Deep sequencing of the scallop Chlamys farreri transcriptome response to tetrabromobisphenol A (TBBPA) stress

Hu, Fengxiao; Pan, Luqing; Cai, Yuefeng; Liu, Tong; Qian, Jin

doi:10.1016/j.margen.2014.09.004

Cited by 29 publications

(10 citation statements)

References 79 publications

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“…Our results clearly showed that OA provoked the differential expression of CYP1A5 and CYP3A24 , which were downregulated, whereas CYP3A4 and CYP3A80 were upregulated. This is consistent with a previous study that reported OA-exposure-induced expression of CYP450 mussel gills, which suggests that CYP450 participates in the process of OA elimination [ 3 ]. Guo et al also [ 41 ] reported that human recombinant cytochrome CYP3A4 could eliminate OA by generating oxidized products.…”

Section: Discussionsupporting

confidence: 93%

“…Highly conserved heat shock proteins (HSPs), including HSP60 , HSP70 , and HSP90 , could be synthesized or secreted rapidly by cells as soon as they experience stressed [ 3 ]. Therefore, HSPs have been widely considered as effective biomarkers of exogenous stimuli or as biomonitoring tools to identify the effects of environmental pollution in aquatic animals, including bivalves [ 3 ]. Our present investigation showed that the relative expression of HSP70 , which was validated by qPCR, was strongly increased in the gills of bay scallop up to 48 h exposure to OA.…”

Section: Discussionmentioning

confidence: 99%

“…The genes encoding detoxification enzymes play crucial roles in bivalves after being stimulated by a variety of exogenous stimuli, including drugs, toxicants, and chemical carcinogens [ 3 ]. Among the DEGs detected in the present study, certain detoxification-related genes were identified.…”

Section: Discussionmentioning

confidence: 99%

“…Among the DEGs detected in the present study, certain detoxification-related genes were identified. The cytochrome P450 ( CYP450 ) family is an essential family of enzymes related to the primary or phase I metabolism of xenobiotics, including pesticides and toxins [ 3 , 39 ]. Many exogenous stimuli may impact the metabolism, and then activate or suppress the activity of CYP450 to clean exogenous stimuli [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

“…Owing to their filter-feeding and sessile habits, worldwide distribution, and diversity of aquatic environments, bivalves are widely used as marine pollution bioindicators [ 2 ]. Scallop fisheries are mainly distributed along coastal areas of Japan, Korea, and North China [ 3 ]. In addition to their economic value, bivalves have always been studied as model species in toxicological investigation and as sentinel species in environmental monitoring programmes [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

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Detoxification- and Immune-Related Transcriptomic Analysis of Gills from Bay Scallops (Argopecten irradians) in Response to Algal Toxin Okadaic Acid

Cheng

Giri

Jun

et al. 2018

Toxins

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To reveal the molecular mechanisms triggered by okadaic acid (OA)-exposure in the detoxification and immune system of bay scallops, we studied differentially-expressed genes (DEGs) and the transcriptomic profile in bay scallop gill tissue after 48 h exposure to 500 nM of OA using the Illumina HiSeq 4000 deep-sequencing platform. De novo assembly of paired-end reads yielded 55,876 unigenes, of which 3204 and 2620 genes were found to be significantly up- or down-regulated, respectively. Gene ontology classification and enrichment analysis of the DEGs detected in bay scallops exposed to OA revealed four ontologies with particularly high functional enrichment, which were ‘cellular process’ (cellular component), ‘metabolic process’ (biological process), ‘immune system process’ (biological process), and ‘catalytic process’ (molecular function). The DEGs revealed that cyclic AMP-responsive element-binding proteins, acid phosphatase, toll-like receptors, nuclear erythroid 2-related factor, and the NADPH2 quinone reductase-related gene were upregulated. In contrast, the expression of some genes related to glutathione S-transferase 1, C-type lectin, complement C1q tumor necrosis factor-related protein, Superoxide dismutase 2 and fibrinogen C domain-containing protein, decreased. The outcomes of this study will be a valuable resource for the study of gene expression induced by marine toxins, and will help understanding of the molecular mechanisms underlying the scallops’ response to OA exposure.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%