Deformation regimes of collagen fibrils in cortical bone revealed by in situ morphology and elastic modulus observations under mechanical loading

Yang, Pengfei; Nie, Xiao-Tong; Zhao, Dongdong; Wang, Zhe; Ren, Li; Xu, Huiyun; Rittweger, Jörn; Shang, Peng

doi:10.1016/j.jmbbm.2017.12.015

Cited by 14 publications

(18 citation statements)

References 38 publications

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“…In order to assess the changes of the MCFs in tibia from different groups to mechanical loading, a murine tibia loading device, which can be used for in situ AFM scanning, was custom-built, as described previously (Yang et al, 2018). A piezoelectric actuator (PK2FVF1, Thorlabs, NJ, United States) was used to generate the precise mechanical force on the tibia.…”

Section: Methodsmentioning

confidence: 99%

“…The preloading condition was referred to as the pre-load condition in the following text. Our previous study showed that the most dramatic changes of the MCFs are induced by the mechanical loading of 6 N (Yang et al, 2018). Therefore, pre-load and 6 N were chosen to load the tibia at each session of the AFM scanning.…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, five minutes rest time was given between the adjacent scans to let the bone recover and avoid the potential influence of previous loading on bone morphology at the level of the collagen fibrils. Previous finite-element analyses indicated that the scanning sites were under tension with the average strain ranging from 1000 to 1500 με (Yang et al, 2018). Digital Image Correlation approach (Guo et al, 2017) was adopted to quantify the surface strain of tibia from both the control and tail suspension groups.…”

Section: Methodsmentioning

confidence: 99%

“…While the tibia was fixed on the axial loading device, a relatively flat surface in the antero-medial aspect of the tibia was chosen for the AFM scan (six tibias from six different mice). The sample preparation, demineralization and AFM scanning procedure were described in our previous publication (Yang et al, 2018). In detail, the cortical bone surface of the chosen sites were polished using 7000 grit sand paper, 3-μm diamond suspension, and 0.5-μm diamond suspension in sequence.…”

Section: Methodsmentioning

confidence: 99%

“…Depends on the image quality, images of three bones chosen from six tibias were used for D-periodic spacing, fibril organization and radial elastic modulus calculations. The detailed procedure of AFM image analyses was also described below (Yang et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

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Disuse Impairs the Mechanical Competence of Bone by Regulating the Characterizations of Mineralized Collagen Fibrils in Cortical Bone

et al. 2019

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Bones are made of complex material comprising organic components and mineral hydroxyapatite, both of which formulate the unique hierarchical structure of bone and its mechanical properties. Bones are capable of optimizing their structure and mechanical properties according to the mechanical environment. Mineral loss is a well-known consequence of skeleton disuse. By contrast, the response of the non-mineral phase of bone, i.e., the collagen network, during disuse remain largely unknown. In this study, a tail-suspension mice model was used to induce bone loss. Atomic force microscopy-based imaging and indentation approaches were adopted to investigate the influence of disuse on the morphology and in situ mechanical behavior of the collagen fibrils, under both non-loaded and load-bearing conditions, in the cortical tibia of mice. The results indicate that disuse induced by hindlimb unloading did not alter the orientation and D-periodic spacing of the collagen fibril, but results in decreased collagen crosslinking which correlates with decreased elasticity and increased susceptibility to mechanical damage. More concretely, the collagen fibrils in the disused tibia were misaligned under mechanical loading. It therefore indicates that the disordered arrangement of the mineralized collagen fibrils is one of the characteristics of the weakened bone during elastic deformation. These findings reveals the unique adaptation regimes of the collagen fibrils in the cortical bone to disuse, as well as the deformation mechanisms of bone in the relevant pathological process at different scales.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Disuse Impairs the Mechanical Competence of Bone by Regulating the Characterizations of Mineralized Collagen Fibrils in Cortical Bone

et al. 2019

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Structural Orientation and Anisotropy in Biological Materials: Functional Designs and Mechanics

Liu

Zhang

Ritchie

2020

Adv Funct Materials

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Biological materials exhibit anisotropic characteristics because of the anisometric nature of their constituents and their preferred alignment within interfacial matrices. The regulation of structural orientations is the basis for material designs in nature and may offer inspiration for man‐made materials. Here, how structural orientation and anisotropy are designed into biological materials to achieve diverse functionalities is revisited. The orientation dependencies of differing mechanical properties are introduced based on a 2D composite model with wood and bone as examples; as such, anisotropic architectures and their roles in property optimization in biological systems are elucidated. Biological structural orientations are designed to achieve extrinsic toughening via complicated cracking paths, robust and releasable adhesion from anisotropic contact, programmable dynamic response by controlled expansion, enhanced contact damage resistance from varying orientations, and simultaneous optimization of multiple properties by adaptive structural reorientation. The underlying mechanics and material‐design principles that could be reproduced in man‐made systems are highlighted. Finally, the potential and challenges in developing a better understanding to implement such natural designs of structural orientation and anisotropy are discussed in light of current advances. The translation of these biological design principles can promote the creation of new synthetic materials with unprecedented properties and functionalities.

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Adaptive structural reorientation: Developing extraordinary mechanical properties by constrained flexibility in natural materials

Liu

Zhang

et al. 2019

Acta Biomaterialia

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Deformation regimes of collagen fibrils in cortical bone revealed by in situ morphology and elastic modulus observations under mechanical loading

Cited by 14 publications

References 38 publications

Disuse Impairs the Mechanical Competence of Bone by Regulating the Characterizations of Mineralized Collagen Fibrils in Cortical Bone

Disuse Impairs the Mechanical Competence of Bone by Regulating the Characterizations of Mineralized Collagen Fibrils in Cortical Bone

Structural Orientation and Anisotropy in Biological Materials: Functional Designs and Mechanics

Adaptive structural reorientation: Developing extraordinary mechanical properties by constrained flexibility in natural materials

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