Degradation of phenanthrene, fluorene and fluoranthene by pure bacterial cultures

Weissenfels, Walter D.; Beyer, Michael; Klein, Jürgen

doi:10.1007/bf00903787

Cited by 217 publications

(118 citation statements)

References 21 publications

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“…The method described by T h e m & Fritsche (1995) using the surfactant Sapogenat T-300 as additive was not suitable for selective enrichment of PAH degraders since the detergent could be alternatively utilized as carbon and energy source. The minimal medium described by Kastner et al (1994) was used because it allowed optimal growth of different PAHdegrading reference organisms in comparison to other minimal media tested (Weissenfels et al, 1990;Keuth & Rehm, 1991).…”

Section: Isolation and Taxonomic Affiliation Of The Isolated Pah Degrmentioning

confidence: 99%

Differential detection of key enzymes of polyaromatic-hydrocarbon-degrading bacteria using PCR and gene probes

Moser²,

et al. 1999

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Bacteria with ability to degrade polyaromatic hydrocarbons (PAHs), isolated from wastewater and soil samples, were investigated for their taxonomic, physiological and genetic diversity. Eighteen isolates able to metabolize naphthalene or phenanthrene as sole carbon source were taxonomically affiliated to different subclasses of the Proteobacteria (Sphingomonas spp., Acidovorax spp., Comamonas spp. and Pseudomonas spp.) and to phyla of Gram-positive bacteria with low and high DNA G+C content (Paenibacillus sp. and Rhodococcus spp., respectively). Representatives of the genera Pseudomonas and Sphingomonas formed a remarkably high fraction of these isolates; 9 out of 18 strains belonged to these groups. Tests for enzyme activities showed that the majority of the isolates growing with PAHs as sole sources of carbon and energy had an active catechol2,3-dioxygenase (C230). C230 specific activities were very diverse, ranging from 0-1 to 650 mU (mg protein)-'. Pseudomonas and Sphingomonas strains showed considerably higher activities than the other isolates. All PAH degraders were examined for the presence of an initial PAH dioxygenase and C230, which catalyse key steps of PAH degradation, by PCR amplification of gene fragments and subsequent hybridization. PCR primers and internal oligonucleotide probes were developed for the specific detection of the genes of Pseudomonas and Sphingomonas strains. (1996). The aim of the present investigation was to isolate bacterial PAH degraders from aquatic a n d terrestrial sites, to determine their taxonomic affiliation and to characterize them physiologically a n d genetically with respect to their ability to degrade PAHs. al., 1996) are specific for most genuine pseudornonads, distinct P. putida strains, and the genera Comamonas and Acidovorax, respectively. Keywords METHODSFurther examination of the isolates was done by physiological tests as reported previously (Kampfer et al., 1991). Fatty acid methyl ester (FAME) patterns were used as a further marker for identification. Preparation of FAME extracts, gas chromatographic analysis and numerical identification by the MIDI system (Sherlock 2.11) were performed as described previously (Kampfer et al., 1996).Determination of naphthalene and phenanthrene mineralization potential. The potential of the isolated bacteria for mineralization of PAHs was examined by gas chromatographically quantifying remaining PAH and sirnultaneously monitoring bacterial growth in batch cultures.For each tested strain, five airtight flasks were each filled with 10 rnl basal medium containing either 005 ' / o naphthalene or phenanthrene and were inoculated with approximately 10' cells. Cell number was estimated by measuring the optical density at 600nm, Remaining PAH in the cultures was extracted by vigorously shaking (30 rnin) with 5 rnl n-hexane for gas chromatographic analysis. The gas chromatograph HP 6890 was equipped with a flame-ionization detector and a 25 m capillary column (Ultra 2, cross-linked 5 % phenyl methyl siloxan ; Hewlett Packard) ....

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Section: Isolation and Taxonomic Affiliation Of The Isolated Pah Degrmentioning

confidence: 99%

Differential detection of key enzymes of polyaromatic-hydrocarbon-degrading bacteria using PCR and gene probes

Moser²,

et al. 1999

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“…(Walter et al, 1991), Burkholderia cepacia (Juhasz et al, 1997), Pseudomonas (Juhasz et al, 1996;Johnsen et al, 2005), Stenotrophomonas maltophilia (Boonchan et al, 1998), Mycobacterium sp. (Bouchez et al, 1995), Alcaligenes denitrificans (Weissenfels et al, 1990), and Sphingomonas paucimobilis (Mueller et al, 1990;Ye et al, 1996). Due to the efficiency of S. paucimobilis to biodegrade PAHs of 2-4 condensed benzene rings we have used this bacteria and naphthalene as contaminant, to evaluate the bioremediation in the presence of the new surfactants based on PCL.…”

Section: Introductionmentioning

confidence: 99%

Bioremediation of naphthalene in water by Sphingomonas paucimobilis using new biodegradable surfactants based on poly (ɛ-caprolactone)

Miguel

Peinado

Catalina

et al. 2009

International Biodeterioration & Biodegradation

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a b s t r a c t New amphiphilic block surfactants ABA based on a central segment of polycaprolactone with different molecular composition were evaluated in the bioremediation of naphthalene in water by Sphingomonas paucimobilis and compared with sodium dodecyl sulphate as reference surfactant (SDS). Also the biodegradation of the new surfactants by bacteria, S. paucimobilis and a mixture of bacteria (Pseudomonas aureginosa, Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus megaterium) was studied by indirect impedance technique and carbon dioxide determination. All the bacteria biodegraded in solution and micellar phase the central segment of PCL with mineralization rates in the range of 0.024-0.036 mg of CO 2 per day. S. paucimobilis biodegraded naphthalene in the presence of the new surfactants and GC analysis demonstrated that conversion to products started immediately after inoculum. In all the experiments, except for SDS, at 140 h of incubation time, the remaining naphthalene concentration was about 10% of the initial concentration. In contrast, the production of CO 2 was delayed 4-7 days and values around 75%of naphthalene mineralization degree were achieved in three weeks. The addition of PCL-surfactants, in solution and in micellar phase, not interfered in the naphthalene mineralization. These results have shown promising potential of these biodegradable PCL-surfactants in surfactant-enhanced remediation (SER) technology for removing residual organics from contaminated groundwater and soils.

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“…B. cepacia strain VUN 10003 degraded 1.65 mg of dibenz(a,h)anthracene per litres as a sole carbon oil energy source after 56 days [13]. The result of study which spanned for 56 days of monitoring degradation of anthracene, benzo(a)anthracene and dibenzo(a,h)anthracene in a moderate salt aquatic environment -Bodo creek revealed a fluctuation in quantity of PAH's studied throughout the period monitored with significant reduction on the last day sampled in all the treatment without nutrient amendment [31][32][33]. This trend of degradation in natural brackish water was previously reported during degradation of Phenanthrene, …”

Section: Discussionmentioning

confidence: 99%

Aerobic Degradation of Anthracene, Benzo(a)Anthracene and Dibenzo(a,h) Anthracene by Indigenous Strains of Aerobic Heterotrophic Bacteria and Cyanobacteria Isolates From Crude Oil Contaminated Bodo Creek

Tersagh¹,

Em²,

Bo³

2017

Oil Gas Res

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Biodegradation of anthracene, benzo(a)anthracene and dibenzo(a,h)anthracene by indigenous strains of aerobic heterotrophic bacteria and cyanobacteria isolated from Bodo creek -a moderate salt aquatic environment polluted with crude oil was monitored for 56 days using GC-MS. Anthracene was mineralized to 0.1, 0.001 and 0.08 mg/l by aerobic heterotrophic bacteria cyanobacteria and consortium of aerobic heterotrophic bacteria and cyanobacteria respectively. Benzo(a)anthracene was reduced to 0.03, 0.01 and 0.02 mg/l in the treatments of aerobic heterotrophic bacteria cyanobacteria and consortium of aerobic heterotrophic bacteria and cyanobacteria respectively where as dibenzo(a,h)anthracene was reduced to 0 mg/l in all the treatment options on the 56th day monitored. Fluctuations in the quantity of PAHs monitored and was observed at some weeks since it did not follow a straight and consistent reduction pattern but did not vary significantly with time when compared with the control at p>0.05 since the monitored PAHs were degraded in both the treatment options and the control.

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Degradation of phenanthrene, fluorene and fluoranthene by pure bacterial cultures

Cited by 217 publications

References 21 publications

Differential detection of key enzymes of polyaromatic-hydrocarbon-degrading bacteria using PCR and gene probes

Differential detection of key enzymes of polyaromatic-hydrocarbon-degrading bacteria using PCR and gene probes

Bioremediation of naphthalene in water by Sphingomonas paucimobilis using new biodegradable surfactants based on poly (ɛ-caprolactone)

Aerobic Degradation of Anthracene, Benzo(a)Anthracene and Dibenzo(a,h) Anthracene by Indigenous Strains of Aerobic Heterotrophic Bacteria and Cyanobacteria Isolates From Crude Oil Contaminated Bodo Creek

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