Degradation of labeled propionic and acetic acids

Phares, E.F.

doi:10.1016/0003-9861(51)90094-x

Cited by 390 publications

(69 citation statements)

References 3 publications

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“…There was a marked difference in the responses to increasing levels of the two cations. Growth was stimulated maximally by the addition of 20 ble of utilizing aminoalkylphosphonic acids as phosphorus sources for growth (8,22). Three aminoalkylphosphonic acids (2-amino-3-phosphonopropionic acid, 1-aminoethylphosphonic acid, and 2-aminoethylphosphonic acid) were inactive as phosphorus sources for growth of S. fradiae.…”

Section: Resultsmentioning

confidence: 99%

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Biosynthesis of Fosfomycin by Streptomyces fradiae

Rogers

Birnbaum

1974

Antimicrob Agents Chemother

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The antibiotic fosfomycin was produced as a secondary metabolite in a glucose-asparagine medium containing citrate, l -methionine, and l -glutamate. The citrate requirement for antibiotic synthesis was related to its requirement for growth. In contrast, l -methionine and l -glutamate caused a marked stimulation of fosfomycin production and had no effect on growth. l -Methionine had to be added early to effect maximal antibiotic synthesis later in the fermentation. The l -glutamate requirement was not specific, since several tricarboxylic acid cycle intermediates could replace this amino acid. l -Asparagine was the most effective nitrogen source for growth and production of fosfomycin. Glycine, an alternate nitrogen source, supported fosfomycin synthesis only when added in excess of that needed for growth. Cobalt and inorganic phosphate were required also for antibiotic production at concentrations exceeding those supporting maximal growth. Radioactive incorporation studies showed that the methyl carbon of methionine was the precursor of the methyl of fosfomycin. Carbon 1 of fosfomycin was derived from glucose carbons 1 and 6, whereas glucose- 2 - 14 C labeled fosfomycin carbon 2. Radioactivity from acetate- 2 - 14 C was distributed equally between fosfomycin carbons 1 and 2. No incorporation of acetate- 1 - 14 C , asparagine- U - 14 C , citrate- 1,5 - 14 C , or glutamate- U - 14 C occurred. The labeling pattern of fosfomycin carbons 1 and 2 was similar to that found in 2-aminoethylphosphonate from Tetrahymena .

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Section: Resultsmentioning

confidence: 99%

“…Acetate obtained in this manner was degraded to its respective carbon atoms. Carbon 1 of acetate (represents carbon 2 of fosfomycin) was obtained by the Schmidt reaction (34) as described by Phares (20). One millimole of sodium acetate was degraded, and the resulting CO, was trapped in CO,-free 2 N NaOH.…”

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confidence: 99%

Biosynthesis of Fosfomycin by Streptomyces fradiae

Rogers

Birnbaum

1974

Antimicrob Agents Chemother

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“…When alcohol was collected it was converted to acetate as described earlier (2). The acetate was then divided into two equal samples, one of which was used for a total oxidation (13) and the other for the determination of radioactivity in the COOH group by the Schmidt reaction (12). The total activity in the CH3 group was then calculated by difference.…”

Section: Methodsmentioning

confidence: 99%

Intermediates of the Pentose Phosphate Pathway as Respiratory Substrates.

Beevers¹

1956

Plant Physiol.

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“…An aliquot of the combined extracts of fatty acids was decarboxylated according to the method of Phares [34] as modified by Goldfine and Bloch 1351. A known amount of [l-14C]stearic acid or [2-14C]-stearic acid was decarboxylated under identical conditions as a control.…”

Section: Decarboxylation Of Fatty Acidsmentioning

confidence: 99%

Fatty‐Acid Biosynthesis

Landriscina¹,

Gnoni²,

Quagliariello³

1972

European Journal of Biochemistry

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1. The relationship between the two elongation syntheses of fatty acids, one present in microsomes and the other in mitochondria, and the system for synthesizing palmitate de novo has been studied in rat liver. The results indicate that microsomes are much more active than mitochondria in elongating and/or clesaturating fatty acids synthesized by cytoplasmic system.2. Fatty acids synthesized by cell sap in the presence of added microsomes are found principally esterified into phospholipids, this indicates that these compounds in the microsomal fraction are actively deacylated and reacylated.3. ATP is required both for chain elongation and for the esterscation of newly synthesized fatty acids. 4.Evidence has been obtained that acyl groups are elongated and/or estcrified into complex lipids as acyl-CoA and not as acyl-ACP.6 . The high activity of palmityl thioesterase in the cell sap with respect to the other subcellular fractions points out the importance of this enzyme in the fatty acid synthesis de novo.

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Degradation of labeled propionic and acetic acids

Cited by 390 publications

References 3 publications

Biosynthesis of Fosfomycin by Streptomyces fradiae

Biosynthesis of Fosfomycin by Streptomyces fradiae

Intermediates of the Pentose Phosphate Pathway as Respiratory Substrates.

Fatty‐Acid Biosynthesis

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