Degradation and hemostatic properties of polyphosphate coacervates

Momeni, Arash; Filiaggi, Mark

doi:10.1016/j.actbio.2016.06.002

Cited by 32 publications

(27 citation statements)

References 37 publications

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“…From studies with Ca 2+ phosphate and carbonate nanoparticles it is known that the crystallization can pass through a gelly-like phase, the coacervate. 111,112 Such a phase is formed during an electrostatically-driven liquid-liquid transition phase, resulting from the association of oppositely charged (macro)-ions, the negatively charged polyP and the water layer. Those coacervate assemblies can measure 4100 mm and are formed from their soluble precursors of less than 200 nm.…”

Section: Polyphosphate Nano-/microparticlesmentioning

confidence: 99%

Amorphous polyphosphate, a smart bioinspired nano-/bio-material for bone and cartilage regeneration: towards a new paradigm in tissue engineering

2018

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Section: Polyphosphate Nano-/microparticlesmentioning

confidence: 99%

Amorphous polyphosphate, a smart bioinspired nano-/bio-material for bone and cartilage regeneration: towards a new paradigm in tissue engineering

2018

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“…In this study, we show that Ca‐polyP‐NP can be formed in situ within a KG:PVA copolymer cryogel where they retain their biological and morphogenetic properties. Like in the free state, the nanoparticles within the cryogel undergo coacervation after transfer of the material into a protein/peptide‐containing medium and by that become biochemically active . The material is a dynamic hydrogel with pronounced viscoelastic properties.…”

Section: Introductionmentioning

confidence: 99%

“…[20,39] The material is a dynamic hydrogel with pronounced viscoelastic properties. Like in the free state, the nanoparticles within the cryogel undergo coacervation after transfer of the material into a protein/peptide-containing medium and by that become biochemically active.…”

mentioning

confidence: 99%

In Situ Polyphosphate Nanoparticle Formation in Hybrid Poly(vinyl alcohol)/Karaya Gum Hydrogels: A Porous Scaffold Inducing Infiltration of Mesenchymal Stem Cells

et al. 2018

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The preparation and characterization of a porous hybrid cryogel based on the two organic polymers, poly(vinyl alcohol) (PVA) and karaya gum (KG), into which polyphosphate (polyP) nanoparticles have been incorporated, are described. The PVA/KG cryogel is prepared by intermolecular cross‐linking of PVA via freeze‐thawing and Ca2+‐mediated ionic gelation of KG to form stable salt bridges. The incorporation of polyP as amorphous nanoparticles with Ca2+ ions (Ca‐polyP‐NP) is achieved using an in situ approach. The polyP constituent does not significantly affect the viscoelastic properties of the PVA/KG cryogel that are comparable to natural soft tissue. The exposure of the Ca‐polyP‐NP within the cryogel to medium/serum allows the formation of a biologically active polyP coacervate/protein matrix that stimulates the growth of human mesenchymal stem cells in vitro and provides the cells a suitable matrix for infiltration superior to the polyP‐free cryogel. In vivo biocompatibility studies in rats reveal that already two to four weeks after implantation into muscle, the implant regions containing the polyP‐KG/PVA material become replaced by initial granulation tissue, whereas the controls are free of any cells. It is proposed that the polyP‐KG/PVA cryogel has the potential to become a promising implant material for soft tissue engineering/repair.

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“…The coagulation cascade involves 2 common pathways, namely, extrinsic pathway and intrinsic pathway. Prothrombin time measures extrinsic pathway, which records the time required for PPP to form a fibrin gel after adding tissue factor . To investigate the effect of Cs/Gel MPs on extrinsic pathway, PT assay was carried out.…”

Section: Resultsmentioning

confidence: 99%

Genipin crosslinked microspheres as an effective hemostatic agent

Xiao

et al. 2018

Polymers for Advanced Techs

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Efficient hemorrhage control is of extreme importance to decrease mortality rates arising from traumatic bleeding. However, traditional hemostatic agents are limited to unsatisfied biocompatibility, inconvenient usage, and high cost. To this end, we have devised a novel hemostatic microsphere from chitosan and gelatin by using appropriate amount of genipin as a crosslinker. The performance of these Cs/GelMPs was evaluated with experiments in vitro and in vivo. Results in vitro showed that the best hemostatic efficacy was achieved by Cs/Gel-0.75 MPs (the mass fraction of genipin in Cs/Gel mixture is 8.6 × 10 −6 ). Skin laceration model indicated that the hemostatic time of Cs/Gel-0.75 MPs was within 12 seconds, which has been significantly shortened in comparison with commercial available products. Moreover, cytocompatibility results displayed that Cs/Gel-0.75 MPs caused no obvious cytotoxicity even at the high concentration of 40 μg/ml. Cs/Gel-0.75 MPs also possess satisfactory fluid absorption ratio and low hemolysis. Therefore, these Cs/Gel-0.75 MPs are promising candidates for further surgical wound hemostatic agent.

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Degradation and hemostatic properties of polyphosphate coacervates

Cited by 32 publications

References 37 publications

Amorphous polyphosphate, a smart bioinspired nano-/bio-material for bone and cartilage regeneration: towards a new paradigm in tissue engineering

Amorphous polyphosphate, a smart bioinspired nano-/bio-material for bone and cartilage regeneration: towards a new paradigm in tissue engineering

In Situ Polyphosphate Nanoparticle Formation in Hybrid Poly(vinyl alcohol)/Karaya Gum Hydrogels: A Porous Scaffold Inducing Infiltration of Mesenchymal Stem Cells

Genipin crosslinked microspheres as an effective hemostatic agent

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