Degradation of polynuclear aromatic hydrocarbons by two strains of Pseudomonas

Nwinyi, Obinna; Ajayi, Oluseyi O.; Amund, Olukayode O.

doi:10.1016/j.bjm.2016.04.026

Cited by 37 publications

(13 citation statements)

References 28 publications

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“…There are very fewer reports of chrysene degradation in microcosms. Nwinyi et al (2016) observed 14 to 15% degradation of chrysene in 2–3 weeks in laboratory media having a final concentration of 64 mg/kg. But degradation was not examined in microcosm or soil.…”

Section: Resultsmentioning

confidence: 96%

Degradation of Chrysene by Enriched Bacterial Consortium

et al. 2018

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Chrysene is a high molecular weight (HMW), polycyclic aromatic hydrocarbon (PAH) known for its recalcitrance and carcinogenic properties and sparsely soluble (0.003 mg/L) in aqueous medium. Due to these refractory properties, bioavailability of chrysene is very low and therefore is persistence in the environment escaping the metabolism by microorganisms. However, few bacterial and fungal strains are reported to degrade chrysene, but with lower efficiency, requiring additional/extraneous carbon sources (co-substrates) for it’s complete mineralization. In this study, development, enrichment and characterization of bacterial consortium ASDC, consisting of Rhodococcus sp., ASDC1; Bacillus sp. ASDC2; and Burkholderia sp. ASDC3 were reported. Chrysene was utilized as a sole source of carbon and energy by the consortium, having maximum degradation rate of 1.5 mg/L/day and maximum growth rate of 0.125/h, under optimized conditions of pH 7.0, 37°C under aeration of 150 rpm on gyrating shaking. Chrysene degradation was unaffected in presence of other PAHs like pyrene, fluoranthene, naphthalene, phenanthrene, benzene, toluene and xylene, individually as well as in mixture. The results revealed that peptone, ammonium nitrate, sodium succinate have enhanced the chrysene degradation rate during first 24 h of experimentation, which was later on inhibited with increase in incubation time. The chrysene degradation was inhibited by mercury even at lower concentration (1 mM). The results also revealed that SDS has enhanced its degradation by 5.2-fold for initial 24 h of growth, but with increasing in the incubation period, it decreases by 1.2-fold on 7th day of experimentation. The HPLC studies suggested that chrysene was degraded through phthalic acid pathway by the consortium ASDC and the stoichiometric measurements indicated the complete mineralization of chrysene. The flask scale results were validated at simulated microcosm models, where enriched consortium ASDC exhibited maximum degradation (96%) in polluted, non-sterile soil sediment, indicating that consortial strains along with indigenous metabolism showed synergistic metabolism for degradation of chrysene. Thus, the above study revealed the useful enrichment of bacterial community for synergistic degradation of PAHs (chrysene) which could be further exploited for in situ remediation of PAH contaminated sites.

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Section: Resultsmentioning

confidence: 96%

Degradation of Chrysene by Enriched Bacterial Consortium

et al. 2018

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“…DNA was screened for eight different beta-lactamase genes, including ESBLs (SHV, TEM and CTX-M genes), Oxacillinase (OXA) and carbapenemases (IMP, VIM, KPC and NDM genes). Beta-lactamase genes were screened for using specific primers (Table 3) as previously described [55][56][57][58]. For polymerase chain reaction (PCR) amplification of beta-lactamase genes in a thermocycler C100 Touch (Bio-RAD, USA), 5 µL aliquot of DNA template was added to a 20 µL master mix.…”

Section: Dna Extraction and Genotypic Detection Of β-Lactamase Genesmentioning

confidence: 99%

First Detection of Carbapenem-Resistant Escherichia fergusonii Strains Harbouring Beta-Lactamase Genes from Clinical Samples

Adesina

Nwinyi

et al. 2019

Pathogens

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Recently discovered extraintestinal Escherichia fergusonii obtained from non-clinical samples has exhibited the potential for acquiring multiple beta-lactamase genes, just like many extraintestinal Escherichia coli strains. Albeit, they are often omitted or classified as E. coli. This study aimed to, therefore, identify carbapenem-resistant extended-spectrum beta-lactamase (ESBL) producing E. fergusonii isolates from clinical samples, determine their evolutionary relatedness using 16S rRNA sequencing analysis and screen for beta-lactamase genes. A total of 135 septic wound samples were obtained from patients on referral at a General Hospital in Lagos, Nigeria. For the phenotypic identification of isolates from culture-positive samples, morphological, and physiological tests were carried out. Identities of the isolates harbouring beta-lactamase genes were assigned to their genus strains using the 16S rRNA sequencing. The Kirby Bauer disc diffusion technique and double-disc synergy test were used to screen isolates for multidrug resistance and ESBL production. Carbapenem-resistant ESBL producing isolates were screened for beta-lactamase genes in a polymerase chain reaction. Three E. fergusonii isolates (CR11, CR35 and CR49) were obtained during this study. E. fergusonii strains were motile, non-lactose and non-sorbitol fermenting but positive for cellobiose and adonitol fermentation. The I6S rRNA assigned the phenotypically identified isolates to E. fergusonii species. All three isolates were multidrug-resistant, carbapenem-resistant and ESBL producers. Isolates CR11 and CR35 harboured cefotaximase (CTX-M) and temoniera (TEM) beta-lactamase genes while CR49 harboured sulfhydryl variable (SHV) beta-lactamase gene. We herein report the detection of multiple beta-lactamase genes in carbapenem-resistant ESBL producing E. fergusonii from clinical samples.

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“…The high incidence of Pseudomonas species in naphthalene enrichments, as well as their PAH biodegradation ability, has been widely reported (Amini et al 2017;Nwinyi et al 2016;Thomas et al 2016;Patowary et al 2015;Wald et al 2015;Ma et al 2012;Obayori et al 2008;Ma et al 2006;Leahy and Colwell 1990;Foght and Westlake 1988). However, this is the first study reporting the naphthalene biodegradation ability of P. silesiensis, P. arsenicoxydans, P. umsongensis, P. laurylsulfatiphila, P. mandelii, and P. laurentiana; so far, no other PAH compound degradation ability of isolates affiliated with these species has been reported either.…”

Section: Discussionmentioning

confidence: 81%

Aerobic and oxygen-limited naphthalene-amended enrichments induced the dominance of Pseudomonas spp. from a groundwater bacterial biofilm

Benedek

Szentgyörgyi

Szabó

et al. 2020

Appl Microbiol Biotechnol

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In this study, we aimed at determining the impact of naphthalene and different oxygen levels on a biofilm bacterial community originated from a petroleum hydrocarbon–contaminated groundwater. By using cultivation-dependent and cultivation-independent approaches, the enrichment, identification, and isolation of aerobic and oxygen-limited naphthalene degraders was possible. Results indicated that, regardless of the oxygenation conditions, Pseudomonas spp. became the most dominant in the naphthalene-amended selective enrichment cultures. Under low-oxygen conditions, P. veronii/P. extremaustralis lineage affiliating bacteria, and under full aerobic conditions P. laurentiana–related isolates were most probably capable of naphthalene biodegradation. A molecular biological tool has been developed for the detection of naphthalene 1,2-dioxygenase-related 2Fe-2S reductase genes of Gram-negative bacteria. The newly developed COnsensus DEgenerate Hybrid Oligonucleotide Primers (CODEHOP-PCR) technique may be used in the monitoring of the natural attenuation capacity of PAH-contaminated sites. A bacterial strain collection with prolific biofilm-producing and effective naphthalene-degrading organisms was established. The obtained strain collection may be applicable in the future for the development of biofilm-based bioremediation systems for the elimination of PAHs from groundwater (e.g., biofilm-based biobarriers).

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Degradation of polynuclear aromatic hydrocarbons by two strains of Pseudomonas

Cited by 37 publications

References 28 publications

Degradation of Chrysene by Enriched Bacterial Consortium

Degradation of Chrysene by Enriched Bacterial Consortium

First Detection of Carbapenem-Resistant Escherichia fergusonii Strains Harbouring Beta-Lactamase Genes from Clinical Samples

Aerobic and oxygen-limited naphthalene-amended enrichments induced the dominance of Pseudomonas spp. from a groundwater bacterial biofilm

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