Defects in myoglobin oxygenation in KATP-deficient mouse hearts under normal and stress conditions characterized by near infrared spectroscopy and imaging

Abstract

Defects in myoglobin oxygenation in K ATP -deficient mouse hearts under normal and stress conditions characterized by near infrared spectroscopy and imaging Abstract BackgroundDisruption of ATP-sensitive potassium (K ATP ) channel activity results in the development of dilated cardiomyopathy in response to different forms of stress, likely due to the underlying metabolic defects. To further understand the role of Kir6.2-containing channels in the development of cardiac disease, we analysed the left ventricular (LV) wall oxygenation and the physiologic responses induced by acute stress in non-dilated Kir6.2 −/− hearts. MethodsControl (C57BL6) and Kir6.2 −/− mouse hearts were perfused in constant flow Langendorff mode with Krebs-Henseleit buffer. Myocardial oxygenation was evaluated using a newly developed technique, near infrared spectroscopic imaging (NIRSI) of the myoglobin (Mb) oxygen saturation parameter (OSP, ratio of oxy-to total Mb). Results2,4-dinitrophenol (DNP, 50-µM) and isoproterenol (0.1-µM) failed to produce a transient vasodilatory response and caused a significant diastolic pressure increase in Kir6.2 −/− hearts. DNP strongly suppressed contractile function in both groups and induced severe mean OSP decreases in Kir6.2 −/− hearts. Isoproterenol-induced decreases in OSP were similar despite the lack of contractile function stimulation in the Kir6.2 −/− group. The index of OSP spatial heterogeneity (relative dispersion, RD) was lower by 15% in the Kir6.2 −/− group at the baseline conditions. Recovery after stress caused reduction of RD values by 20% (DNP) and 8% (isoproterenol) in controls; however, these values did not change in the Kir6.2 −/− group. Conclusions1) NIRSI can be used to analyse 2-D dynamics of LV oxygenation in rodent models of cardiomyopathy; 2) Kir6.2-containing K ATP channels play an important role in maintaining myocardial oxygenation balance under acute stress conditions and in post-stress recovery.

Near‐Infrared In Vivo Spectroscopic Imaging: Biomedical Research and Clinical Applications

“…This work, reported here for the first time [58], shows that near-IR spectroscopic imaging is capable of providing highresolution oxygenation maps of KHB-perfused mouse hearts. As such, the technique can be used to detect perfusion and metabolism deficits and to characterize microvessel damage in rodent models of cardiac diseases.…”

Review of spectral imaging technology in biomedical engineering: achievements and challenges

“…On the other hand, LCTF is a solid-state instrument that utilizes electronically controlled liquid crystal cells to transmit light with a specific wavelength and the elimination of all other wavelengths. The LCTF approach also has been used in coupling with the microscope, 65,[89][90][91][92] fundus microscope, 114,[117][118][119] and camera lens [56][57][58]60,62,64,[68][69][70]77 to create spectral image cubes for biological imaging in vivo and in vitro.…”

Rhodamine 800 as a near-infrared fluorescent deposition flow tracer in rodent hearts

“…Myocardial oxygenation is characterized by oxygen saturation parameter (OSP) defined as a ratio of oxygenated to total myoglobin concentration. 25,26 In the present work, OSP was determined using NIR diffuse reflectance measurements in the 700 to 965 nm range as described elsewhere. 21,27 The data for 23 spectra measured from the surface of beating and KCl-arrested rat hearts were averaged: OSP = 81 ± 11% for normal and OSP = 0% for ischemic areas of the hearts.…”