Molecular Characterization of Aerobic Heterohophic Bacteria Isolated from Petroleum Hydrocarbon Polluted Brackish Waters of Bodo Creeks, Rivers State Nigeria

Abstract

Surface water sources in the oil producing Niger Delta region of Nigeria are highly susceptible to pollution by petroleum hydrocarbons and so it is important to understand the microbial diversity of such ecosystems. Water and sediment samples were collected between April-August, 2013 from Bodo creeks and taken to Environmental Microbiology laboratory of University of Portharcourt for analysis. A total of thirty aerobic heterotrophic bacterial strains isolated ranged from 3.0 -7.0 × 10 4 cfu for surface water and 1.6 -5.6 × 10 4 cfu for sediment samples of Bodo creek using serial dilution and spread plate technique. Pure cultures of bacteria were obtained on the basis of their morphological characteristics and subjected to biochemical tests and further classified on the basis of 16S rRNA gene sequence analysis. The DNA was isolated from size fractionated samples and the diversity of bacteria in each fraction was studied using PCR amplification of partial 16S rRNA. The sequences were submitted to NCBIGen bank for identification and assigning of accession numbers. The isolated aerobic heterotrophic bacteria belong to the families of Enterobacteriaceae, Bacilliceae, Alcaligenaceae, Pseudomonadaceae, Flavobactericeae and Planococcaceae.

Effect of Bitter Leaves Extract on Bacteria Isolated from Soils of Some Mechanic and non-Mechanic Sites in Makurdi Metropolis

“…The filter supplied the microorganisms with hydrocarbon by vapour phase transfer to the inocula. The plates were placed in the incubator (Gallenkamp Duostat Incubator size 2 England) at 37 0 C for 2 days after which colonies were counted to determine titre (viable counts per unit) of soil samples (Ichor et al, 2014). This was repeated to all 40 samples collected.…”

Enumeration of Total Heterotrophic and Petroleum-degrading Bacteria Counts in Water and Sediments from Diobu Creek, Port Harcourt, Nigeria

“…The results of similar works showed that THBCs and PDBCs were more in wet season compared to dry season and the bacterium with the highest prevalence from oil-polluted water and sediments was Bacillus sp. [1]. In a comparable work in River Nun at Amasoma, Bayelsa State, Nigeria, THBCs 1.5 to 8.67 x 10 5 CFU/g were obtained in sediments [21].…”

“…The ubiquity of bacteria confers on them the ability to inhabit any habitat on planet earth surface, having greater biomass than any other group of organisms. This is achieved by a large surface area to volume ratio, metabolically versatile and obtaining energy by oxidising carbon, parasitism, chemoautotrophy and photoautotrophy [1]. Bacteria play a major role in the biochemical cycling of nitrogen, carbon, sulphur, and oxygen in aquatic environments [2,3].…”

“…The presence of these hydrocarbons in an environment was known to stimulate the presence of hydrocarbons degrading bacteria in the affected environment. The total heterotrophic bacterial and petroleum degrading bacteria counts obtained from oil polluted Bodo Creek in Rivers State, Nigeria; Gokana, Rivers State and Ennore Creek, India, [1,12,13]…”