Defective Viral Particles and Viral Disease Processes

Huang, Alice S.; Baltimore, David

doi:10.1038/226325a0

Cited by 660 publications

(427 citation statements)

References 36 publications

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“…Defective-interfering (DI) virus particles accumulate during serial passage of many animal viruses at a high multiplicity of infection (Huang & Baltimore, 1970, 1977, including the alphaviruses Semliki Forest virus (SFV) and Sindbis virus (Bruton & Kennedy, 1976;Schlesinger et al, 1972). All DI viruses have a portion of their genome deleted and can only replicate in the presence of standard (infectious) virus and this interferes with the multiplication of the standard virus.…”

Section: Introductionmentioning

confidence: 99%

Assay of Defective-interfering Semliki Forest Virus by the Inhibition of Synthesis of Virus-specified RNAs

Barrett

Crouch

Dimmock

1981

Journal of General Virology

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SUMMARYWe describe a simple, rapid and reproducible assay for defective-interfering Semliki Forest virus (DI SFV) which is based on the inhibition of synthesis of virus-specified RNAs in SFV-infected cells. Using the assay, we have been able to show that DI virus is generated by a single passage in baby hamster kidney (BHK) cells in an inoculum which contained no detectable DI virus and we have calculated the u.v. target size of the interfering activity.

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Section: Introductionmentioning

confidence: 99%

Assay of Defective-interfering Semliki Forest Virus by the Inhibition of Synthesis of Virus-specified RNAs

Barrett

Crouch

Dimmock

1981

Journal of General Virology

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“…The DI particles of these strains were shown to fulfil the established criteria for DI virus particles (Huang & Baltimore, 1970). The rabies virus DI particles contain part of the virus genome (Wunner & Clark, 1978, 1980 and all of the virion structural polypeptides (W. H. Wunner & H F. Clark, unpublished data); the Di particles replicate in the presence of standard virus and interfere with the replication of homologous standard virus.…”

Section: Discussionmentioning

confidence: 95%

“…The possibility that defective interfering (DI) particles may play a significant role in determining the outcome of virus infections in vivo has been strongly advocated (Huang & Baltimore, 1970). There is indirect evidence that DI particles may be associated with attenuation of virus infection in some animal systems (Darnell & Koprowski, 1974;Woodward & Smith, 1975).…”

Section: Introductionmentioning

confidence: 99%

Defective Interfering Particles of Fixed Rabies Viruses: Lack of Correlation with Attenuation or Auto-interference in Mice

Clark

Parks

Wunner

1981

Journal of General Virology

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“…Persistent viral infections have been linked to temperature-sensitive mutations (Preble & Youngner, 1975) and to DI particles (Huang & Baltimore, 1970). The serial passage of a wellcharacterized plaque-purified isolate of HZ-1 virus (B1) resulted not only in a decrease in the level of infectious virus (indicating the presence of yon Magnus particles) but also in a virus population capable of establishing persistence.…”

Section: Discussionmentioning

confidence: 99%

Defective Particles from a Persistent Baculovirus Infection in Trichoplusia ni Tissue Culture Cells

Burand

Wood

Summers

1983

Journal of General Virology

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SUMMARYAn HZ-1 baculovirus clone isolated from persistently infected Trichoplusia ni tissue culture cells was homogeneous as determined by particle morphology and restriction enzyme analysis of virus DNA. Re-establishment of a persistent infection of TN-368 cells with this clone (B1) was unsuccessful. There was a 99~o decrease in infectious virus titre following 10 passages in tissue culture at a high multiplicity of infection, and the 10th passage virus population was capable of establishing a persistently infected cell line (TN-B1). Based on particle morphology, restriction enzyme analysis, and focus-forming plaque assays, the virus recovered from TN-B1 cells included a high percentage of defective virus particles which did not interfere with the infection of second-passage BI virus particles. The role of defective HZ-1 virus particles in the establishment of persistent infections is discussed.

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Defective Viral Particles and Viral Disease Processes

Cited by 660 publications

References 36 publications

Assay of Defective-interfering Semliki Forest Virus by the Inhibition of Synthesis of Virus-specified RNAs

Assay of Defective-interfering Semliki Forest Virus by the Inhibition of Synthesis of Virus-specified RNAs

Defective Interfering Particles of Fixed Rabies Viruses: Lack of Correlation with Attenuation or Auto-interference in Mice

Defective Particles from a Persistent Baculovirus Infection in Trichoplusia ni Tissue Culture Cells

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