Defining Reference Ranges for Serum Anti-Müllerian Hormone on a Large Cohort of Normozoospermic Adult Men Highlights New Potential Physiological Functions of AMH on FSH Secretion and Sperm Motility

Benderradji, Hamza; Barbotin, Anne‐Laure; Leroy-Billiard, M.; Prasivoravong, Julie; Marcelli, F.; Decanter, Christine; Robin, Geoffroy; Mitchell, Valérie; Rigot, Jean-Marc; Bongiovanni, Antonino; Sauvé, Florent; Buée, Luc; Maurage, Claude‐Alain; Cartigny, Maryse; Villers, Arnauld; Prévot, Vincent; Catteau-Jonard, Sophie; Sergeant, Nicolas; Giacobini, Paolo; Pigny, Pascal; Leroy, Clara

doi:10.1210/clinem/dgac218

Cited by 10 publications

(14 citation statements)

References 43 publications

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“…Ideally, the observed differences in reproductive hormones between AMH groups could be supported by evaluation of testis histology, but despite this limitation, it is unlikely that men with low serum AMH have more immature Sertoli cells as they in theory would produce higher levels of AMH than mature Sertoli cells [ 3 , 11 ]. Moreover, our findings are in accordance with a recent study showing that serum AMH is positively associated with sperm production, serum FSH, and serum inhibin B in a cohort of normozoospermic men [ 12 ]. Several other studies have measured serum AMH in both fertile and infertile men but most of these cohorts are relatively small.…”

Section: Discussionsupporting

confidence: 93%

“…However, the association between serum AMH and sperm production was not inferior to the link found with serum inhibin B and sperm production. Furthermore, serum AMH was strongly associated with serum inhibin B and serum FSH, which are in line with previous studies [ 4 , 12 , 14 ] and support that serum AMH is a marker of Sertoli cell function. We found that combined low levels of serum AMH and high levels of serum FSH tend to be a better marker for semen quality compared to AMH alone, though only found in up to 16% of infertile men referred for andrological workup prior to ICSI.…”

Section: Discussionsupporting

confidence: 92%

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Low serum anti-Müllerian hormone is associated with semen quality in infertile men and not influenced by vitamin D supplementation

Holt

Yahyavi

Kooij

et al. 2023

BMC Med

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Background Anti-Müllerian hormone (AMH) is released by testicular Sertoli cells and of great importance during fetal male sexual development, but less is known about the role of circulating AMH during adulthood. In vitro studies have shown that vitamin D may induce AMH transcription, but a controlled trial investigating the possible effect of vitamin D on serum AMH has not been conducted in men. Methods A single-center, double-blinded, randomized placebo-controlled clinical trial (NCT01304927) conducted in Copenhagen, Denmark. A total of 307 infertile men were included and randomly assigned (1:1) to a single dose of 300,000 IU cholecalciferol followed by 1400 IU cholecalciferol + 500 mg of calcium daily (n = 151) or placebo (n = 156) for 150 days. Difference in serum AMH was a predefined secondary endpoint. Explorative outcomes were associations between serum AMH and gonadal function in infertile men. The primary endpoint was difference in semen quality and has previously been published. Results Infertile men in the lowest AMH tertile had significantly lower sperm concentration (∆T3-1 16 mill/mL (228%); P < 0.001), sperm count (∆T3-1 55 million (262%); P < 0.001), motile sperm count (∆T3-1 28 million (255%); P < 0.001), progressive motile sperm count (∆T3-1 18 million (300%); P < 0.001), testis size (∆T3-1 2.7 mL (16%); P < 0.001), serum inhibin B (∆T3-1 72 pg/mL (59%); P < 0.001), inhibin B/FSH ratio (∆T3-1 48 (145%); P < 0.001), and higher FSH (∆T3-1 2.6 (38%); P < 0.001) than the tertile of infertile men with highest serum AMH. Vitamin D supplementation had no effect on serum AMH compared with placebo treatment. Conclusions In infertile men, low serum AMH is associated with severely impaired gonadal function illustrated by poor semen quality and lower testosterone/LH ratio. Serum AMH in infertile men was not influenced by vitamin D supplementation.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

Low serum anti-Müllerian hormone is associated with semen quality in infertile men and not influenced by vitamin D supplementation

Holt

Yahyavi

Kooij

et al. 2023

BMC Med

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“…Antimüllerian hormone (AMH) is an indicator for detecting ovarian function, but it is also one of the most important indicators for evaluating male fertility. It has been shown that AMH levels are positively correlated with total sperm count, concentration, and forward motility [29]. In this study, the serum levels of these hormones were determined at two different months of age in Qianbei goats, and the levels at age 9 months were all extremely signi cantly higher than those at age 3 months (P < 0.01).…”

Section: Discussionmentioning

confidence: 66%

Comparison of the gene expression profile of testicular tissue before and after sexual maturity in Qianbei Ma goats

Chen,

Guo

et al. 2023

Preprint

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Background:In this work that we took 3-month-old (presexual maturity) and 9-month-old (post-sexual maturity) goats as test subjects and observed the changes in the morphology of testicular tissues through HE staining; detected the serum testosterone,luteinizing hormone,follicle stimulating hormone and anti-Müllerianhormone content; analysedthe mRNA expression profiles of Qianbei Ma goat testes before and after sexual maturity by transcriptomics; and screened for differences in the expression of genes before and after sexual maturity. Results:The results showed that the area and diameter of spermatogenic tubules were larger at age 9 months than at age 3 months, and the number of spermatocytes, interstitial cells, spermatogonia and secondary spermatocytes in the lumen of the tubules was increased compared with that at age 3 months. The appearance of spermatozoa at age 3 months indicated that puberty had begun in Qianbei goats. The Elasa test for testosterone, luteinizing hormone, follicle stimulating hormone and anti-Müllerian hormone showed that the levels of these hormones in the serum at age 9 months were all extremely significantly higher than those at age 3 months (p<0.01). we performed RNA-seq analysis 490 were found to be differentially expressed genes（DEGs）at the criteria of |log2(foldchange)| > 1 and pvalue＜0.05. of which 233 genes were upregulated and 257 genes were downregulated. Through GO and KEGG enrichment analysis, the candidate genes, PRSS58, ECM1, WFDC8 and LHCGR. Conclusions:This work that may be involved in the regulation of testicular development and spermatogenesis in Qianbei Ma goats were identified, providing a theoretical basis and data support for later studies on the influence of testicular development and spermatogenesis before and after sexual maturity in Qianbei Ma goats.

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“…Luteinizing hormone (LH) promotes testicular development and eventual maturation of spermatogenesis [28], and follicle-stimulating hormone (FSH) has a major role in males by acting synergistically with testosterone to maximize sperm production [29]. Studies have shown that AMH levels are positively correlated with total sperm count, concentration and positive motility [30]. In this study, the serum levels of these hormones were determined at two different months of age in Qianbei Ma goats, and the levels at age 9 months were all extremely significantly higher than those at age 3 months (P < 0.01), which was similar to the study of Zhang [7].…”

Section: Discussionmentioning

confidence: 99%

Comparison of the gene expression profile of testicular tissue before and after sexual maturity in Qianbei Ma goats

Chen,

Guo

et al. 2024

BMC Vet Res

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Background With long-term research on the reproductive ability of Qianbei Ma goat, we found that the puberty of the male goats comes at the age of 3 months and reaches sexual maturity at 4 months,the male goats are identified as physically mature at 9 months and able to mate. Compared with other kinds of breeds of goats, Qianbei Ma goat is featured with more faster growth and earlier sexual maturity.Therefore, in order to explore the laws of growth of Qianbei Ma goat before sexual maturity(3-month-old)and after sexual maturity (9-month-old). The testicular tissue was collected to explore their changes in morphology through HE staining, the serum was collected to detect the hormone content, and the mRNA expression profile of the testis was analyzed by transcriptomics. In this way, the effect of testicular development on the reproduction of Qianbei ma goats was further analyzed. Results The results showed that the area and diameter of spermatogenic tubules were larger at 9 months than 3 months, and the number of spermatocytes, interstitial cells, spermatogonia and secondary spermatocytes in the lumen of the tubules showed a similar trend. The appearance of spermatozoa at age 3 months indicated that puberty had begun in Qianbei Ma goats. The Elasa test for testosterone, luteinizing hormone, follicle stimulating hormone and anti-Müllerian hormone showed that the levels of these hormones in the serum at age 9 months were all highly significantly different than those at age 3 months (P < 0.01). There were 490 differentially expressed genes (DEGs) between the (|log2(fold change)| > 1 and p value < 0.05) 3-month-old and 9-month-old groups, of which 233 genes were upregulated and 257 genes were downregulated (3 months of age was used as the control group and 9 months of age was used as the experimental group). According to the GO and KEGG enrichment analyses of DEGs, PRSS58, ECM1, WFDC8 and LHCGR are involved in testicular development and androgen secretion, which contribute to the sexual maturation of Qianbei Ma goats. Conclusions Potential biomarker genes and relevant pathways involved in the regulation of testicular development and spermatogenesis in Qianbei Ma goats were identified, providing a theoretical basis and data support for later studies on the influence of testicular development and spermatogenesis before and after sexual maturity in Qianbei Ma goats.

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Defining Reference Ranges for Serum Anti-Müllerian Hormone on a Large Cohort of Normozoospermic Adult Men Highlights New Potential Physiological Functions of AMH on FSH Secretion and Sperm Motility

Cited by 10 publications

References 43 publications

Low serum anti-Müllerian hormone is associated with semen quality in infertile men and not influenced by vitamin D supplementation

Low serum anti-Müllerian hormone is associated with semen quality in infertile men and not influenced by vitamin D supplementation

Comparison of the gene expression profile of testicular tissue before and after sexual maturity in Qianbei Ma goats

Comparison of the gene expression profile of testicular tissue before and after sexual maturity in Qianbei Ma goats

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