a number of biological and pathological processes, including sperm biology and infertility. This review will describe and discuss the role of miRNAs in terms of their role in sperm function, male infertility and gene regulation. a) The fertilising sperm introduces more than the paternal genome, the oocyte-activating factor (PLCζ), proteins from post-acrosomal, and perinuclear thecal proteins and centrioles; b) Sperm also contain a complex population of RNA, ...

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Deep sequencing analysis of microRNAs in bovine sperm

Cited by 39 publications

References 50 publications

SpermBase: A Database for Sperm-Borne RNA Contents

SpermBase: A Database for Sperm-Borne RNA Contents

Identification of chilling-responsive microRNAs and their targets in vegetable soybean (Glycine max L.)

Sperm-Specific MicroRNAs - Their Role and Function

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