2020
DOI: 10.1016/j.celrep.2020.02.091
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Defining Epidermal Basal Cell States during Skin Homeostasis and Wound Healing Using Single-Cell Transcriptomics

Abstract: Highlights d scRNA-seq identifies four epidermal basal cell states in homeostatic adult skin d Computational analysis supports a ''hierarchical'' model of epidermal homeostasis d Basal cell states are metabolically distinct and spatially partitioned in wounded skin d Epidermal basal cells show enhanced cell fate and state plasticity during wound healing

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Cited by 179 publications
(142 citation statements)
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“…The most commonly used include Rgs5 (Bondjers et al, 2003), Pdgfrβ (Armulik et al, 2011), Nestin (Birbrair et al, 2013a), Tbx18 , CD146 (Dvoretskiy et al, 2019), and Neural/glial antigen 2 (Ng2) (Armulik et al, 2011). However, none of these markers are pericyte specific as each marker can identify multiple dermal cell populations; for example Pdgfrβ is also expressed by adventitial fibroblasts (Stapor et al, 2014) and CD146 is also expressed by endothelial cells (Crisan et al, 2008;Guerrero-Juarez et al, 2019;Gay et al, 2020;Haensel et al, 2020). Amongst the markers Ng2 is the most widely documented because it consistently labels cells with pericyte features on multiple types of vasculature in different organs (Ozerdem et al, 2001;Ozerdem and Stallcup, 2004).…”
Section: Introductionmentioning
confidence: 99%
“…The most commonly used include Rgs5 (Bondjers et al, 2003), Pdgfrβ (Armulik et al, 2011), Nestin (Birbrair et al, 2013a), Tbx18 , CD146 (Dvoretskiy et al, 2019), and Neural/glial antigen 2 (Ng2) (Armulik et al, 2011). However, none of these markers are pericyte specific as each marker can identify multiple dermal cell populations; for example Pdgfrβ is also expressed by adventitial fibroblasts (Stapor et al, 2014) and CD146 is also expressed by endothelial cells (Crisan et al, 2008;Guerrero-Juarez et al, 2019;Gay et al, 2020;Haensel et al, 2020). Amongst the markers Ng2 is the most widely documented because it consistently labels cells with pericyte features on multiple types of vasculature in different organs (Ozerdem et al, 2001;Ozerdem and Stallcup, 2004).…”
Section: Introductionmentioning
confidence: 99%
“…(Fig.3E to G). Indeed, pathways involved in active wound healing and tissue repair were found to be enriched in both branches; these included EGF (Areg, Nrg1, Egr1, Vav3, Tfap2a) [42][43][44][45] , AP-1 (Junb, Fos) 11,46,47 , and FGF (Fgfr3, Fgfbp1) 48 .…”
Section: Single Cell Rna Sequencing Analysis Captures the Regenerativmentioning
confidence: 99%
“…Basal cells are essential for maintaining the integrity of skin and promoting wound healing. Haensel et al [ 12 ] used scRNA-seq combined with RNA in situ detection (RNAScope) and fluorescence lifetime imaging microscopy (FLIM) to dynamically monitor cell states during skin homeostasis and wound healing. In their study, 3 non-proliferative states and 1 proliferative state with different metabolic profiles were identified in the basal layer during homeostasis, and were spatially separated in wound epithelialization.…”
Section: Wound Healingmentioning
confidence: 99%