Deformability and other rheological interactions of red blood cells in electronic cell sizing

Akeson, Stephen P.; Mel, Howard C.

doi:10.3233/bir-1986-23101

Cited by 5 publications

(3 citation statements)

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“…To fix the cell trajectory the hydrodynamic focusing has to be intercalated into electrical transducer. It was shown when normal erythrocytes were measured in an electronic sizer without hydrodynamic focusing, they routinely produced a bimodal apparent‐size distribution [17].…”

Section: Introductionmentioning

confidence: 99%

Erythrocyte lysis and angle‐resolved light scattering measured by scanning flow cytometry result to 48 indices quantifying a gas exchange function of the human organism

et al. 2022

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Molecular/cell level of gas exchange function assumes the accurate measurement of erythrocyte characteristics and rate constants concerning to molecules involved into the CO 2 /O 2 transport. Unfortunately, common hematology analyzers provide the measurement of eight indices of erythrocytes only and say little about erythrocyte morphology and nothing about rate constants of cellular function. The aim of this study is to demonstrate the ability of the Scanning Flow Cytometer (SFC) in the complete morphological analysis of mature erythrocytes and characterization of erythrocyte function via measurement of lysing kinetics. With this study we are introducing 48 erythrocyte indices. To provide the usability of application of the SFC in clinical diagnosis, we formed four categories of indices which are as follows: content/ concentration (9 indices), morphology (26 indices), age (5 indices), and function (8 indices). The erythrocytes of 39 healthy volunteers were analyzed with the SFC to fix the first-ever reference intervals for the new indices introduced. The essential measurable reliability of the presented method is expressed in terms of errors of characteristics of single erythrocytes retrieved from the solution of the inverse lightscattering problem and errors of parameters retrieved from the fitting of the experimental kinetics by molecular-kinetics model of erythrocyte lysis.

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Section: Introductionmentioning

confidence: 99%

Erythrocyte lysis and angle‐resolved light scattering measured by scanning flow cytometry result to 48 indices quantifying a gas exchange function of the human organism

et al. 2022

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“…The specific of RPS technology, used for measuring volume distribution of RBC and ghosts, has been reported elsewhere [30,31]. Coulter-type sizing in RPS produces resistive pulses as particles pass through a current-limiting orifice with a constant current maintained across it.…”

Section: Resistive Pulse Spectroscopy (Rps)mentioning

confidence: 99%

“…In the present study, a cylindrical, 50 µm long and 50 µm in diameter, orifice was used in a system of transducers provided for the almost complete hydrodynamic focusing of cells. To exclude significant deformation of cell membranes, the flow rate in the experiments was less than 1 m\s [31]. Each measurement cycle analysed 1i2"& cells and the current across the orifice did not exceed 0.2 mA.…”

Section: Resistive Pulse Spectroscopy (Rps)mentioning

confidence: 99%

Protection by chlorpromazine, albumin and bivalent cations against haemolysis induced by melittin, [Ala-14]melittin and whole bee venom

Rudenko

Nipot

1996

Biochemical Journal

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The ability of the peptides melittin, [Ala-14]melittin (P14A) and whole bee venom to lyse red blood cells (RBC) and to cause shape transformation, binding, partitioning and changes in volume of the cells during haemolysis, as well as the action of the bivalent cations Zn2+ and Ca2+, chlorpromazine, albumin and plasma on the peptide-induced haemolysis of RBC in high ionic-strength solution, have been investigated. The protective effect of all inhibitors depends on whether they have been added to the media before or after the cells. When added before the cells they reduced significantly the rate of peptide-induced haemolysis and shape transformation. The effect was maximal when agents acted simultaneously after introduction of the cells into the media containing both inhibitors and peptides. Incubation of the cells in isotonic solution before the addition of peptides enhanced 2-3-fold the RBC susceptibility (i.e. rate of haemolysis) to lytic action of the same amount of peptides, and increased the order of the haemolytic reaction, although the power law coefficient did not exceed a value of 2 for all peptides, suggesting that haemolysis is attributable to the monomeric or dimeric forms of the peptides. Partition coefficients were of the order of approximately 10(6) M-1, and P14A possessed a value 3-fold larger compared with melittin and bee venom, which correlated with its enhanced haemolytic activity. The protective action of inhibitors against peptide-induced haemolysis has been explained on the basis of their ability to compete with peptide binding at an early stage of peptide-membrane interaction, and not as a result of inhibition of a pre-existing peptide-induced pore. Whereas melittin increased the volume of RBC during haemolysis, P14A, melittin in the presence of phospholipase A2 or bee venom, reduced the volume in a concentration-dependent manner. The present data reveal the significant role of the initial stage of peptide-membrane interaction and peptide structure in the mechanism of haemolysis. These data are not consistent with a lipid-based mechanism of peptide-induced haemolysis, indicating that the mode of peptide-protein interaction is an important and decisive step in the haemolytic mechanism. It should be noted that data (in the form of three additional Tables) on the ability of inhibitors to protect cells from haemolysis when inhibitor and peptide act simultaneously are available. They are reported in Supplementary Publication SUP 50178, which has been deposited at the British Library Document Supply Centre, Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1996) 313, 9.

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Cation-sensitive pore formation in rehydrated erythrocytes

Rudenko

Patelaros

1995

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Rehydration of red blood cells (RBC) in isotonic media after dehydration in hypertonic electrolyte or nonelectrolyte saline leads to their posthypertonic hemolysis (PH). Ca2+ ions at a concentration of more than 5 mM stimulated hemolysis of RBC treated by hypertonic sucrose but not NaCl if rehydration was carried out in the presence of cations. Zn2+ produced a more complex response of stimulation followed by inhibition as a concentration is increased. Mg2+, Ca2+, Zn2+, EDTA and sucrose exhibited only inhibition when added to isotonic NaCl media immediately after onset of rehydration or later on. At low ionic strength inhibition produced by divalent cations was markedly reduced and sucrose was ineffective. An equimolar concentration of EDTA abolished the inhibition of PH by Zn2+ ions if they were introduced into the isotonic media after the cells, but activated hemolysis when rehydration was carried out in the presence of ions. The same divalent cations prevented shape transformation and hemolysis induced by melittin if they interacted with the plasma membrane prior to the addition of melittin. Subsequent chelation of cations by EDTA triggers the full sequence of events characteristic to the action of melittin alone and resulted in cell spherulation followed by hemolysis. Inhibition of melittin-induced hemolysis produced by all cations was reversible because EDTA abolished the action of divalent cations and even stimulated hemolysis in isotonic sucrose. Similarities in the mode of action of divalent cations and EDTA on posthypertonic hemolysis which is attributed to endogenous stimuli and melittin-induced hemolysis as far as the exogenous agent is concerned imply that in both cases common intrinsic mechanisms are involved in the process of cation-sensitive pore formation in erythrocyte membranes, while differences indicate that more complex pores are formed during posthypertonic injury.

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Deformability and other rheological interactions of red blood cells in electronic cell sizing

Cited by 5 publications

References 0 publications

Erythrocyte lysis and angle‐resolved light scattering measured by scanning flow cytometry result to 48 indices quantifying a gas exchange function of the human organism

Erythrocyte lysis and angle‐resolved light scattering measured by scanning flow cytometry result to 48 indices quantifying a gas exchange function of the human organism

Protection by chlorpromazine, albumin and bivalent cations against haemolysis induced by melittin, [Ala-14]melittin and whole bee venom

Cation-sensitive pore formation in rehydrated erythrocytes

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