Defects in the Secretory Pathway and High Ca<sup>2+</sup>Induce Multiple P-bodies

Kilchert, Cornelia; Weidner, Julie; Prescianotto-Baschong, Cristina; Spang, Anne

doi:10.1091/mbc.e10-02-0099

Cited by 59 publications

(100 citation statements)

References 60 publications

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“…We thus find it likely that the effects on RNA granules that we observe on calcium homeostasis perturbations are functionally linked to and funneled through the glucose-sensing system and PKA. Interestingly, while this work was in preparation, a functional link in S. cerevisiae between calcium homeostasis perturbations and RNA granule formation was also reported (Kilchert et al 2010). There is a notable difference between the species in that the addition of Ca 2+ alone does not induce RNA granule formation in S. pombe, whereas in the budding yeast, exposure to increased external Ca 2+ robustly increases RNA granule formation.…”

Section: Relationship Between Pka1 Signaling and Perturbation Of Calcmentioning

confidence: 88%

Cellular stress induces cytoplasmic RNA granules in fission yeast

Nilsson¹,

Sunnerhagen²

2010

RNA

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Severe stress causes plant and animal cells to form large cytoplasmic granules containing RNA and proteins. Here, we demonstrate the existence of stress-induced cytoplasmic RNA granules in Schizosaccharomyces pombe. Homologs to several known protein components of mammalian processing bodies and stress granules are found in fission yeast RNA granules. In contrast to mammalian cells, poly(A)-binding protein (Pabp) colocalizes in stress-induced granules with decapping protein.After glucose deprivation, protein kinase A (PKA) is required for accumulation of Pabp-positive granules and translational downregulation. This is the first demonstration of a role for PKA in RNA granule formation. In mammals, the translation initiation protein eIF2a is a key regulator of formation of granules containing poly(A)-binding protein. In S. pombe, nonphosphorylatable eIF2a does not block but delays granule formation and subsequent clearance after exposure to hyperosmosis. At least two separate pathways in S. pombe appear to regulate stress-induced granules: pka1 mutants are fully proficient to form granules after hyperosmotic shock; conversely, eIF2a does not affect granule formation in glucose starvation. Further, we demonstrate a Pka1-dependent link between calcium perturbation and RNA granules, which has not been described earlier in any organism.

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Section: Relationship Between Pka1 Signaling and Perturbation Of Calcmentioning

confidence: 88%

Cellular stress induces cytoplasmic RNA granules in fission yeast

Nilsson¹,

Sunnerhagen²

2010

RNA

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“…We have shown earlier that P-bodies in yeast are 109 in very close proximity to the endoplasmic reticulum (ER) and that they fractionate with ER 110 membranes (Kilchert et al, 2010;Weidner et al, 2014). To explore the mRNA content of P-bodies, 111 either Dcp2p or Scd6p, which are part of the 5' and the 3'UTR-associated complex of P-bodies, 112 respectively, were chromosomally tagged with a His6-biotinylation sequence-His6 tandem tag 113 (HBH) (Tagwerker et al, 2006;Weidner et al, 2014).…”

Section: A Novel Methods To Isolate Rnas Sequestered Into P-bodies 103mentioning

confidence: 99%

“…P-bodies were either induced through 114 glucose starvation or through the addition of CaCl2 or NaCl. We chose CaCl2 as stressor because 115 secretory pathway mutants induce P-bodies through a Ca 2+ /calmodulin-dependent pathway, 116 which is mimicked by the addition of Ca 2+ to the medium (Kilchert et al, 2010). Notably, this 117 induction pathway is different from the one employed by the cell upon glucose starvation.…”

Section: A Novel Methods To Isolate Rnas Sequestered Into P-bodies 103mentioning

confidence: 99%

“…Rows and columns correspond to stress conditions and pathways, respectively, and the negative logarithms of q-values are color-coded from blue (low) to red (high). 7 time point (Kilchert et al, 2010). Libraries for RNA-Seq were prepared in two ways: either using 127 PAGE purification with radiolabeled mRNAs or using a column-based purification method (Table 128 S1).…”

Section: A Novel Methods To Isolate Rnas Sequestered Into P-bodies 103mentioning

confidence: 99%

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Context-dependent deposition and regulation of mRNAs in P-bodies

Wang

Schmich

Weidner

et al. 2017

Preprint

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24 25Cells respond to stress by remodeling their transcriptome through transcription and degradation. 26Xrn1p-dependent degradation in P-bodies is the most prevalent pathway. Yet, P-bodies may 27 facilitate not only decay but also act as storage compartment. However, which and how mRNAs 28 are selected into different degradation pathways and what determines the fate of any given mRNA 29 in P-bodies remain largely unknown. We devised a new method to identify both common and 30 stress-specific mRNA subsets associated with P-bodies. mRNAs targeted for degradation to P-31 bodies, decayed with different kinetics. Moreover, the localization of a specific set of mRNAs to 32

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“…Depending upon the stress that is encountered, the size and number of P bodies varies. For example, glucose starvation induces a few relatively large foci, whereas high salt and high Ca 2+ cause the formation of smaller but more numerous P bodies Kilchert et al, 2010). The reason for the different responses is not clear.…”

Section: Introductionmentioning

confidence: 99%

The polysome-associated proteins Scp160 and Bfr1 prevent P body formation under normal growth conditions

Weidner

Wang

Prescianotto-Baschong

et al. 2014

Journal of Cell Science

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Numerous mRNAs are degraded in processing bodies (P bodies) in Saccharomyces cerevisiae. In logarithmically growing cells, only 0-1 P bodies per cell are detectable. However, the number and appearance of P bodies change once the cell encounters stress. Here, we show that the polysome-associated mRNA-binding protein Scp160 interacts with P body components, such as the decapping protein Dcp2 and the scaffold protein Pat1, presumably, on polysomes. Loss of either Scp160 or its interaction partner Bfr1 caused the formation of Dcp2-positive structures. These Dcp2-positive foci contained mRNA, because their formation was inhibited by the presence of cycloheximide. In addition, Scp160 was required for proper P body formation because only a subset of bona fide P body components could assemble into the Dcp2-positive foci in Dscp160 cells. In either Dbfr1 or Dscp160 cells, P body formation was uncoupled from translational attenuation as the polysome profile remained unchanged. Collectively, our data suggest that Bfr1 and Scp160 prevent P body formation under normal growth conditions.

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Defects in the Secretory Pathway and High Ca²⁺Induce Multiple P-bodies

Abstract: This manuscript demonstrates for the first time that P-body (PB) formation in response to stress is a regulated process, and that at least two different pathways drive PB assembly. We provide evidence that PB formation and translation attenuation are not strictly linked.

Cited by 59 publications

References 60 publications

Cellular stress induces cytoplasmic RNA granules in fission yeast

Cellular stress induces cytoplasmic RNA granules in fission yeast

Context-dependent deposition and regulation of mRNAs in P-bodies

The polysome-associated proteins Scp160 and Bfr1 prevent P body formation under normal growth conditions

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Defects in the Secretory Pathway and High Ca2+Induce Multiple P-bodies

Abstract: This manuscript demonstrates for the first time that P-body (PB) formation in response to stress is a regulated process, and that at least two different pathways drive PB assembly. We provide evidence that PB formation and translation attenuation are not strictly linked.

Cited by 59 publications

References 60 publications

Cellular stress induces cytoplasmic RNA granules in fission yeast

Cellular stress induces cytoplasmic RNA granules in fission yeast

Context-dependent deposition and regulation of mRNAs in P-bodies

The polysome-associated proteins Scp160 and Bfr1 prevent P body formation under normal growth conditions

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Defects in the Secretory Pathway and High Ca²⁺Induce Multiple P-bodies