Deep sympatric mt<scp>DNA</scp> divergence in the autumnal moth (<i>Epirrita autumnata</i>)

Kvie, Kjersti S.; Hogner, Silje; Aarvik, Leif; Lifjeld, Jan T.; Johnsen, Arild

doi:10.1002/ece3.434

Cited by 28 publications

(28 citation statements)

References 95 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such cases are best resolved through multi-loci analysis (e.g., a nuclear loci) [49], [50] of specimens from geographic settings where the component lineages are sympatric. If an exhaustive examination of nuclear markers shows no differentiation between lineages, the variation at COI likely reflects deep intraspecific divergence, such as that reported in European populations of the geometrid Epirrita autumnata [51]. The factor(s) responsible for divergence can then be analyzed; it may reflect selective sweeps driven by Wolbachia [52] or secondary contact between lineages formerly isolated in different glacial refugia.…”

Section: Discussionmentioning

confidence: 98%

A Transcontinental Challenge — A Test of DNA Barcode Performance for 1,541 Species of Canadian Noctuoidea (Lepidoptera)

et al. 2014

View full text Add to dashboard Cite

This study provides a first, comprehensive, diagnostic use of DNA barcodes for the Canadian fauna of noctuoids or “owlet” moths (Lepidoptera: Noctuoidea) based on vouchered records for 1,541 species (99.1% species coverage), and more than 30,000 sequences. When viewed from a Canada-wide perspective, DNA barcodes unambiguously discriminate 90% of the noctuoid species recognized through prior taxonomic study, and resolution reaches 95.6% when considered at a provincial scale. Barcode sharing is concentrated in certain lineages with 54% of the cases involving 1.8% of the genera. Deep intraspecific divergence exists in 7.7% of the species, but further studies are required to clarify whether these cases reflect an overlooked species complex or phylogeographic variation in a single species. Non-native species possess higher Nearest-Neighbour (NN) distances than native taxa, whereas generalist feeders have lower NN distances than those with more specialized feeding habits. We found high concordance between taxonomic names and sequence clusters delineated by the Barcode Index Number (BIN) system with 1,082 species (70%) assigned to a unique BIN. The cases of discordance involve both BIN mergers and BIN splits with 38 species falling into both categories, most likely reflecting bidirectional introgression. One fifth of the species are involved in a BIN merger reflecting the presence of 158 species sharing their barcode sequence with at least one other taxon, and 189 species with low, but diagnostic COI divergence. A very few cases (13) involved species whose members fell into both categories. Most of the remaining 140 species show a split into two or three BINs per species, while Virbia ferruginosa was divided into 16. The overall results confirm that DNA barcodes are effective for the identification of Canadian noctuoids. This study also affirms that BINs are a strong proxy for species, providing a pathway for a rapid, accurate estimation of animal diversity.

show abstract

Section: Discussionmentioning

confidence: 98%

A Transcontinental Challenge — A Test of DNA Barcode Performance for 1,541 Species of Canadian Noctuoidea (Lepidoptera)

et al. 2014

View full text Add to dashboard Cite

show abstract

“…biased variation stemming from infection with maternally transmitted parasites (Kvie et al . )]. These cases have the potential to substantially shift key barcode metrics (mean intraspecific variation, mean NN distance) and overall species diversity.…”

Section: Discussionmentioning

confidence: 99%

Untangling taxonomy: a DNA barcode reference library for Canadian spiders

Blagoev

deWaard

Ratnasingham

et al. 2015

Molecular Ecology Resources

108

View full text Add to dashboard Cite

Approximately 1460 species of spiders have been reported from Canada, 3% of the global fauna. This study provides a DNA barcode reference library for 1018 of these species based upon the analysis of more than 30 000 specimens. The sequence results show a clear barcode gap in most cases with a mean intraspecific divergence of 0.78% vs. a minimum nearest-neighbour (NN) distance averaging 7.85%. The sequences were assigned to 1359 Barcode index numbers (BINs) with 1344 of these BINs composed of specimens belonging to a single currently recognized species. There was a perfect correspondence between BIN membership and a known species in 795 cases, while another 197 species were assigned to two or more BINs (556 in total). A few other species (26) were involved in BIN merges or in a combination of merges and splits. There was only a weak relationship between the number of specimens analysed for a species and its BIN count. However, three species were clear outliers with their specimens being placed in 11-22 BINs. Although all BIN splits need further study to clarify the taxonomic status of the entities involved, DNA barcodes discriminated 98% of the 1018 species. The present survey conservatively revealed 16 species new to science, 52 species new to Canada and major range extensions for 426 species. However, if most BIN splits detected in this study reflect cryptic taxa, the true species count for Canadian spiders could be 30-50% higher than currently recognized.

show abstract

“…The sequence size homogenization among individuals, in the case of D. saccharalis and D. flavipennella by breeding, could be due to endogamy that is present in breeding. There was also no report about significant ITS 2 size differences in some lepidopteran species: Chilo sp, Cnaphalocrocis medinalis, Ostrinia sp, Spodoptera spp, Polyommatus spp, and species from the families Geometridae, Lasiocampidae, Pieridae, Tortricidae, and Zygaenidae (Niehuis et al, 2006;Barr et al, 2009;Wiemers et al, 2009;Dincă et al, 2011;Margam et al, 2011;Wan et al, 2011a;Kvie et al, 2012). In other groups as Diptera, ten species belonging to genus Anopheles, from different geographic regions, did not also show intraspecific differences in nucleotide composition and sequence length.…”

Section: Discussionmentioning

confidence: 93%

“…The ITS 2 region size is specific to each species in the family Crambidae, and this also happens in other families as Tortricidae, Noctuidae, Geometridae, and Lycaenidae (Barr et al, 2009;Wiemers et al, 2009;Wan et al, 2011a;Kvie et al, 2012).…”

Section: Discussionmentioning

confidence: 97%

Research Article ITS2 as a molecular marker for the identification of Diatraea saccharalis and D. flavipennella and possible infection with Cotesia spp.

Regueira-Neto

Barros²,

Morais³

et al. 2017

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. In Brazil, the species Diatraea flavipennella and D. saccharalis play an important role in the sugar and alcohol agribusiness by causing many damages in sugarcane fields. The egg, larva, pupa, and adult stages are very morphologically similar between these species, and the identification can be confused. The internal transcribed spacer 2 (ITS 2) from ribosomal DNA has important features as evolutionary divergence. It is a good marker for species identification, participates in the rDNA processing, and has been applied in phylogenetic and population studies. This study aimed to make available a molecular marker to assist on the identification method of pests' species of Diatraea and to identify possible traces of Cotesia in the resistant host. The DNA was extracted from the egg, larva, and adult samples. PCR amplicons were purified and sequenced. The sequences were analyzed in MEGA 5.01. The ITS 2 length was 410 bp in D. flavipennella and 448 bp in D. saccharalis. The GC content was similar between the species. Three microsatellite loci were present in D. saccharalis and absent in D. flavipennella, contributing to differences in ITS 2 length in the species. An additional 367-bp band was attributed to Cotesia spp. The differences among ITS 2 from D. flavipennella, D. saccharalis, and Cotesia sp were sufficient to identify them on electrophoresis gel and sequencing. The presence of Cotesia sp traits in adult D. flavipennella showed possible host refractoriness, but further studies are necessary.

show abstract

Deep sympatric mtDNA divergence in the autumnal moth (Epirrita autumnata)

Cited by 28 publications

References 95 publications

A Transcontinental Challenge — A Test of DNA Barcode Performance for 1,541 Species of Canadian Noctuoidea (Lepidoptera)

A Transcontinental Challenge — A Test of DNA Barcode Performance for 1,541 Species of Canadian Noctuoidea (Lepidoptera)

Untangling taxonomy: a DNA barcode reference library for Canadian spiders

Research Article ITS2 as a molecular marker for the identification of Diatraea saccharalis and D. flavipennella and possible infection with Cotesia spp.

Contact Info

Product

Resources

About