Deep sequencing identification of miRNAs in pigeon ovaries illuminated with monochromatic light

Abstract

BackgroundThe use of light of different wavelengths has grown popular in the poultry industry. An optimum wavelength is believed to improve pigeon egg production, but little is known about the role of microRNAs (miRNAs) in the effects of monochromatic light on ovarian pigeon function. Herein, we harvested ovaries from pigeons reared under monochromatic light of different wavelength and performed deep sequencing on various tissues using an Illumina Solexa high-throughput instrument.ResultsWe obtained 66,148,548, 67,873,805, and 71,661,771 clean reads from ovaries of pigeons reared under red light (RL), blue light (BL), and white light (WL), respectively. We identified 1917 known miRNAs in nine libraries, of which 524 were novel. Three and five differentially expressed miRNAs were identified in BL vs. WL and RL vs. WL groups, respectively. Quantitative reverse transcription PCR was used to validate differentially expressed miRNAs (miR-200, miR-122, and miR-205b). In addition, 5824 target genes were annotated as differentially expressed miRNAs, most of which are involved in reproductive pathways including oestrogen signalling, cell cycle, and oocyte maturation. Notably, ovarian miR-205b expression was significantly negatively correlated with its target 11β-hydroxysteroid dehydrogenase type 1 (HSD11B1).ConclusionsmiRNA–mRNA network analysis suggests that miR-205b targeting of HSD11B1 plays a key role in the effects of monochromatic light on pigeon egg production. These findings indicate that monochromatic light shortens the oviposition interval of pigeons, which may be useful for egg production and pigeon breeding.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4831-6) contains supplementary material, which is available to authorized users.

Characterization and Comparative Analysis of Whole-Transcriptome Sequencing in High- and Low-Fecundity Chongming White Goat Ovaries during the Estrus Phase

“…This tendency showed the same trend as that seen in our results. Similarly, Wang et al ( 2018) also reported miR-122 to be a candidate miRNA involved in the effects of monochromatic light on pigeon reproduction [40]. Therefore, the upregulation of chi-miR-122 may be a useful strategy to increase ovulation rates in goats.…”

Identification and Profiling of MicroRNAs During Embryogenesis in the Red Claw Crayfish Cherax quadricarinatus

“…In the present study, three embryonic developmental stages of C. quadricarinatus, including the eye pigment forming stage, prepare-hatching stage, and larva, were selected by small RNA-Seq technology to identify miRNAs and acquire their expression profiles. In the previous reports, transcriptome libraries have been successfully used to search for novel miRNAs and predict target genes in some organisms (Niu et al, 2014;Wang et al, 2018). Although an annotated genome was lacking in C. quadricarinatus to identify miRNAs, the transcriptome of embryogenesis contributed to miRNAs recognition in this study.…”

Identification of miRNA Associated with Trichomonas gallinae Resistance in Pigeon (Columba livia)

“…By studying the pectoral muscle miRNAs of pigeons at different age stages, 89 miRNAs and pathways of MAPK and TGF-β were found to be related [17]. MiR-205b was thought to play a key role in monochromatic light regulation of pigeon egg production [20].…”

“…In this study, the miRNA expression profile of the oral mucosa infected by T. gallinae of pigeons was designed to analyze the potential genes and related miRNAs. Previous studies in pigeon miRNAs have been focused on crop tissues [4], pectoral muscle and ovaries [20]. In the study of crop tissues, miR-20b-5p, miR-146b-5p, miR-21-5p and miR-26b-5p were found to regulate lactation [4].…”