Degradation of Agro-Waste by Cellulase from Aspergillus candidus

Milala, Mohammed Adamu; Shehu, Binta Baba; Zanna, Hassan; Omosioda, V. O.

doi:10.3923/ajbkr.2009.51.56

Cited by 26 publications

(7 citation statements)

References 11 publications

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“…It seemed likely that the cell machinery of the organism during this period is directed towards active vegetative growth and mycelium proliferation. This result is in relation with the findings of Milala et al [59] for cellulase production by A. candidus. In this concern, Badhan et al [60] reported the highest FP-ase and CMC-ase that represent total cellulase activity on the 8 th and 10 th days of fermentation (410.76 and 480.48 U/g ds, respectively) and thereafter, the activity declined.…”

Section: Effect Of Different Incubation Periods On Cellulases Productionsupporting

confidence: 93%

Optimization of Culture Conditions for the Production of Extracellular Cellulases via Solid State Fermentation

El-Ghonemy

2014

BMRJ

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Aim:The aims of the present study were to screen different filamentous fungi for extracellular cellulases production and to optimize solid-state fermentation medium and culture conditions to enhance cellulases production. Study Design: Using agro-industrial waste as raw material for the production of cellulases by a hyper cellulase producing fungus and evaluating the influence of various parameters to design a suitable SSF process for cellulase production.

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Section: Effect Of Different Incubation Periods On Cellulases Productionsupporting

confidence: 93%

Optimization of Culture Conditions for the Production of Extracellular Cellulases via Solid State Fermentation

El-Ghonemy

2014

BMRJ

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“…Also, many of filamentous fungi such as Aspergillus and Trichoderma spp. are well known as efficient producers of cellulases ( Milala et al, 2009;Immanuel et al, 2006 andCianchetta et al, 2010). Reddy et al (2014) isolated fungal isolates having cellulolytic activities including A. niger, A. flavus, Trichoderma sp., Penicillium sp., Acremonium sp.…”

Section: Qualitative Screening For Cellulolytic Activity Of Fungimentioning

confidence: 99%

“…A5, A6 and A7 proved to be the best CMCase and β-Glucosidase producers on both RS and SCB, as their CMCase ranged 6.3-7.9 U.ml -1 on RS (LSD=0.412) and 5.7-6.3 U.ml -1 on SCB (LSD=0.317), while their βglucosidase ranged 2.0-2.9 U.ml -1 on both RS (LSD=0.111) and SCB (LSD=0.098), respectively. In this regard, Milala et al (2009) demonstrated that filamentous fungi particularly Aspergillus spp. were well known as efficient producer of cellulases and reported A. niger as higher producer upon growth on cellulosic substrates.…”

Section: Quantitative Screening For Cellulases and Xylanase Productiomentioning

confidence: 99%

Multi fungal cellulases produced on pretreated sugar cane bagasse and rice straw cooperative actions

Mahdy

Emara

El-Salam

et al. 2018

Journal of Environmental Sciences. Mansoura University

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Twenty eight pre-identified fungal isolates and strains were tested qualitatively for their cellulolytic activities, among which 12 superior producers were quantitatively tested. Three Aspergillus spp. namely A5, A6 and A7 were found to be the best cellulolytic fungi, while both Aspergillus terreus NRRL260 and Phanerochaete chrysosporium NRRL6361 were found to be the best xylanase producers. On solid state fermentation (SSF) using pretreated rice straw (RS), the Aspergillus spp. A5, A6 and A7 gave total cellulases (as FPase), exocellulases (as CMCase) and cellobiase (as β-glucosidase) activities of ≥ 16, 130 and 21U.g-1 ds, respectively. On the other hand, Aspergillus terreus NRRL260 showed maximum xylanases activity of 804 U.g-1 ds. The recovered crude enzymes forming the crude enzyme mixture (CEM) was concentrated (LCEM), ammonium sulfate fractionated (PPE1), were semi-purified by gel permeation on Sephadex G100 and finally lyophilized to powder form (PPE2). The resulting cellulases specific activities were 0.50, 0.68, 1.01 and 1.47 U.mg-1 , respectively. Those purification and concentration steps resulted in purification fold increase by 1.28, 2.08 and 2.78 for LCEM, PPE1 and PPE2, respectively. PPE2 achieved maximum degree of saccharification (DoS) at enzyme load ≥ 10 FPU, substrate concentration ≥ 5% and incubation period after 48 hr to be 85%, 87% and 88%, respectively.

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“…Members of the section Candidi produce many industrially important enzymes and secondary metabolites with a biotechnological potential. For example, they produce extracellular lipases, xylanases and cellulases which have potential to be used in the waste degradation processes ( Milala et al 2009 , Garai & Kumar 2013 , Farias et al 2015 ) or metabolites with antioxidant activity which can be used in the process of maturing foods ( Grazia et al 1986 , Pitt & Hocking 1997 , Sunesen & Stahnke 2003 ). The production of bioactive metabolites with pharmacological potential has been identified in some species, for example chlorflavonin with antibiotic and antifungal effects ( Munden et al 1970 , Marchelli & Vining 1973 , Hubka et al 2014 ), compounds with significant anti-oxidative activity such as candidusin B, 3-hydroxyterphenyllin or dihydroxymethyl pyranone ( Yen et al 2001 , Elaasser et al 2011 ), cytotoxic candidusin A, preussin or p -terphenyl derivatives ( Kobayashi et al 1982 , Malhão et al 2019 , Lin et al 2021 ) and many other recently discovered compounds with antitumor, antimicrobial, cytotoxic or antiviral activities such as ascandinines ( Zhou et al 2021 ), taichunines ( El-Desoky et al 2021 ), unguisins ( Li et al 2020 ) or other p -terphenyl derivatives ( Han et al 2020 , Shan et al 2020 , Wang et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

A monograph of Aspergillus section Candidi

Glässnerová

Sklenář

Jurjević³

et al. 2022

Studies in Mycology

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Aspergillus section Candidi encompasses white- or yellow-sporulating species mostly isolated from indoor and cave environments, food, feed, clinical material, soil and dung. Their identification is non-trivial due to largely uniform morphology. This study aims to re-evaluate the species boundaries in the section Candidi and present an overview of all existing species along with information on their ecology. For the analyses, we assembled a set of 113 strains with diverse origin. For the molecular analyses, we used DNA sequences of three house-keeping genes (benA, CaM and RPB2) and employed species delimitation methods based on a multispecies coalescent model. Classical phylogenetic methods and genealogical concordance phylogenetic species recognition (GCPSR) approaches were used for comparison. Phenotypic studies involved comparisons of macromorphology on four cultivation media, seven micromorphological characters and growth at temperatures ranging from 10 to 45 °C. Based on the integrative approach comprising four criteria (phylogenetic and phenotypic), all currently accepted species gained support, while two new species are proposed (A. magnus and A. tenebricus). In addition, we proposed the new name A. neotritici to replace an invalidly described A. tritici. The revised section Candidi now encompasses nine species, some of which manifest a high level of intraspecific genetic and/or phenotypic variability (e.g., A. subalbidus and A. campestris) while others are more uniform (e.g., A. candidus or A. pragensis). The growth rates on different media and at different temperatures, colony colours, production of soluble pigments, stipe dimensions and vesicle diameters contributed the most to the phenotypic species differentiation.

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Degradation of Agro-Waste by Cellulase from Aspergillus candidus

Cited by 26 publications

References 11 publications

Optimization of Culture Conditions for the Production of Extracellular Cellulases via Solid State Fermentation

Optimization of Culture Conditions for the Production of Extracellular Cellulases via Solid State Fermentation

Multi fungal cellulases produced on pretreated sugar cane bagasse and rice straw cooperative actions

A monograph of Aspergillus section Candidi

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