Degradation of Profenofos and λ-Cyhalothrin Using Endogenous Bacterial Isolates and Detection of the Responsible Genes

Abdullah, Reda R; Abde, Sherif B; Ghani,

doi:10.4172/2155-6199.1000360

Cited by 6 publications

(1 citation statement)

References 4 publications

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“…Opd and mpd genes appear to be indifferent bacterial strains as Opd genes have been found in Agrobacterium radiobacter, Flavobacterium sp, Pseudomonas diminuta, pseudomonas putida, plesiomonas species, Sphingomonas sp (Zhongli et al, 2001;Horne et al, 2002;and Iyer et al, 2013). While, mpd gene was detected and isolated from Plesiomonas sp., Sphingomonas sp., Pseudomonas sp., Stenotrophomonas sp., (Cui et al, 2001;Li et al, 2007;Liu et al, 2005;Yang et al, 2006 andAbdullah et al, 2016). PCR has been put to work for genotyping bacteria quickly and accurately according to degradation genes.…”

Section: Discussionmentioning

confidence: 99%

Biodegradation of Chlorpyrifos by Some Bacterial Strains and Screening Their Degraded Genes

Shoman

Aggag

Yacout

2022

Journal of Agricultural Chemistry and Biotechnology

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Biodegradation is a natural process, where the degradation of a xenobiotic chemical or pesticide by an organism is primarily a strategy for their survival. Pesticides are degraded in the environment principally by the action of microorganisms. Evaluate the ability of three bacterial strains to chlorpyrifos-degrade, determine the chlorpyrifos degrading genes in the isolates, study their biochemical mode of degradation. Three bacterial strains i.e., Pseudomonas fluorescens, Rhizobium leguminosarum, and Bacillus megaterium were detected in Luria-Bertani (LB) medium degrading Chlorpyrifos at day 14. Microbiological, biochemical studies and genetic factors in biodegradation were studied. P. fluorescens had the highest efficiency with 58.90%, then R. leguminosarum 56.72%, and finally B. megaterium with 50.69%. According to the genetic similarity for degradation genes, B. megaterium and P. fluorescens have nearly the same, while R. leguminosarum has another pathway. P. florescence and R. Leguminosarum strains were the highest in the degradation process which analyzed the Chlorpyrifos component to 3,5,6-trichloro-2-pyridinol (TCP) and Chlorpyrifos Oxon that has a little toxic effect on the ecosystem. With the obtained results, it is recommended to apply these strains on soil and plants to reduce the toxicity of chlorpyrifos in the environment.

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Section: Discussionmentioning

confidence: 99%