Defence responses in leaves of resistant and susceptible pepper (Capsicum annuum L.) cultivars infected with different inoculum concentrations of Phytophthora capsici Leon

Koç, Esra; Üstün, Ayşen Sülün; İşlek, Cemil; Arıcı, Yeliz Kaşko

doi:10.1016/j.scienta.2011.02.008

Cited by 23 publications

(15 citation statements)

References 45 publications

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“…The deposition of phenolic compounds observed during the melon-C. lagenarium interaction correlated with the induction of PAL, an important enzyme in the biosynthesis of related phenolic compounds in cucumber [47], tomato [21], pepper [48,49], cowpea [22] and wheat [50]. PAL activity was higher in the resistant cultivar and correlated with higher levels of antifungal phenolic compounds.…”

Section: Discussionmentioning

confidence: 90%

Defence responses in leaves of resistant and susceptible melon (Cucumis melo L.) cultivars infected with Colletotrichum lagenarium

Guest

2013

Physiological and Molecular Plant Pathology

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Section: Discussionmentioning

confidence: 90%

Defence responses in leaves of resistant and susceptible melon (Cucumis melo L.) cultivars infected with Colletotrichum lagenarium

Guest

2013

Physiological and Molecular Plant Pathology

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“…Zoospores were induced to release by incubating the culture plates in sterile water at 4°C at room temperature for 1 hour. The zoospores were collected and were filtered through Whatman No: 54 to remove sporangial cases and mycelia (Ward, 1976;Koç et al, 2011). Zoospore is a motile asexual spore that uses a flagellum for motion and it is difficult to count, therefore two droplets of lactophenol aniline blue were added to the zoospore suspensions.…”

Section: Methodsmentioning

confidence: 99%

“…Zoospore is a motile asexual spore that uses a flagellum for motion and it is difficult to count, therefore two droplets of lactophenol aniline blue were added to the zoospore suspensions. After the movement of the zoospores which are moving was stopped with the aniline blue with lactophenol of 3% for spore concentration count, the counting was performed with a hemocytometer (Koç et al, 2011). The concentration was then adjusted to 10 4 zoospores per milliliter and one of the most adequate zoospore concentration causing the disease in the pepper was prepared by dilution with sterile distilled water containing 1-2 drops per L TWEEN ® 20 (Sigma-Aldrich).…”

Section: Methodsmentioning

confidence: 99%

Pretreatment with spermidine and proline reverses inhibitory effects of Phytophthora capsici stress in pepper

Koç

Arıcı

İşlek

2016

Zemdirbyste-Agriculture

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Phytophthora blight (Phytophthora capsici Leon.), which is one of the most important disease factors incurring great economic losses, causes root crown rot. When pepper seedlings were pretreated with 0.1 mM spermidine (spd), 1 mM spd, 0.1 mM spd + 1 mM proline, 0.1 mM spd + 10 mM proline, 1 mM spd + 1 mM proline and 1 mM spd + 10 mM proline and then exposed to P. capsici, they improved their tolerance to subsequent P. capsici-induced oxidative damage. It was detected that cultivar 'Sirena RZ F1' was resistant to P. capsici and it was determined that cultivar 'Sera Demre-8' exhibited sensitive reaction. P. capsici stress caused an increase in total phenolic, malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ) levels, as well as peroxidase (POX) and polyphenol oxidase (PPO) activities in leaves of pepper seedlings. Application of exogenous spermidine and spermidine + proline further increased PPO and POX activity, total phenolic amount. On the other hand, these applications in two cultivars and all days decreased the disease severity, necrosis length and MDA and H 2 O 2 amounts. These data show that antioxidants, especially polyphenol oxidase, peroxidase and total phenolic appear to protect pepper seedlings against stress-related damage. Moreover, spermidine and spermidine + proline have the potential to scavenge directly free radicals and promote the activity of antioxidant enzymes in pepper seedlings under P. capsici stress. In addition, it was also identified that especially 0.1 mM spd, 1 mM spd and 0.1 mM spd + 1 mM proline applications decreased the colony growth of P. capsici and the amount of zoospores. Therefore, we suggest that spermidine and spermidine + proline could play a protective role in protecting the pepper seedlings from P. capsici stress.

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“…Six bunches were put in each glass bottle with a wide mouth so that each bottle contained 30 seedlings. Bunches inside the bottle with an opening just wide enough for plant bunches were supported with the cotton wrapped around them and incubated for 3 days in a plant breeding chamber adjusted to 22 ± 3°C, 60% humidity, and 14 h of a light period to adapt to changing environmental conditions (Koç et al 2011).…”

Section: Methodsmentioning

confidence: 99%

“…Distilled water treatment was performed in the control groups. Inoculation procedure (Koç et al 2011) was done 72 h after treatment. Under the same conditions, random samples were taken on the first, third and fifth days according to the random block design model.…”

Section: Methodsmentioning

confidence: 99%

Exogenous application of spermidine enhanced tolerance of pepper against Phytophthora capsici stress

Koç¹

2015

Plant Prot. Sci.

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Koç E. (2015): Exogenous application of spermidine enhanced tolerance of pepper against Phytophthora capsici stress. Plant Protect. Sci., 51: 127-135.The effect of exogenous spermidine -Spd (0.1 and 1 mM) on the relation between polyaminoxidase (PAO), diaminoxidase (DAO), H 2 O 2 , and malondialdehyde (MDA) in three cultivars of pepper (Capsicum annum L.) exhibiting different tolerance to P. capsici stress: KM-Hot (P. capsici-tolerant), , and CM-334 (P. capsici-highly resistant) was investigated. The 0.1 mM Spd pre-treatment led to an increase in DAO activity on the third day in three pepper cultivars under the stress of P. capsici, 1 mM Spd + P. capsici led to an increase in DAO and PAO activities on the fifth day if compared to P. capsici treatment alone. P. capsici alone caused an increase in the amounts of H 2 O 2 at all times in all cultivars and in the amounts of MDA on the third and fifth days in all cultivars. Conversely; under the stress of P. capsici, pre-application of 0.1 mM Spd at all times in KM-Hot and CM-334 cultivars decreased the amount of MDA and H 2 O 2 and on the first and third days in PM-217 cultivar decreased the amount of MDA and H 2 O 2 . This data indicates that exogenous Spd application before inoculation decreases the plasma membrane injury by decreasing the level of H 2 O 2 and regulating the activities of amine oxidase in both P. capsici-sensitive and P. capsici-resistant cultivars of peppers, so it may increase the tolerance of pepper cultivars against P. capsici.

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Defence responses in leaves of resistant and susceptible pepper (Capsicum annuum L.) cultivars infected with different inoculum concentrations of Phytophthora capsici Leon

Cited by 23 publications

References 45 publications

Defence responses in leaves of resistant and susceptible melon (Cucumis melo L.) cultivars infected with Colletotrichum lagenarium

Defence responses in leaves of resistant and susceptible melon (Cucumis melo L.) cultivars infected with Colletotrichum lagenarium

Pretreatment with spermidine and proline reverses inhibitory effects of Phytophthora capsici stress in pepper

Exogenous application of spermidine enhanced tolerance of pepper against Phytophthora capsici stress

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