This study aimed to identify challenges in the management of conservation units through a literature review and validate them from the perspective of professionals in the field, considering the context of the Amazon Region. To achieve this, a literature review and a survey were conducted with professionals working in the field of conservation unit management. The collected data were analyzed using the Lawshe method. The results indicated that ten out of fifteen analyzed challenges were validated by the professionals. The validated challenges included: lack of financial resources, urbanization advancement, land conflicts, monitoring and enforcement, climate change, deforestation and illegal exploitation, lack of environmental education, political bureaucracy, conflicts with local communities, and corruption. The validated challenges mainly relate to management issues and the lack of more efficient governance strategies. The findings provide valuable insights for the promotion of sustainable conservation policies in the Amazon.