Degeneration of cryopreserved bovine oocytes via apoptosis during subsequent culture

Men, Hongsheng; Monson, R.L.; Parrish, J.J.; Rutledge, J. J.

doi:10.1016/s0011-2240(03)00070-1

Cited by 95 publications

(54 citation statements)

References 42 publications

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“…Nevertheless, cleavage rates higher than 45% for oocytes vitrified both as immature and matured have been reported by other researchers [9,16]. In our work, blastocyst rates produced from vitrified, mature oocytes are lower than those presented in previous reports [5,9,10,15,16,35] and comparable to those published by Men et al [16] using immature oocytes. Live calves have been obtained from vitrified/ warmed immature oocytes [9].…”

Section: Discussionsupporting

confidence: 79%

“…Apoptosis [10,26,36,52] and disorganization of the MII spindle as a cause of chromosomal aberrations [31,52,53], have been observed in oocytes after vitrification procedures. In addition, ZP may be either broken or at least modified by the premature release of CG, as seen in frozen-thawed human and mouse oocytes [44,54].…”

Section: Discussionmentioning

confidence: 99%

“…Modification of the culture systems are worth to be tested in order to overcome the cryodamage inflicted to the oocyte [10].…”

Section: Discussionmentioning

confidence: 99%

“…This can be primarily explained by nuclear damage [10,11], including spindle disorganization and loss of clumping of microtubules [10,12,13], as well as other ultrastructural alterations seen in vitrified oocytes [14]. The nuclear stage at which oocytes are submitted to cryopreservation influences further ability of the oocyte to develop after in vitro fertilization (IVF) [15].…”

Section: Introductionmentioning

confidence: 99%

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Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability

et al. 2005

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The nuclear stage at which oocytes are cryopreserved influences further development ability and cryopreservation affects ultrastructure of both cumulus cells and the oocyte. In this work, we analyze the effects of vitrification at different nuclear and cytoplasmic maturation stages on the oocyte ultrastructure and developmental ability. Culture in TCM199 + PVA with roscovitine 25 M during 24 h led to meiotic arrest (MA) in cumulus-oocyte complexes (COCs), while permissive in vitro maturation (IVM) was performed in TCM199, 10% FCS, FSH-LH and 17b-estradiol for 24 h. Oocytes were vitrified using the open pulled straw method (OPS) with minor modifications. Fresh and vitrified/warmed COCs were fixed as immature, after IVM, after meiotic arrest (MA) and after MA + IVM.Vitrification combined with MA followed by IVM produced the highest rates of degeneration, regardless of the vitrification time. As a consequence, lower proportions of embryos cleaved in these groups, although differences were eliminated at the five-eight cell stage. Development rates up to day 8 were similar in all experimental groups, being significantly lower than those in fresh controls. Only oocytes vitrified after IVM were able to give blastociysts. The morphological alterations observed can be responsible for compromised development. More research is needed to explain the low survival rates of the bovine oocyte after vitrification and warming. #

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

“…Modification of the culture systems are worth to be tested in order to overcome the cryodamage inflicted to the oocyte [10].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability

et al. 2005

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“…However, this condition can be alleviated by using vitrification, but it must be considered that different factors in vitrification such as high cryoprotectant concentrations, cooling, and osmotic stress may contribute to the initiation of apoptosis that threaten later viability and development [16]. However, in the absence of cryopreservation, the process of in vitro maturation also induces degeneration via an apoptotic pathway [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes

Ebrahimi

Valojerdi

Eftekhari‐Yazdi

et al. 2010

J Assist Reprod Genet

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Purpose The purpose of this study was to evaluate the effects of cryotop vitrification of sheep cumulus-oocyte complexes (COCs) on oocyte maturation, apoptotic gene expression and incidence of chromosomal abnormalities. Methods Freshly isolated (control group) and vitrifiedwarmed COCs (cryotop group) were matured in vitro. The expression of pro-and anti-apoptotic genes was investigated by real-time PCR. The incidence of numerical chromosomal abnormalities was evaluated by cytogenetic analysis. Results The mean percentage of oocytes in the cryotop and control groups that reached metaphase II was 49.25±3.01% and 51.94±2.7% respectively. The expression rates of proand anti-apoptotic genes were similar in both groups, whereas the incidence of numerical chromosomal abnormalities was higher in the cryotop group compared to the control group (42.5% vs. 20%, p<0.05). Conclusion Although cryotop vitrification of COCs did not affect the incidence of oocyte maturation or apoptotic gene expression, significant deficiencies in the maintenance of oocyte chromosomal organization were seen.

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Maintaining Cultures

2010

Practical Cell Analysis

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Degeneration of cryopreserved bovine oocytes via apoptosis during subsequent culture

Cited by 95 publications

References 42 publications

Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability

Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability

In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes

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