Definition of rabbit class I and class II MHC gene haplotypes using molecular typing procedures

Marche, Patrice N.; Mc, Rebiere; Laverrière, A; Dw, English; LeGuern, Christian; Tj, Kindt

doi:10.1007/bf00717913

Cited by 17 publications

(5 citation statements)

References 9 publications

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“…Homozygous rabbits showed single-band patterns, and heterozygotes showed two-band patterns (Table 1). Four DQa PvuII bands were found in these rabbits and are referred to as DQaB (6.3 kb), DQaC (5.9 kb), DQaD (4.8 kb), and DQaE (3.0 kb) ( Table 1) as defined previously (10,17). However, we observed that some rabbits showed a three-band pattern ( Table 1), suggesting that new DQa haplotypes are represented in this population of NZW rabbits.…”

supporting

confidence: 52%

“…More-recent studies discovered that both regression and malignant progression of viral warts are linked to rabbit major histocompatibility complex (MHC) class II genes (10) and also to a given viral variant (5) (R. Han, unpublished observations). In order to investigate whether the increased incidence of malignant progression was linked to the MHC class II gene alleles in these rabbits, we typed rabbit MHC class II DQa and DRa genotypes by restriction fragment length polymorphism (RFLP) analysis (17). Three DRa EcoRI bands (Table 1) were found, are referred to as DRaA (6.0 kb), DRaB (5.6 kb), DRaD (4.8 kb), and are in agreement with sizes previously reported (10).…”

mentioning

confidence: 99%

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DNA Vaccination Prevents and/or Delays Carcinoma Development of Papillomavirus-Induced Skin Papillomas on Rabbits

Han

Cladel²,

Reed³

et al. 2000

J Virol

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Malignant progression is a life-threatening consequence of human papillomavirus-associated lesions. In this study, we tested the efficacy of papillomavirus early-gene-based vaccines for prevention of carcinoma development of papillomavirus-induced skin papillomas on rabbits. Rabbit skin papillomas were initiated by infection with cottontail rabbit papillomavirus (CRPV). The papillomas were allowed to grow for 3 months without any treatment intervention. Rabbits were then immunized by gene gun-mediated intracutaneous administration of four DNA plasmids encoding CRPV E1, E2, E6, and E7 genes, respectively. All eight control rabbits receiving vector alone developed invasive carcinoma within 8 to 13 months. In contrast, only two of eight vaccinated rabbits developed carcinoma at 12 and 15 months, respectively. Papilloma growth was suppressed in the majority of vaccinated rabbits but not completely eradicated. These results indicate that gene gun-mediated immunization with papillomavirus early genes may be a promising strategy for prevention of malignant progression of human papillomavirus-associated lesions in humans.High-risk human papillomaviruses (HPVs), such as HPV type 16 (HPV16) and HPV18, first induce benign mucosal and/or cutaneous hyperproliferative lesions that may spontaneously regress or persist and in some patients progress to invasive cancer (32). Epidemiological observations (3) together with those of a functional in vitro study of papillomavirus-encoded oncogenes (20) provide strong evidence that high-risk HPV infection plays a crucial role in the development of anogenital cancer, particularly cervical carcinoma, the second most common cancer in women worldwide. HPV infection is also linked to carcinoma development at other anatomical sites (29). Animal models of papillomavirus infection which mimic features of HPV-induced carcinoma are essential for studying immune responses to these virally induced cancers. One well-characterized model is the cottontail rabbit papillomavirus (CRPV) model (this virus infects cottontail rabbits, its natural host, and domestic rabbits). CRPV infection of New Zealand White (NZW) rabbits initially induces benign skin tumors (papillomas or warts) which may spontaneously regress or progress to invasive carcinoma (16). The natural history of CRPV-induced warts resembles that of human genital HPV infection (16). Recently, the CRPV-rabbit model has been used extensively for developing and testing papillomavirus early-gene-based (13, 14, 26, 31) and late-gene-based (4) vaccines. All these early studies tested the efficacy of vaccination for protection of animals against viral challenge. In our laboratory, rabbit skin warts induced by one CRPV isolate (CRPV-Hershey) showed a very low incidence of spontaneous regression (Ͻ2%) and developed invasive carcinoma on all rabbits with persistent virus infection within about 1 year (7). The model thus provides opportunities to test therapeutic vaccination against both benign (papilloma) and malignant (carcinoma) tumor cells. Our previo...

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supporting

confidence: 52%

mentioning

confidence: 99%

DNA Vaccination Prevents and/or Delays Carcinoma Development of Papillomavirus-Induced Skin Papillomas on Rabbits

Han

Cladel²,

Reed³

et al. 2000

J Virol

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“…Our results indicate that mixed lymphocyte typing in the rabbit does not assure genetic identity for all class I1 genes even though the haplotypes may have had a common genetic origin. The results presented here demonstrate that different RLA-D haplotypes can be identified by RFLP analysis ;Marche et al (1989) have similarly shown that rabbits of different RLA-A haplotypes can be distinguished by RFLP analysis. These analyses, when fully developed, should circumvent the need for serologic reagents and provide a ready means of 'matching ' rabbits at the MHC, thus assuring genetic identity for each locus.…”

Section: Discussionsupporting

confidence: 70%

RABBIT MAJOR HISTOCOMPATIBILITY COMPLEX.: III. MULTIPLE CLASS II DRβ GENES AND RESTRICTION FRAGMENT LENGTH POLYMORPHISM OF THE CLASS II Α AND Β GENES

Sittisombut

Tissot

Knight

1989

Int J Immunogenetics

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SUMMARY Several class II α and β chain genes of the rabbit MHC have been cloned and classified into three distinct subregions, R‐DP, R‐DQ and R‐DR, based on their homology to the corresponding HLA‐DP, ‐DQ and ‐DR genes. The organization of the rabbit MHC class II genes has now been studied in greater detail by analysing genomic DNA of an inbred III/J strain and several other RLA‐D homozygous rabbits, with DNA probes derived from cloned R‐DRβ genes. Eight previously cloned R‐DRβ genes were shown to be allelic forms of five R‐DRβ loci. Genomic blot analyses of DNA from seven rabbits homozygous for different RLA haplotypes revealed that the germline contains a total of approximately seven class II β genes, one DQβ, one DPβ and five DRβ. Extensive allelic polymorphism was identified by RFLP analysis using DQ and DR probes; limited RFLP was observed with DP probes. RFLP analyses allowed us to distinguish two haplotypes which had not been previously distinguished by MLR. Such RFLP analyses will be useful for identifying MHC ‘compatible’ rabbits for various immunobiological studies, including transplantation.

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“…DNA samples from selectively bred rabbits of RLA haplotypes defined by RFLP (12) were amplified using PCR primers based on the published sequence (14). The primers shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Rabbit MHC alleles were originally defined by mixed lymphocyte reactions and cellular transplantation experiments (11). The initial molecular typing of RLA utilized restriction fragment length polymorphisms (RFLP) (12). These studies indicated an unusually high degree of polymorphism in the class II DQA gene suggesting that this may serve as a marker for class II haplotypes.…”

mentioning

confidence: 99%

Improved typing procedure for the polymorphic single‐copy RLA‐DQA gene of the rabbit reveals a new allele

2001

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The DQA gene of the rabbit major histocompatibility complex (MHC, RLA) is highly polymorphic and, in contrast to those reported for other mammalian species, is present as a single copy. These properties allow use of this gene in a method to type the class II locus of RLA by a combination of single-stranded conformational polymorphism (SSCP) and heteroduplex (HD) analysis. Familial segregation of RLA-DQA was shown and RLA class II types for rabbits of unknown pedigree were determined using migration patterns of amplified genomic DNA. Typing results were confirmed in experiments where unknown samples were mixed with products from rabbits of RLA types defined by sequence analysis. These analyses detected an RLA-DQA allele in addition to the five previously described; this new allele is designated RLA-DQA-F.

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Definition of rabbit class I and class II MHC gene haplotypes using molecular typing procedures

Cited by 17 publications

References 9 publications

DNA Vaccination Prevents and/or Delays Carcinoma Development of Papillomavirus-Induced Skin Papillomas on Rabbits

DNA Vaccination Prevents and/or Delays Carcinoma Development of Papillomavirus-Induced Skin Papillomas on Rabbits

RABBIT MAJOR HISTOCOMPATIBILITY COMPLEX.: III. MULTIPLE CLASS II DRβ GENES AND RESTRICTION FRAGMENT LENGTH POLYMORPHISM OF THE CLASS II Α AND Β GENES

Improved typing procedure for the polymorphic single‐copy RLA‐DQA gene of the rabbit reveals a new allele

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