Defining the anatomy of the Rangifer tarandus sex chromosomes

Lee, C.; Griffin, Darren K.; O’Brien, Patrícia C. M.; Yang, Fengtang; Lin, Chyi-Chyang; Ferguson‐Smith, Malcolm A.

doi:10.1007/s004120050281

Cited by 14 publications

(18 citation statements)

References 24 publications

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“…This result suggests that some sequences on the X euchromatin share homology with the Y euchromatin. This ¢nding is not surprising as mammalian sex chromosomes share the pseudoautosomal region, involved in pairing and segregation of chromosomes in meiosis, which showed crosshybridization with painting probes of the X (Lee et al 1998) or the Y chromosomes (Howell et al 1994). However, sex chromosomes of M. cabrerae, as in other species of the same genus, are asynaptic (Jime¤ nez et al 1991; for a review see Megı´as-Nogales et al 2003).…”

Section: Discussionmentioning

confidence: 82%

X chromosome painting in Microtus: Origin and evolution of the giant sex chromosomes

et al. 2004

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Sex chromosomes in species of the genus Microtus present some characteristic features that make them a very interesting group to study sex chromosome composition and evolution. M. cabrerae and M. agrestis have enlarged sex chromosomes (known as 'giant sex chromosomes') due to the presence of large heterochromatic blocks. By chromosome microdissection, we have generated probes from the X chromosome of both species and hybridized on chromosomes from six Microtus and one Arvicola species. Our results demonstrated that euchromatic regions of X chromosomes in Microtus are highly conserved, as occurs in other mammalian groups. The sex chromosomes heterochromatic blocks are probably originated by fast amplification of different sequences, each with an independent origin and evolution in each species. For this reason, the sex heterochromatin in Microtus species is highly heterogeneous within species (with different composition for the Y and X heterochromatic regions in M. cabrerae) and between species (as the composition of M. agrestis and M. cabrerae sex heterochromatin is different). In addition, the X chromosome painting results on autosomes of several species suggest that, during karyotypic evolution of the genus Microtus, some rearrangements have probably occurred between sex chromosomes and autosomes.

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Section: Discussionmentioning

confidence: 82%

X chromosome painting in Microtus: Origin and evolution of the giant sex chromosomes

et al. 2004

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“…If the ancestral X-and Y-chromosomes in an ancestral species were once homomorphic, one could speculate that the current Y-chromosome is derived mainly from an interstitial site on the ancestral homomorphic sex chromosome-where satellite V sequences can now be observed. Such homology of a heterochromatin segment between the sex chromosomes also had been reported in the cervid species Rangifer tarandus (Lee et al 1998).…”

Section: Discussionmentioning

confidence: 83%

Karyotypic evolution of a novel cervid satellite DNA family isolated by microdissection from the Indian muntjac Y-chromosome

Cheng

Hsieh

et al. 2005

Chromosoma

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A minilibrary was constructed from DOP-PCR products using microdissected Y-chromosomes of Indian muntjac as DNA templates. Two microclones designated as IM-Y4-52 and IM-Y5-7 were obtained from negative screening of all three cervid satellite DNAs (satellites I, II, and IV). These two microclones were 295 and 382 bp in size, respectively, and shared approximately 70% sequence homology. Southern blot analysis showed that the IM-Y4-52 clone was repetitive in nature with an approximately 0.32-kb register in HaeIII digest. Sequence comparison revealed no similarities to DNA sequences deposited in the GenBank database, suggesting that the microclone sequences were from a novel satellite DNA family designated as cervid satellite V. A subclone of an Indian muntjac BAC clone which screened positive for IM-Y4-52 had a 3,325-bp insert containing six intact monomers, four deleted monomers, and two partial monomers. The consensus sequence of the monomer was 328 bp in length and shared more than 80% sequence homology with every intact monomer. A zoo blot study using IM-Y4-52 as a probe showed that the strong hybridization with EcoRI digested male genomic DNA of Indian muntjac, Formosan muntjac, Chinese muntjac, sambar deer, and Chinese water deer. Female genomic DNA of Indian muntjac, Chinese water deer, and Formosan muntjac also showed positive hybridization patterns. Satellite V was found to specifically localize to the Y heterochromatin region of the muntjacs, sambar deer, and Chinese water deer and to chromosome 3 of Indian muntjac and the X-chromosome of Chinese water deer.

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“…canids [Nash et al, 2001;PienkowskaSchelling et al, 2005], in the reindeer Rangifer tarandus [Lee et al, 1998], and in the tammar wallaby Macropus eugenii [Toder et al, 1997]. These shared sequences could represent either the expected functional PAR where crossing-over occurs or not, as demonstrated in Macropus eugenii , where no pairing is observed within the sex chromosomes during meiosis [Sharp, 1982].…”

Section: Sex Chromosome Pairing and Pseudoautosomal Regions (Pars)mentioning

confidence: 99%

Comparative Analysis by Chromosome Painting of the Sex Chromosomes in Arvicolid Rodents

Acosta

Romero-Fernández²,

Sánchez³

et al. 2010

Cytogenet Genome Res

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Sex chromosome evolution in mammals has been extensively investigated through chromosome-painting analyses. In some rodent species from the subfamily Arvicolinae the sex chromosomes contain remarkable features such as giant size, a consequence of heterochromatic enlargement, or asynaptic behaviour during male meiosis. Here, we have made a comparative study of the sex chromosomes in 6 arvicolid species using different probes from the X and Y chromosomes of 3 species, in order to gain knowledge about intra- or interspecific preservation of euchromatic regions. Our results clearly reveal poor conservation of the euchromatic region of the Y chromosome within these species, while the euchromatin on the X chromosome is extremely well preserved. Furthermore, we detected no clear correlation between the synaptic/asynaptic behaviour of the sex chromosomes, and the presence or absence of sequence homology within their euchromatic regions. Notably, our study has shown a new relationship between the giant sex chromosomes of 2 species, Microtus agrestis and Microtus cabrerae, that is, both X and Y share a novel region of common sequences in the euchromatin that is not present in the other species analysed. This interspecific euchromatic conservation, limited to the giant sex chromosomes, could point towards a common evolutionary origin for the heterochromatic enlargement process that has characterized the evolution of the sex chromosomes in some arvicolid species.

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Defining the anatomy of the Rangifer tarandus sex chromosomes

Cited by 14 publications

References 24 publications

X chromosome painting in Microtus: Origin and evolution of the giant sex chromosomes

X chromosome painting in Microtus: Origin and evolution of the giant sex chromosomes

Karyotypic evolution of a novel cervid satellite DNA family isolated by microdissection from the Indian muntjac Y-chromosome

Comparative Analysis by Chromosome Painting of the Sex Chromosomes in Arvicolid Rodents

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