Degradation enhances the anticoagulant and antiplatelet activities of polysaccharides from Lycium barbarum L. leaves

Lin, Shenzhu; Al-Wraikat, Majida; Niu, Lin-Ru; Zhou, Fa; Zhang, Yanyan; Wang, Mengze; Ren, Jie; Fan, Jie; Zhang, Bolin; Wang, Liying

doi:10.1016/j.ijbiomac.2019.04.147

Cited by 47 publications

(18 citation statements)

References 25 publications

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“…AAAP-1 (47.3 s, 50.0 µg/mL) prolonged the clotting time of APTT significantly compared to the saline control (28.0 s), but it was much lower than that of heparin (48.7 s, 2.0 µg/mL). This result is very close to the studies of Li, C. et al [54] and Lin, S. et al [55], and represented a deficiency in factors VIII, IX, XI, XII, or Von Willebrand's factor (VWF). AAAP-1 in vitro (12.5-50 µg/mL) could prolong PT (16.1-73.7 s) and TT (20.3-28.2 s) significantly (p < 0.01) compared to the saline control (11.3 and 17.7 s).…”

Section: Anticoagulant Activity Of Aaap-1 In Vitrosupporting

confidence: 91%

Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Acidic Polysaccharides from Auricularia auricula-judae

2020

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To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM). The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W). Furthermore, the acidic AAP (aAAP) was precipitated with cetyltrimethylammonium bromide (CTAB) from crude AAP (cAAP). aAAP was further purified using ion exchange chromatography with a DEAE Purose 6 Fast Flow column to obtain aAAP-1. Additionally, according to the HPLC analysis, the aAAP-1 was mainly composed of mannose, glucuronic acid, glucose, galactose, and xylose, with a molar ratio of 80.63:9.88:2.25:1:31.13. Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.

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Section: Anticoagulant Activity Of Aaap-1 In Vitrosupporting

confidence: 91%

Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Acidic Polysaccharides from Auricularia auricula-judae

2020

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“…Recently, it has been reported that L. barbarum leaves contain a polysaccharide–protein complex, rich in carbohydrates (including arabinose, galactose, glucose, mannose, rhamnose, ribose, and xylose), uronic acid, and calcium. This complex exhibits interesting health properties, among which are anticoagulant and antiplatelet activities [14]. As regards phenol compounds, the most abundant is rutin (quercetin-3- O -rutinoside), but also chlorogenic acid and scopoletin have been reported.…”

Section: Introductionmentioning

confidence: 99%

Phenol Profiling and Nutraceutical Potential of Lycium spp. Leaf Extracts Obtained with Ultrasound and Microwave Assisted Techniques

et al. 2019

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In recent years, agricultural and industrial residues have attracted a lot of interest in the recovery of phytochemicals used in the food, pharmaceutical, and cosmetic industries. In this paper, a study on the recovery of phenol compounds from Lycium spp. leaves is presented. Ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) have been used with alcoholic and hydroalcoholic solvents. Methanolic UAE was the most successful technique for extracting phenols from Lycium leaves, and we used on leaves from L. barbarum and L. chinense cultivated in Italy. The extracts were then characterized as regards to the antioxidant properties by in vitro assays and the phenol profiling by a high performance liquid chromatography-diode array detector (HPLC-DAD). Chlorogenic acid and rutin were the main phenol compounds, but considerable differences have been observed between the samples of the two Lycium species. For example, cryptochlorogenic acid was found only in L. barbarum samples, while quercetin-3-O-rutinoside-7-O-glucoside and quercetin-3-O-sophoroside-7-O-rhamnoside only in L. chinense leaves. Finally, multivariate statistical analysis techniques applied to the phenol content allowed us to differentiate samples from different Lycium spp. The results of this study confirm that the extraction is a crucial step in the analytical procedure and show that Lycium leaves represent an interesting source of antioxidant compounds, with potential use in the nutraceutical field.

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“…The LBLP was estimated to be 16.21% of the dry weight (Liu et al ., 2012). A few LBLP with molecular weights (MW) ranging in 46.3–418 kDa have been isolated and purified from aqueous extracts of LBL by DEAE cellulose typically (Liu et al ., 2012; Lin et al ., 2019). For identification of the structure, analytic methods, including gas chromatography (GC), high‐performance gel‐permeation chromatography (HPGPC), nuclear magnetic resonance (NMR) and gas chromatography‐mass spectrometry (GC–MS) have been used; the results were summarised in Table S1.…”

Section: Phytochemicals In Goji Leavementioning

confidence: 99%

Phytochemicals and bioactivities of Goji (Lycium barbarum L. and Lycium chinense Mill.) leaves and their potential applications in the food industry: a review

Lei

Chen

Cao

et al. 2021

Int J of Food Sci Tech

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Goji leaves, including Lycium barbarum L. and Lycium chinense Mill. leaves, have been used as traditional Chinese medicine materials for nourishing kidneys and enhancing eyesight but are mostly discarded at present. The nutritional composition of Goji leaves is similar to that of Goji berries even some components are more plentiful in the former, such as flavonoids. This paper firstly reviews the main phytochemicals of Goji leaves. Also, updated information on the biological activities of Goji leaves is narrated. Based on the long use history and existing bioactivity data on Goji leaves, we propose their potential applications in the food industry and encourage further investigation on Goji leaves.

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Degradation enhances the anticoagulant and antiplatelet activities of polysaccharides from Lycium barbarum L. leaves

Cited by 47 publications

References 25 publications

Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Acidic Polysaccharides from Auricularia auricula-judae

Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Acidic Polysaccharides from Auricularia auricula-judae

Phenol Profiling and Nutraceutical Potential of Lycium spp. Leaf Extracts Obtained with Ultrasound and Microwave Assisted Techniques

Phytochemicals and bioactivities of Goji (Lycium barbarum L. and Lycium chinense Mill.) leaves and their potential applications in the food industry: a review

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