This study was carried out to determine the capability of isolated cyanobacteria species from crude oil polluted Bodo creek. Isolation of pure cultures of cyanobacteria was done and the isolates obtained were identified molecularly on the basis of 16S rRNA gene sequence analysis. The 16S rRNA sequencing for identification of the isolates generated sequences ranging from $600 bp and a 250 bp size PCR amplified fragment. The nucleotide sequence of the 16S rRNA gene was compared with published 16S rRNA sequences using BLAST search at the data base of National Center for Biotechnology Information (NCBI). Biodegradation of petroleum hydrocarbon by the isolates was monitored using GC-FID (Agilent 6890 model) for 49 days. The initial quantity of TPH was 29882 and 753.7 mg LG 1 on day 0 and day 49, respectively. Loss of TPH was statistically significant using one way analysis of variance (ANOVA) p<0.05 with time.

Marine sediments collected from Onne Port, Rivers State Nigeria were manually polluted with tributyltin chloride (TBTCl) to evaluate the abilities of bacterial isolates to degrade the pollutant. Bacterial isolates were screened with at varying concentrations of 3 mM, 5 mM, 7 mM, and 10 mM. Bacterial strains with outstanding capabilities of utilizing TBTCl were biochemically and molecularly characterized. The total bacterial counts varied from 42 × 10 2 to 64.4 × 10 2 cfu/g when plated on MSA only and the viable counts on MSA to 3 mM TBTCl and MSA + 5 mM TBTCl ranged from 22 × 10 1 to 38.5 × 10 2 cfu/g and 18 × 10 1 from 21.9 × 10 1 cfu/g respectively; however, the total viable count in MSA + 10 mM TBTCl ranged from 1.1 × 10 1-3.8 × 10 1 cfu/g. Statistically, total bacterial count varied significantly across the sample sites p < 0.05 (one-way ANOVA). The 2 isolates that showed distinctive growth in the presence of high concentration of TBTCl (10 mM) were molecularly identified as Pseudomonas aeruginosa, and Pseudomonas fluorescens. The 16 SrRNA sequence phylogenetic analysis using BLAST program showed that the isolates were proteobacteria. Since there are scanty works on the mechanisms of the degradation of organotin, it is then important that isolates that can tolerate organotin to a large extent can be used in the cleanup of organotincontaminated site. Pseudomonas aeuroginosa and Pseudomonas fluorescens are candidates for the bioremediation of the contaminated site.

The study of the effect of water guard on microorganisms isolated from domestic water collected and stored for use in various homes in Makurdi metropolis was carried out. A total of 80 samples of water were randomly collected from locations in Makurdi which include; high-level, North bank, Wurukum and Wadata and analyzed for bacteria contamination. Microorganisms isolated from the water before treatment include: Escherichia coli (31.92%), Klebsiella spp (11.39%), Diphtheroid spp (13.94%), Staphylococcus aureus (17.89%), Salmonella typhi (9.42%), Enterobacter aerogenes (8.00%) and Streptococcus faecalis (7.44%). The microorganisms isolated after treatment of water with water guard include: Escherichia coli (49.27%), Klebsiella spp (16.79%), Diphtheroid spp (6.57%), Staphylococcus aureus (27.37%). The most prevalent organism in water after treatment was Escherichia coli due to its presence in almost all the locations after treatment. The result shows significant differences in the occurrence of microorganisms present in untreated and treated water samples at p<0.05. Some of the bacteria isolated before treatment of water with water guard were not seen after treatment. Monitoring of the effect of exposure time of the microbes to water guard is advocated.

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Surface water sources in the oil producing Niger Delta region of Nigeria are highly susceptible to pollution by petroleum hydrocarbons and so it is important to understand the microbial diversity of such ecosystems. Water and sediment samples were collected between April-August, 2013 from Bodo creeks and taken to Environmental Microbiology laboratory of University of Portharcourt for analysis. A total of thirty aerobic heterotrophic bacterial strains isolated ranged from 3.0 -7.0 × 10 4 cfu for surface water and 1.6 -5.6 × 10 4 cfu for sediment samples of Bodo creek using serial dilution and spread plate technique. Pure cultures of bacteria were obtained on the basis of their morphological characteristics and subjected to biochemical tests and further classified on the basis of 16S rRNA gene sequence analysis. The DNA was isolated from size fractionated samples and the diversity of bacteria in each fraction was studied using PCR amplification of partial 16S rRNA. The sequences were submitted to NCBIGen bank for identification and assigning of accession numbers. The isolated aerobic heterotrophic bacteria belong to the families of Enterobacteriaceae, Bacilliceae, Alcaligenaceae, Pseudomonadaceae, Flavobactericeae and Planococcaceae.