Degradation of ribosomal precursor and polyadenylic acid-containing ribonucleic acids in Saccharomyces cerevisiae caused by actinomycin D

Interaction of actinomycin D with yeast ribosomal RNA

“…Since the interaction between AM and rRNA depends upon the secondary structure of RNA, one can suggest a non-random binding of AM to 37 S pre-rRNA. The binding of several AM molecules to small parts of the 37 S pre-rRNA could change the structure of the ribonucleoprotein particles and promotes degradation, rather than maturation of pre-rRNA, as observed in [2]. Abnormal processing of rRNA was also found in yeast cells treated with the intercalating agent ethidium bromide [ 111. …”

“…Total RNA was extracted from Saccharomyces cerevisiae VY1160 and extensively deproteinized as in [2]. rRNA was freed of DNA fragments, 'ds' RNA and tRNA by repeated precipitation with 2 M LiCl.…”

“…This resistance is most likely due to poor permeability, since a mutant characterized by increased permeability to different antibiotics [l] is sensitive to AM. Antibiotic at lo-20 pg/ml caused a complete arrest of RNA synthesis, but a posttranscriptional degradation of rRNA precursors (prerRNA) was also observed [2]. An AM-sensitive mutant of Saccharomyces cerevisiae has been isolated [3].…”

Suppression of sorbitol dependence in a strain bearing a mutation in the SRB1/PSA1/VIG9 gene encoding GDP-mannose pyrophosphorylase by PDE2 overexpression suggests a role for the Ras/cAMP signal-transduction pathway in the control of yeast cell-wall biogenesis

“…Work on strains carrying the srb1-1 mutation found a number of cell-wall changes, including alterations in glucan structure and composition (Blagoeva et al, 1991), and mannan content (Maerkisch et al, 1983). Investigations on the mode of action of actinomycin D (Waltschewa et al, 1976) and rifampicin (Venkov et al, 1975) have been facilitated by the hypersensitivity to antibiotics shown by strains carrying the srb1-1 mutation.…”

The use of D‐galactosamine for a pulse‐chase study of ribosomal RNA maturation in rat liver

“…Studies with actinomycin D, camptothecin and other inhibitors interacting with DNA or chromatin showed an inhibition of pre-rRNA processing and led to the suggestion that continuous transcription of rRNA genes is directly coupled with the formation and nucleo-cytoplasmic transfer of ribosomes [ 1,2] . However, the action of actinomycin D on pre-rRNA processing may be independent of its effect on transcription [3] and similar side effects are possible with other drugs interacting with DNA. Therefore, studies with different inhibitors of RNA synthesis, which would not alter chromatin structure, are necessary in order to elucidate the mechanisms controlling prerRNA processing and ribosome formation.…”