Degradation kinetics of toilet paper fiber during wastewater treatment: Effects of solid retention time and microbial community

Li, Simeng; Wu, Zhuangzhuang; Liu, Guoqiang

doi:10.1016/j.chemosphere.2019.03.097

Cited by 29 publications

(10 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The diluted DNA was used as the template for PCR amplification (26 cycles) of bacterial 16 S rRNA genes with barcoded primers and HiFi Hot Start Ready Mix (KAPA). For the bacterial diversity analysis, the V3-V4 variable region of the 16 S rRNA genes was amplified with universal primers (343 F 5′-TACGGRAGGCAGCAG-3′, 798 R 5′-AGGGTATCTAATCCT-3′) 83 . The length of the PCR product was about 445 bp.…”

Section: Cell Viability Analysis Of the Dust And Filter Samples The mentioning

confidence: 99%

Bacterial community analysis of floor dust and HEPA filters in air purifiers used in office rooms in ILAS, Beijing

Guo

Xiong

Kang

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Air purifiers with high-efficiency particulate air (HEPA) filters remove not only particulate matter but also airborne microorganisms in indoor environments. We investigated the bacterial community in HEPA filters (used for 1 year) and that in the floor dust of 12 office rooms in Beijing. We found that the viable bacteria proportion in the filter was significantly higher than that in the floor dust (p < 0.001). The Non-Metric Multi-Dimensional Scaling analysis showed that the bacterial communities in the filters and dust were significantly different (p = 0.001). The Chao1, Shannon-Wiener and phylogenetic diversity values in the filter were significantly higher than those in the dust (p < 0.001). The predominant bacterial classes in the filter were Alphaproteobacteria and Actinobacteria, whereas those in the dust were Bacteroidia, Clostridia and Bacilli. Human occupancy contributed more to the bacterial community in the filter than that in the dust. Klebsiella and Alloprevotella in the dust and filters positively correlated with the occupancy density. Soil bacteria contributed to a significantly higher proportion of the bacteria in the HEPA filter (p < 0.001). In contrast, human oral, indoor air and outdoor haze contributed to a higher proportion of the bacteria in the dust samples (p < 0.001, p < 0.01 and p < 0.05, respectively). As HEPA filters serve as an ecological niche for indoor bacteria, they should be carefully investigated during the assessment of indoor environmental health. People spend the majority of their time indoors, wherein air pollution levels highly correlate with outdoor pollution levels 1-6. In recent years, haze pollution, fine particulate matter (PM) in particular, has been reported to increase human mortality rates owing to the development of heart disease 7,8 , stroke, respiratory disease 9 and lung cancer 10,11. The level of air pollution in China is substantially higher than in many developed countries 12. Moreover, its threat to public health has become a serious concern, particularly concerning haze events 13. Thus, an effective technology is essential for the removal of PM from indoor environments during periods of haze. To this end, air purifiers with high-efficiency particulate air (HEPA) filters are widely used in the indoor environment, particularly in areas with heavy haze 14. People are exposed to various microbes in indoor environments. Many factors could affect indoor microbial composition, including human occupancy, occupant behavior, pets and outdoor environment 15-18. The air purifier was mainly used to remove indoor PM, partly comprising microorganisms. Microbes in the air could be transmitted to the HEPA filter in the air purifier. Suzuki et al. (2003) collected swab samples from the air purifier filters in the houses of seven healthy children with varicella. They found the varicella-zoster virus was detected in 29% of filters in air purifiers by day 3 19. Furthermore, Maus et al. (2001) studied the effect of the filters on the viability of microbes. They reported microb...

show abstract

Section: Cell Viability Analysis Of the Dust And Filter Samples The mentioning

confidence: 99%

Bacterial community analysis of floor dust and HEPA filters in air purifiers used in office rooms in ILAS, Beijing

Guo

Xiong

Kang

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The high abundance of cellulose-degrading bacteria found in our study play an important role on fiber decomposition. The fiber decomposition compound can accumulate and hazardous impact the other steps such as sludge production and biogas production during the anaerobic processes [ 34 ]. Moreover, the predominance of Firmicutes in the SP1 tanks indicate a dependence on molecules produced by other groups of microorganisms once these syntrophic bacteria are capable of degrading cellulosic compounds and producing volatile fatty acids (VFAs) and H 2 , which are then consumed by hydrogenotrophic methanogens in metabolic reactions [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

On-Site Blackwater Treatment Fosters Microbial Groups and Functions to Efficiently and Robustly Recover Carbon and Nutrients

et al. 2020

View full text Add to dashboard Cite

Wastewater is considered a renewable resource water and energy. An advantage of decentralized sanitation systems is the separation of the blackwater (BW) stream, contaminated with human pathogens, from the remaining household water. However, the composition and functions of the microbial community in BW are not known. In this study, we used shotgun metagenomics to assess the dynamics of microbial community structure and function throughout a new BW anaerobic digestion system installed at The Netherlands Institute of Ecology. Samples from the influent (BW), primary effluent (anaerobic digested BW), sludge and final effluent of the pilot upflow anaerobic sludge blanket (UASB) reactor and microalgae pilot tubular photobioreactor (PBR) were analyzed. Our results showed a decrease in microbial richness and diversity followed by a decrease in functional complexity and co-occurrence along the different modules of the bioreactor. The microbial diversity and function decrease were reflected both changes in substrate composition and wash conditions. Our wastewater treatment system also decreased microbial functions related to pathogenesis. In summary, the new sanitation system studied here fosters microbial groups and functions that allow the system to efficiently and robustly recover carbon and nutrients while reducing pathogenic groups, ultimately generating a final effluent safe for discharge and reuse.

show abstract

“…The high abundance of cellulose-degrading bacteria found in our study play an important role on ber decomposition. The ber decomposition compound can accumulate and hazardous impact the other steps such as sludge production and biogas production during the anaerobic processes [22]. Moreover, the predominance of Firmicutes in the SP1 tanks indicate a dependence on molecules produced by other groups of microorganisms once these syntrophic bacteria are capable of degrading cellulosic compounds and producing volatile fatty acids (VFAs) and H 2 , which are then consumed by hydrogenotrophic methanogens in metabolic reactions [23].…”

Section: Discussionmentioning

confidence: 99%

On-Site Blackwater Treatment Fosters Microbial Groups and Functions to Efficiently and Robustly Recover Carbon and Nutrients

Kuramae

Dimitrov

Silva

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Wastewater is considered as a renewable resource water and energy. An advantage of decentralized sanitation systems is the separation of the blackwater (BW) stream, which is highly contaminated with human pathogens, from the remaining household water. However, the composition and functions of the microbial community in BW are not known. In this study, we used shotgun metagenomics to assess the dynamics of microbial community structure and function throughout a new BW anaerobic digestion system installed at The Netherlands Institute of Ecology. Samples from the influent (BW), primary effluent (anaerobic digested BW), sludge and final effluent of the pilot upflow anaerobic sludge blanket (UASB) reactor and microalgae pilot tubular photobioreactor (PBR) were analyzed.Results: Our results showed a decrease in microbial richness and diversity followed by a decrease in functional complexity and co-occurrence along the different modules of the bioreactor. The microbial diversity and function decrease were reflected both changes in substrate composition and wash conditions. The most prevalent core functions in influent (BW) were related to metabolism of carbohydrates, response to chemicals and drugs, and nitrogen. The core functions in anaerobic digested BW and upflow anaerobic sludge blanket reactor were related to response to stress, viral processes and iron-sulfur metabolism. Methanogenesis-related functions were most abundant in upflow anaerobic sludge blanket reactor. Effluent from tubular photobioreactor presented high abundances of functions related to nitrogen utilization, metal ion binding and antibiotic biosynthetic processes. Interestingly, the abundance of sequences related to ‘pathogenesis’ decreased from influent BW to SP1 to effluent from tubular photobioreactor. Our wastewater treatment system also decreased potential microbial functions related to pathogenesis. Conclusions: The new sanitation system studied here fosters microbial groups and functions that allow the system to efficiently and robustly recover carbon and nutrients while reducing pathogenic groups, ultimately generating a final effluent safe for discharge and reuse.

show abstract

Degradation kinetics of toilet paper fiber during wastewater treatment: Effects of solid retention time and microbial community

Cited by 29 publications

References 31 publications

Bacterial community analysis of floor dust and HEPA filters in air purifiers used in office rooms in ILAS, Beijing

Bacterial community analysis of floor dust and HEPA filters in air purifiers used in office rooms in ILAS, Beijing

On-Site Blackwater Treatment Fosters Microbial Groups and Functions to Efficiently and Robustly Recover Carbon and Nutrients

On-Site Blackwater Treatment Fosters Microbial Groups and Functions to Efficiently and Robustly Recover Carbon and Nutrients

Contact Info

Product

Resources

About