Degradation of styrene and ethylbenzene byPseudomonasspecies Y2

Abstract

Summary Pseudomonas species Y2 is able to grow on styrene, ethylbenzene, 2‐phenylethanol, 1‐phenylethanol, acetophenone, phenylacetate, and mandelate as a sole source of carbon and energy. Ethylbenzene is transformed by Y2 cells to yield 1‐phenylethanol, 2‐phenylethanol, phenylacetate, and four novel products: acetophenone, salicylate, 2‐hydroxyphenylacetate, and mandelate. Salicylate, 2‐hydroxyphenylacetate, 1‐ and 2‐phenylethanol are produced from styrene. The formation of 1‐phenylethanol and salicylate from styrene has not been previously described. Our growth experiments have shown that styrene and ethylbenzene are catabolized via 2‐phenylethanol and phenylacetate. It is the first report of the ethylbenzene pathway. Ethylbenzene is also catabolized via 1‐phenylethanol. The induction of catechol 2,3‐dioxygenase by growing the organism on ethylbenzene and 1‐pheny lethanol indicates that these compounds could be degraded via the corresponding catechols and then via extradiol cleavage of the aromatic ring.

Isolation and Identification of Styrene-degradingCorynebacteriumStrains, and Their Styrene Metabolism

“…On the other hand, the Gram-negative rod type bacteria GP-I strain was identified as a Pseudomonas sp. It has already been reported by Shirae and Hisatsuka,6) Baggi et al,B) Utkin et al, 9) and Rustemov et al,IO) as a styrene-degrading strain, so we focused on the Corynebacterium strains. As far as we know, this is the first report on a styrene-degrading Corynebacterium sp.…”

Metabolic pathways responsible for consumption of aromatic hydrocarbons by microbial associations: Molecular-genetic characterization

“…Cultures degrading particular xenobiotics were introduced into pilot flow minireactors for the elimination of VOCs from air [1][2][3]. The overall VOC load on each minireactor was ~300-350 mg/m 3 .…”

Application of molecular systematics to study of bacterial cultures consuming volatile organic compounds

“…Cultures degrading particular xenobiotics were placed into flow pilot minireactors for air purification from VOCs [1][2][3][4]. The overall VOC load on each minireactor was 300-350 mg/m 3 .…”

“…At present, owing to the rapid development of industrial biotechnology, microbiological methods are in increasing use for purification of industrial waste air containing volatile organic compounds (VOCs) [1][2][3][4].…”