Degradation of Polycyclic Aromatic Hydrocarbons by Fungi

Cerniglia, Carl E.; Sutherland, John B.

doi:10.1007/978-3-540-77587-4_151

Cited by 113 publications

(97 citation statements)

References 209 publications

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“…Less fungal diversity noticed in soil BP-5; only two fungal genera were present, and the genus which inhabited this soil mostly is as yet unidentified. Other studies (Cerniglia and Sutherland 2010;Prince 2010) have also found hydrocarbon-degrading fungi similar to the groups reported in this study.…”

Section: Dynamics Of Hydrocarbon-degrading Microbes In the Contaminatsupporting

confidence: 91%

Evaluation of constraints in bioremediation of weathered hydrocarbon-contaminated arid soils through microcosm biopile study

Ramadass

Smith

Thavamani

et al. 2015

Int. J. Environ. Sci. Technol.

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This research investigated the factors influencing bioremediation (biopile) of arid soils contaminated by weathered hydrocarbons. Five soils were thoroughly characterised to determine total petroleum hydrocarbons (TPH), their physicochemical properties and microbial diversity. Identified biopile-limiting factors are to be elevated petroleum hydrocarbon concentrations, high electrical conductivity and the magnitude of the recalcitrant hydrocarbon fraction. To optimise the biopile parameters, microcosm study was conducted which showed significant TPH reduction in three of five soils (BP-1, BP-2 and BP-4) but not in other two (BP-3 and BP-5), where BP-3 had a very high hydrocarbon concentration (123,757 mg kg -1 ) and BP-5 had a high proportion of recalcitrant hydrocarbons ([70 % of C 29 ). Highest TPH removal (68 %) occurred in soil BP-2 and the lowest (5 %) in soil BP-3 over 56 days. Surfactant (Triton) addition, nutrient amendment or the soil dilution did not improve TPH degradation in soils BP-3 and BP-5. Phylogenetic analysis conducted during the remediation process found that hydrocarbon concentration and hydrocarbon fraction exerted the main effect on bacterial abundance, diversity and assemblage composition. At lower concentrations (*1000-4000 mg kg -1 ), bacterial diversity and abundance increased significantly, whilst decreased in higher concentrations. Although high TPH content and detection of TPH degraders, TPH biodegradation is limited in soil (BP-5) due to the presence of less soluble hydrocarbon fraction which indicated low TPH bioavailability (*7 %). Biopile could be applied as a technology to remediate three soils (BP-1, BP-2 and BP-4) but further modification of the biopile treatments required for other two soils BP-3 and BP-5.

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Section: Dynamics Of Hydrocarbon-degrading Microbes In the Contaminatsupporting

confidence: 91%

Evaluation of constraints in bioremediation of weathered hydrocarbon-contaminated arid soils through microcosm biopile study

Ramadass

Smith

Thavamani

et al. 2015

Int. J. Environ. Sci. Technol.

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“…Fariba et al (2012) found increased enzyme activities in fungal colonies grown in oil-polluted media. An apparent relationship also exists between fungi and the degradation of polycyclic aromatic hydrocarbons (PAHs) (Cerniglia and Sutherland 2010). Nevertheless, the interaction between microorganisms and hydrocarbon is variable from region to region, depending upon the quality and quantity of both.…”

Section: Resultsmentioning

confidence: 99%

Efficiency of Indigenous Filamentous Fungi for Biodegradation of Petroleum Hydrocarbons in Medium and Soil: Laboratory Study from Ecuador

Maddela

Scalvenzi

Pérez

et al. 2015

Bull Environ Contam Toxicol

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The competence of two fungal isolates for degrading petroleum hydrocarbons was evaluated. The filamentous fungi were isolated from a crude oil-contaminated soil in northeastern Ecuador, and were 99 %-100 % similar in 18S rDNA sequence to the genus Geomyces. Their efficiencies of degradation were tested in vitro for 30 days, using medium and soil microcosm. Residual hydrocarbons were tracked by gas liquid chromatography with a flame ionization detector. The maximum removal percentages of total petroleum hydrocarbons were 77.3 % and 79.9 % for experiments in the medium and soil microcosm, respectively. The percent germination of cow pea (Vigna unguiculata) seeds was increased from 20 % to 100 % upon bioremediation. Isolates sporulated optimally on minimal salts agar medium at pH 5, 25°C temperature, 1 %-1.5 % substrate (crude oil) and 4-6 g L(-1) N-P-K. These findings suggest that these fungal isolates are potential degraders for bioremediation in crude oil-contaminated areas in Ecuador.

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“…Morphological changes and interference with germination demonstrate the toxic effect that PAHs have on fungi. Although these tests do not allow us to state the nature of the metabolic activity of PAHs degradation, it is known that PAHs oxidation by non-ligninolytic fungi can form genotoxic compounds that combine with DNA, such as certain trans-9,10-dihydrodiols metabolites, which are soluble in water, but bind to DNA, RNA and proteins, causing direct damage to cells and further carcinogenic effects (Cavalieri et al, 2005;Cerniglia & Sutherland, 2010). Thus, the absorption of phenanthrene and pyrene by fungal hyphae may lead to possible interference in the gene expression of the individual concerned.…”

Section: Discussionmentioning

confidence: 99%

“…Among the Ascomycetes, non-ligninolytic fungi has also been mentioned as promising in bioremediation, due to their capacity to use the intracellular enzyme cytochrome P450 monooxygenase to perform the initial oxidation of the PAH aromatic ring. Together with oxygen originating from arene oxide, followed by epoxide hydrolases and phenol, this can be conjugated to form glucuronide, glycosides, xilosídeos and sulfates, with subsequent mineralization (Cerniglia & Sutherland, 2010). Thus, studies on production of extra and intracellular enzymes should be performed with Hypoxylon sp.…”

Section: Discussionmentioning

confidence: 99%

<b>Tolerance to Polycyclic Aromatic Hydrocarbons (PAHs) by filamentous fungi isolated from contaminated sediment in the Amazon region

Souza

Barreto

Mota

et al. 2017

Acta Sci. Biol. Sci.

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ABSTRACT. Tolerance to Polycyclic Hydrocarbons Aromatic (PAHs) is considered an important characteristic when assessing the bioremediation potential of microorganisms. Given this, the objective of this research was to assay filamentous fungi from the Amazon region, isolated from sediments with differents levels of contamination by PAHs, for tolerance to phenanthrene and pyrene. To achieve this, fungal cultures plugs (5 mm), obtained after 7 days growth, were transferred to petri dishes containing 20% Sabouraud dextrose agar medium, after surface innoculation with phenanthrene and pyrene crystals, separately. Radial mycelial growth was evaluated after 10 days at five different concentration levels for each contaminant and control group, all in triplicate for each treatment. Fungal growth and growth inhibition rates were calculated. The average growth of the colonies in each treatment was compared with one-way ANOVA, followed by a Tukey Test (p < 0,05). All fungi showed tolerant to phenanthrene and pyrene. However, Hypoxylon sp. showed the lowest growth inhibition rate and average growth rates significantly different of the other six tested species. Hypoxylon sp. has been shown to be a promising genetic resource for use in new studies of PAHs degradation.Keywords: phenanthrene, pyrene, fungal growth inhibition, Amazon.Tolerância a Hidrocarbonetos Policíclicos Aromáticos (HPAs) por fungos filamentosos isolados de sedimentos contaminados da região Amazônica RESUMO. A tolerância a Hidrocarbonetos Policíclicos Aromática (HPAs) é considerada como uma característica importante na avaliação do potencial de micro-organismos para biorremediação. Diante disso, o objetivo desta pesquisa foi avaliar fungos filamentosos da região amazônica, isolados de sedimentos com diferentes níveis de contaminação por HPAs, quanto à tolerância ao fenantreno e pireno. Para tanto, discos das culturas fúngicas (5 mm), obtidas após 7 dias de crescimento, foram transferidas para placas de Petri contendo meio Agar Sabouraud Dextrose a 20%, após inoculação superficial com cristais de fenantreno e pireno, separadamente. O crescimento micelial radial foi avaliado após 10 dias em cinco concentrações diferentes para cada contaminante e grupo controle, ambos em triplicata para cada tratamento. As taxas de crescimento fúngico e de inibição de crescimento foram calculadas. O crescimento médio das colônias em cada tratamento foi comparado com ANOVA one way, seguido pelo teste de Tukey (p < 0,05). Todos os fungos mostraram tolerância ao fenantreno e ao pireno. No entanto, Hypoxylon sp. apresentou menor taxa de inibição de crescimento e taxas médias de crescimento significativamente diferentes das outras seis espécies testadas. Hypoxylon sp. tem se mostrado um recurso genético promissor para uso em novos estudos sobre degradação de HPAs.Palavras-chaves: fenantreno, pireno, inibição do crescimento fúngico, Amazônia.

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Degradation of Polycyclic Aromatic Hydrocarbons by Fungi

Cited by 113 publications

References 209 publications

Evaluation of constraints in bioremediation of weathered hydrocarbon-contaminated arid soils through microcosm biopile study

Evaluation of constraints in bioremediation of weathered hydrocarbon-contaminated arid soils through microcosm biopile study

Efficiency of Indigenous Filamentous Fungi for Biodegradation of Petroleum Hydrocarbons in Medium and Soil: Laboratory Study from Ecuador

<b>Tolerance to Polycyclic Aromatic Hydrocarbons (PAHs) by filamentous fungi isolated from contaminated sediment in the Amazon region

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