Degradation of corn stover by fungal cellulase cocktail for production of polyhydroxyalkanoates by moderate halophile Paracoccus sp. LL1

Sawant, Shailesh S.; Salunke, Bipinchandra K.; Kim, Beom Soo

doi:10.1016/j.biortech.2015.07.019

Cited by 81 publications

(25 citation statements)

References 31 publications

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“…The obtained results were also comparable to those obtained with Paracoccus sp. LL1 grown on corn stover hydrolysate (using the enzyme cellulase) containing 20 g/L sugar, which resulted in 7.18 ± 0.1 g/L CDW with 68.9 ± 1.6% (w/w) PHB [ 4 ].…”

Section: Resultsmentioning

confidence: 99%

“…Cellulosic resources include: agricultural and forest residues, as well as municipal and industrial waste products, which are represented as low-value renewable materials that offer a feasible option for the production of high-value-added products [ 3 ]. Several studies have investigated the production of PHAs from cellulose biomass hydrolysate by a variety of microorganisms, including recombinant Escherichia coli [ 4 – 12 ]. The direct use of hydrolysates of hemicelluloses as a mixture of sugars for PHA production without the removal of inhibitors has also been explored.…”

Section: Introductionmentioning

confidence: 99%

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Use of agro-industrial residue from the canned pineapple industry for polyhydroxybutyrate production by Cupriavidus necator strain A-04

Sukruansuwan

Napathorn

2018

Biotechnol Biofuels

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BackgroundPineapple is the third most important tropical fruit produced worldwide, and approximately 24.8 million tons of this fruit are produced annually throughout the world, including in Thailand, which is the fourth largest pineapple producer in the world. Pineapple wastes (peel and core) are generated in a large amount equal to approximately 59.36% based on raw material. In general, the anaerobic digestion of pineapple wastes is associated with a high biochemical oxygen demand and high chemical oxygen demand, and this process generates methane and can cause greenhouse gas emissions if good waste management practices are not enforced. This study aims to fill the research gap by examining the feasibility of pineapple wastes for promoting the high-value-added production of biodegradable polyhydroxybutyrate (PHB) from the available domestic raw materials. The objective of this study was to use agro-industrial residue from the canned pineapple industry for biodegradable PHB production.ResultsThe results indicated that pretreatment with an alkaline reagent is not necessary. Pineapple core was sized to − 20/+ 40 mesh particle and then hydrolyzed with 1.5% (v/v) H2SO4 produced the highest concentration of fermentable sugars, equal to 0.81 g/g dry pineapple core, whereas pineapple core with a + 20 mesh particle size and hydrolyzed with 1.5% (v/v) H3PO4 yielded the highest concentration of PHB substrates (57.2 ± 1.0 g/L). The production of PHB from core hydrolysate totaled 35.6 ± 0.1% (w/w) PHB content and 5.88 ± 0.25 g/L cell dry weight. The use of crude aqueous extract (CAE) of pineapple waste products (peel and core) as a culture medium was investigated. CAE showed very promising results, producing the highest PHB content of 60.00 ± 0.5% (w/w), a cell dry weight of 13.6 ± 0.2 g/L, a yield () of 0.45 g PHB/g PHB substrate, and a productivity of 0.160 g/(L h).ConclusionsThis study demonstrated the feasibility of utilizing pineapple waste products from the canned pineapple industry as lignocellulosic feedstocks for PHB production. C. necator strain A-04 was able to grow on various sugars and tolerate levulinic acid and 5-hydroxymethyl furfural, and a detoxification step was not required prior to the conversion of cellulose hydrolysate to PHB. In addition to acid hydrolysis, CAE was identified as a potential carbon source and offers a novel method for the low-cost production of PHB from a realistic lignocellulosic biomass feedstock.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Use of agro-industrial residue from the canned pineapple industry for polyhydroxybutyrate production by Cupriavidus necator strain A-04

Sukruansuwan

Napathorn

2018

Biotechnol Biofuels

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“…; Sawant et al . ). It should be considered that these growth experiments represent a first comprehensive attempt to study the kinetics of PHB production in Halomonas sp.…”

Section: Discussionmentioning

confidence: 97%

Polyhydroxybutyrate production by an extremely halotolerant Halomonas elongata strain isolated from the hypersaline meromictic Fără Fund Lake (Transylvanian Basin, Romania)

et al. 2018

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The tested strain showed the ability for significant production of natural, biodegradable polymers under nutrient limitation and hypersaline conditions suggesting its potentiality for further metabolic and molecular investigations towards enhanced biopolymer production. Additionally, this study reports on the unprecedented use of Raman and XPRD techniques to investigate PHAs of an extremely halotolerant bacterium, thus expanding the repertoire of physical methods to study green plastics derived from extremophilic microorganisms.

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“…CCB-MM3 [107] Paracoccus Paracoccus sp. LL1 [75] 3HV in glucose medium supplemented with valeric acid [53]. Similarly, Halomonas profundus produces PHBV with different molar fractions of 3HV in the presence of valeric acid and propionic acid [54].…”

Section: Genus Species Referencesmentioning

confidence: 99%

“…Using wheat bran hydrolysate and digested potato waste, H. boliviensis accumulated 43% (wt) PHB [49]. In a study conducted by Sawant et al, pre-treated corn stover was enzymatically hydrolysed by crude cellulase obtained from co-culture of Trichoderma reesei and Aspergillus niger [75]. The obtained hydrolysate mainly contained glucose, xylose, and cellobiose.…”

Section: Low-cost Substrate Usage By Halophilic Bacteriamentioning

confidence: 99%

Current developments on polyhydroxyalkanoates synthesis by using halophiles as a promising cell factory

et al. 2020

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Plastic pollution is a severe threat to our environment which necessitates implementation of bioplastics to realize sustainable development for a green world. Polyhydroxyalkanoates (PHA) represent one of the potential candidates for these bioplastics. However, a major challenge faced by PHA is the high production cost which limits its commercial application. Halophiles are considered to be a promising cell factory for PHA synthesis due to its several unique characteristics including high salinity requirement preventing microbial contamination, high intracellular osmotic pressure allowing easy cell lysis for PHA recovery, and capability to utilize wide spectrum of low-cost substrates. Optimization of fermentation parameters has made it plausible to achieve large-scale production at low cost by using halophiles. Further deeper insights into halophiles have revealed the existence of diversified and even novel PHA synthetic pathways within different halophilic species that greatly affects PHA type. Thus, precise metabolic engineering of halophiles with the help of advanced tools and strategies have led to more efficient microbial cell factory for PHA production. This review is an endeavour to summarize the various research achievements in these areas which will help the readers to understand the current developments as well as the future efforts in PHA research.

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Degradation of corn stover by fungal cellulase cocktail for production of polyhydroxyalkanoates by moderate halophile Paracoccus sp. LL1

Cited by 81 publications

References 31 publications

Use of agro-industrial residue from the canned pineapple industry for polyhydroxybutyrate production by Cupriavidus necator strain A-04

Use of agro-industrial residue from the canned pineapple industry for polyhydroxybutyrate production by Cupriavidus necator strain A-04

Polyhydroxybutyrate production by an extremely halotolerant Halomonas elongata strain isolated from the hypersaline meromictic Fără Fund Lake (Transylvanian Basin, Romania)

Current developments on polyhydroxyalkanoates synthesis by using halophiles as a promising cell factory

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