Degradation of cell wall materials from sweetpotato, cassava, and potato by a bacterial protopectinase and terminal sugar analysis of the resulting solubilized products

Analysis of sugars in bee pollen and propolis by ligand exchange chromatography in combination with pulsed amperometric detection and mass spectrometry

“…In recent years, the use of APS columns has been largely superseded by a highly sensitive method for the analysis of sugars using the combination of ion exchange chromatography based on Carbopac columns in combination with pulsed amperometric detection which is sensitive to low picomoles of sugars. There are many examples in the literature regarding the application of this technique to analysis of sugars in plants and plant-related materials (Cordella et al, 2005;Guignard et al, 2005;Hanko and Rohrer, 2000;Kerherve´et al, 1995;Salvador et al, 2002). In this methodology, dilute sodium hydroxide solution is used as the eluent and a HPLC system which is resistant to sodium hydroxide has been used.…”

Determination of glucosamine and lactose in milk-based formulae by high-performance liquid chromatography

“…The classic method for the determination of saccharides is chemical analysis as well as the enzyme-catalyzed method and spectrophotometry (Brereton et al, 2003;Gonza´les et al, 2003;Salvador et al, 2002). The major disadvantage of these three methods consists in the difficulty of evaluating different saccharides simultaneously.…”