Degradation and Remodeling of Small Intestinal Submucosa in Canine Achilles Tendon Repair

Gilbert, Thomas W.; Stewart‐Akers, Ann M.; Simmons-Byrd, Abby; Badylak, Stephen F.

doi:10.2106/00004623-200703000-00020

Cited by 134 publications

(121 citation statements)

References 31 publications

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“…[17][18][19] Several studies have shown that SIS can provide a collagenous matrix to promote cell migration into the healing site to enhance revascularization and repair. [20][21][22][23][24] It is hypothesized that SIS enhance and promote the healing of the ACL. Instead of using the SIS as a load-bearing 10-layered bioscaffold, like in the rotator cuff repair, only a single layer of SIS was used to recruit sufficient healing cells to the hypocellular ACL.…”

Section: Introductionmentioning

confidence: 99%

Healing of the Goat Anterior Cruciate Ligament After a New Suture Repair Technique and Bioscaffold Treatment

Nguyen

Geel

Schulze

et al. 2013

Tissue Engineering Part A

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Primary suture repair of the anterior cruciate ligament (ACL) has been used clinically in an attempt to heal the ruptured ACL. The results, however, were not satisfactory, which in retrospect can be attributed to the used suturing technique and the suboptimal healing conditions. These constraining conditions can be improved by introducing a new suturing technique and by using small intestinal submucosa (SIS) as a bioscaffold. It is hypothesized that the suturing technique keep the torn ends together and that SIS enhance and promote the healing of the ACL. The goat was used as the study model. In the Suture group, the left ACL was transected and suture repaired with a new locking suture repair technique (n = 5) allowing approximation and fixation under tension. The Suture-SIS group underwent the same procedure with the addition of SIS (n = 5). The right ACL served as control. After 12 weeks of healing, anterior-posterior translation and in situ force of the healing ACL were measured, followed by the measurement of the cross-sectional area and structural stiffness. Routine histology was performed on tissue samples. Gross morphology showed that the healing ACL was continuous with collagenous tissue in both groups. The cross-sectional area of the Suture and the Suture-SIS group was 35% and 50% of the intact control, respectively. The anterior-posterior translations at different flexion angles were statistically not different between the Suture group and the Suture-SIS group. Only the in situ force at 30°in the Suture-SIS group was higher than in the Suture group. Tensile tests showed that the stiffness for the Suture group was not different from the Suture-SIS group (31.1 -8.1 N/mm vs. 41.9 -18.0 N/mm [p > 0.05]). Histology showed longitudinally aligned collagen fibers from origo to insertion. More fibroblasts were present in the healing tissue than in the control intact tissue. The study demonstrated the proof of concept of ACL repair in a goat model with a new suture technique and SIS. The mechanical outcome is not worse than previously reported for ACL reconstruction. In conclusion, the approach of using a new suture technique, with or without a bioscaffold to heal the ACL is promising.

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Section: Introductionmentioning

confidence: 99%

Healing of the Goat Anterior Cruciate Ligament After a New Suture Repair Technique and Bioscaffold Treatment

Nguyen

Geel

Schulze

et al. 2013

Tissue Engineering Part A

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“…Nonchemically cross-linked scaffolds have been shown to be resorbed into the body within 60 to 90 days. 10,11 The degradation of the scaffold unlocks properties not associated with intact ECM; for example, studies have shown collagen degraded by proteases to have regenerative capacity. 24 Degradation of the UBM was one of the earliest events observed after device placement.…”

Section: Discussionmentioning

confidence: 99%

“…On implantation, the ECM material degrades rapidly. 10,11 Rapid degradation is thought to be critical to the scaffold remodeling process. 12,13 In normal wound healing, the host ECM is vital as it acts as a support structure for cell migration.…”

Section: Introductionmentioning

confidence: 99%

The Clinical Effectiveness in Wound Healing With Extracellular Matrix Derived From Porcine Urinary Bladder Matrix: A Case Series on Severe Chronic Wounds

Kimmel

Rahn

Gilbert

2010

The Journal of the American College of Certified Wound Speciali

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A study involving 3 patients was performed to evaluate the efficacy of Matristem, an extracellular matrix scaffold derived from the porcine urinary bladder matrix (UBM), to promote natural healing in patients with severe, chronic wounds that did not respond well to standard wound management. In all cases, the wounds closed after only a few weeks of repeated treatments with UBM, with all patients showing complete epithelialization of the wound with limited formation of scar tissue by 13 weeks after first treatment. These initial results suggest that UBM warrants further investigation for treatment of chronic nonhealing ulcers that are recalcitrant to standard wound therapies.

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“…6 For samples that were grafted with MatriStem alone, the tissue contribution was equal to those values obtained for the entire complex. Since noncrosslinked ECM grafts degrade rapidly in vivo (10-ply 14 C-labeled ECM was completely degraded by 3 months), 21 we assumed that MatriStem grafts were completely degraded during the implantation period (3 months) and replaced by host connective tissue, and thus, the ECM graft did not contribute to the mechanical integrity at the time of tissue procurement.…”

Section: Surgical Proceduresmentioning

confidence: 99%

Extracellular Matrix Regenerative Graft Attenuates the Negative Impact of Polypropylene Prolapse Mesh on Vagina in Rhesus Macaque

Liang

Knight

Barone

et al. 2017

Obstet Gynecol Surv

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BACKGROUND-The use of wide pore lightweight polypropylene mesh to improve anatomical outcomes in the surgical repair of prolapse has been hampered by mesh complications. One of the prototype prolapse meshes has been found to negatively impact the vagina by inducing a decrease in smooth muscle volume and contractility and the degradation of key structural proteins (collagen and elastin), resulting in vaginal degeneration. Recently, bioscaffolds derived from extracellular matrix have been used to mediate tissue regeneration and have been widely adopted in tissue engineering applications.

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Degradation and Remodeling of Small Intestinal Submucosa in Canine Achilles Tendon Repair

Cited by 134 publications

References 31 publications

Healing of the Goat Anterior Cruciate Ligament After a New Suture Repair Technique and Bioscaffold Treatment

Healing of the Goat Anterior Cruciate Ligament After a New Suture Repair Technique and Bioscaffold Treatment

The Clinical Effectiveness in Wound Healing With Extracellular Matrix Derived From Porcine Urinary Bladder Matrix: A Case Series on Severe Chronic Wounds

Extracellular Matrix Regenerative Graft Attenuates the Negative Impact of Polypropylene Prolapse Mesh on Vagina in Rhesus Macaque

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