Degradation of phenol by polymer entrapped microorganisms

Bettmann, Heinz; Rehm, H. J.

doi:10.1007/bf00270587

Cited by 223 publications

(83 citation statements)

References 10 publications

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“…The microorganism was maintained on nutrient agar slant and stored at 4 o C± 1 o C for further use. The mineral salt medium used was modified from the one suggested by Bettman and Rehm (1984). The medium had the following composition per litre : 700 mL deionized water, 100 mL buffer solution A, 100 mL solution B, 50 mL solution C and 50 mL solution D. Compositions of each solution were as follows : Buffer solution A composition K 2 HPO 4 1.0g , KH 2 PO 4 0.5g , (NH 4 ) 2 SO 4 0.5g , deionized water 100ml.…”

Section: Methodsmentioning

confidence: 99%

Kinetics of batch microbial degradation of phenols by indigenous Pseudomonas fluorescence

Agarry

Solomon

2008

Int. J. Environ. Sci. Technol.

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ABSTRACT:The potential of various organisms to metabolize organic compounds has been observed to be a potentially effective means in disposing of hazardous and toxic wastes. Phenols and their compounds have long been recognized as one of the most recalcitrant and persistent organic chemicals in the environment. The bioremediation potential of an indigenous Pseudomonas fluorescence was studied in batch culture using synthetic phenol in water in the concentration range of (100 -500) mg/L as a model limiting substrate. The effect of initial phenol concentration on the degradation process was investigated. Phenol was completely degraded at different cultivation times for the different initial phenol concentrations. Increasing the initial phenol concentration from 100 mg/L to 500 mg/L increased the lag phase from 0 to 66 h and correspondingly prolonged the degradation process from 84 h to 354 h. There was decrease in biodegradation rate as initial phenol concentration increased. Fitting data into Monod kinetic model showed the inhibition effect of phenol The kinetic parameters have been estimated up to initial phenol concentration of 500 mg/ L. The r smax decreased and K s increased with higher concentration of phenol. The r smax has been found to be a strong function of initial phenol concentration. The culture followed substrate inhibition kinetics and the specific phenol consumption rates were fitted to Haldane, Yano and Koga, Aiba et al., Teissier and Webb models. Between the five inhibition models, the Haldane model was found to give the best fit. Therefore, the biokinetic constants estimated using these models showed good potential of the Pseudomonas fluorescence and the possibility of using it in bioremediation of phenol waste effluents.

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Section: Methodsmentioning

confidence: 99%

Kinetics of batch microbial degradation of phenols by indigenous Pseudomonas fluorescence

Agarry

Solomon

2008

Int. J. Environ. Sci. Technol.

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“…The mineral salt medium used was modified from the one suggested by Bettman and Rehm (1984). The medium had the following composition per L: 700 mL deionized water, 100 mL buffer solution A, 100 mL trace elements solution B, 50 mL solution C and 50 mL solution D. Compositions of each solution were as follows: Buffer solution A composition K 2 HPO 4 1.0 g , KH 2 PO 4 0.5 g , (NH 4 ) 2 SO 4 0.5 g , deionized water 100 mL.…”

Section: Culture Medium and Inoculum Preparationmentioning

confidence: 99%

“…Studies on microbial means of treating or removing phenols dates back to at least three decades. Most studies have been on the degradation of much higher concentrations ranging from 1000 -2000 mg/L (Bettman and Rehm, 1984;Schroeder et al, 1997;Sokol, 1988). Only few workers have presented works on phenol degradation of concentrations lower than 250 mg/L (Hill and Robinson, 1975;Lakhwala et al, 1992;Mordocco et al, 1999;Oboirien et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Substrate inhibition kinetics of phenol degradation by Pseudomonas fluorescence from steady state and wash-out data

Agarry

Audu

Solomon

2009

Int. J. Environ. Sci. Technol.

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ABSTRACT:The present study investigated the phenol utilization kinetics of a pure culture of an indigenousPseudomonas fluorescence under steady state and non-steady state (washout) conditions. Steady states of a continuous culture with an inhibitory substrate was used to estimate kinetic parameters under substrate limitation (chemo stat operation) Pure cultures of an indigenous Pseudomonas fluorescence were grown in continuous culture on phenol as the sole source of carbon and energy at dilution rates of 0.010 -0.20/h. Using different dilution rates, several steady states were investigated and the specific phenol consumption rates were calculated. In addition, phenol degradation was investigated by increasing the dilution rate above the critical dilution rate (washout cultivation). The results showed that the specific phenol consumption rate increased with increased dilution rate at steady state and phenol degradation by Pseudomonas fluorescence can be described by simple substrate inhibition kinetics under substrate limitation but cannot be described by simple substrate inhibition kinetics under washout cultivation. Fitting of the steady state data from continuous cultivation to various inhibition models resulted in the best fit for Haldane, Yano and Koga (2), Aiba and Teissier kinetic inhibition models. The r smax value of 0.229 mg/mg/h obtained from the inhibition model equations was comparable to the experimentally calculated r smax value of 0.246 mg/mg/h obtained under washout cultivation. Therefore, the biokinetic constants evaluated using these models showed good tolerance and growth of the indigenous organism.

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“…Zache and Rehm (9) also reported 1.0 gel more phenol degradation with immobilized cells as compared to free cells. The reason for this appears to be protection of cells in immobilized form (3). This study reveals efficient degradation of 2,4-D by immobilized cells as compared to free cells.…”

mentioning

confidence: 63%

Degradation of 2,4-dichlorophenoxy-acetic acid (2,4-D) by immobilizedcells of Pseudomonas putida.

Kochar

Kahlon

1995

J. Gen. Appl. Microbiol.

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Degradation of phenol by polymer entrapped microorganisms

Cited by 223 publications

References 10 publications

Kinetics of batch microbial degradation of phenols by indigenous Pseudomonas fluorescence

Kinetics of batch microbial degradation of phenols by indigenous Pseudomonas fluorescence

Substrate inhibition kinetics of phenol degradation by Pseudomonas fluorescence from steady state and wash-out data

Degradation of 2,4-dichlorophenoxy-acetic acid (2,4-D) by immobilizedcells of Pseudomonas putida.

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