Degradation of sulfated polysaccharide extracted from algal Laminaria japonica and its modulation on calcium oxalate crystallization

Ouyang, Jing; Wang, Miao; Lü, Peng; Tan, Jin Han

doi:10.1016/j.msec.2010.05.002

Cited by 19 publications

(13 citation statements)

References 25 publications

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“…Currently, the main prescription drugs for treatment of urinary calculi are citrate, magnesium preparations, orthophosphate, allopurinol, and thiazide diuretics. However, the action mechanism of these drugs remains unclear, and their curative effects can be marginal [ 13 ]. Thus, scholars must develop new highly efficient, nontoxic, and inexpensive anti-stone drugs for scientific and practical applications [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Structural Characterization and Repair Mechanism of Gracilaria lemaneiformis Sulfated Polysaccharides of Different Molecular Weights on Damaged Renal Epithelial Cells

Guo

Sun

et al. 2018

Oxidative Medicine and Cellular Longevity

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Natural Gracilaria lemaneiformis sulfated polysaccharide (GLP0, molecular weight = 622 kDa) was degraded by H2O2 to obtain seven degraded fragments, namely, GLP1, GLP2, GLP3, GLP4, GLP5, GLP6, and GLP7, with molecular weights of 106, 49.6, 10.5, 6.14, 5.06, 3.71, and 2.42 kDa, respectively. FT-IR and NMR results indicated that H2O2 degradation does not change the structure of GLP polysaccharides, whereas the content of the characteristic −OSO3H group (13.46% ± 0.10%) slightly increased than that of the natural polysaccharide (13.07%) after degradation. The repair effects of the polysaccharide fractions on oxalate-induced damaged human kidney proximal tubular epithelial cells (HK-2) were compared. When 60 μg/mL of each polysaccharide was used to repair the damaged HK-2 cells, cell viability increased and the cell morphology was restored, as determined by HE staining. The amount of lactate dehydrogenase released decreased from 16.64% in the injured group to 7.55%–13.87% in the repair groups. The SOD activity increased, and the amount of MDA released decreased. Moreover, the mitochondrial membrane potential evidently increased. All polysaccharide fractions inhibited S phase arrest through the decreased percentage of cells in the S phase and the increased percentage of cells in the G2/M phase. These results reveal that all GLP fractions exhibited repair effect on oxalate-induced damaged HK-2 cells. The repair ability is closely correlated with the molecular weight of the fractions. GLP2 with molecular weight of about 49.6 kDa exhibited the strongest repair effect, and GLP with higher or lower molecular weight than 49.6 kDa showed decreased repair ability. Our results can provide references for inhibiting the formation of kidney stones and developing original anti-stone polysaccharide drugs.

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Section: Introductionmentioning

confidence: 99%

Structural Characterization and Repair Mechanism of Gracilaria lemaneiformis Sulfated Polysaccharides of Different Molecular Weights on Damaged Renal Epithelial Cells

Guo

Sun

et al. 2018

Oxidative Medicine and Cellular Longevity

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“…Not every anionic molecule can interact with the oxalate crystals and interfering with their formation [29]. However, when they interact with the crystals, changes in the morphology [14,15] and size of the crystals can occur [16,17,29]. For example, Wesson and collaborators [29] demonstrated that anionic proteins tend to increase the number of crystals and decrease their size since these negatively charged proteins interact more with the rich calcium faces of both COM and COD crystals, blocking their growth.…”

Section: Resultsmentioning

confidence: 99%

“…The studies are done in vitro as well as with cells under culture conditions and in vivo. The data are well reviewed in a recently published paper [13], which verifies these polymers can inhibit the formation of CaOx renal stone formation in different ways: they inhibit crystallization (both in the nucleation phase [14,15,16] and in growth phase [16,17]); they inhibit the aggregation phase [14,15,16,17,18]; they inhibit the occurrence of COM crystals and the transformation of COM to COD [14,16,17,18]; and they inhibit renal tubular cell injury in vivo [19,20,21].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata

et al. 2019

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Urolithiasis affects approximately 10% of the world population and is strongly associated with calcium oxalate (CaOx) crystals. Currently, there is no efficient compound that can be used to prevent this disease. However, seaweeds’ sulfated polysaccharides (SPs) can change the CaOx crystals surface’s charge and thus modify the crystallization dynamics, due to the interaction of the negative charges of these polymers with the crystal surface during their synthesis. We observed that the SPs of Caulerpa cupressoides modified the morphology, size and surface charge of CaOx crystals. Thus, these crystals became similar to those found in healthy persons. In the presence of SPs, dihydrate CaOx crystals showed rounded or dumbbell morphology. Infrared analysis, fluorescence microscopy, flow cytometry (FITC-conjugated SPs) and atomic composition analysis (EDS) allowed us to propose the mode of action between the Caulerpa’s SPs and the CaOx crystals. This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and CaOx crystals, which are the main cause of kidney stones.

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“…C 6 S is the major component of GAGs responsible for their inhibitory effect. The inhibitory mechanisms of C 6 S include the following [38].

C 6 S is a linear polysaccharide polyanion.

…”

Section: Resultsmentioning

confidence: 99%

Stabilization of Submicron Calcium Oxalate Suspension by Chondroitin Sulfate C May Be an Efficient Protection from Stone Formation

Xue

Ouyang

2013

Bioinorganic Chemistry and Applications

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The influences of chondroitin sulfate C (C6S) on size, aggregation, sedimentation, and Zeta potential of sub-micron calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) crystallites with mean sizes of about 330 nm were investigated using an X-ray diffractometer, nanoparticle size Zeta potential analyzer, ultraviolet spectrophotometer, and scanning electron microscope, after which the results were compared with those of micron-grade crystals. C6S inhibited the conversion of COD to COM and the aggregation of COM and COD crystallitesis; it also decreased their sedimentation rate, thus increasing their stability in aqueous solution. The smaller the size of the COD crystallites, the easier they can be converted to COM. The stability of sub-micron COD was worse than that of micron-grade crystals. C6S can inhibit the formation of calcium oxalate stones.

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Degradation of sulfated polysaccharide extracted from algal Laminaria japonica and its modulation on calcium oxalate crystallization

Cited by 19 publications

References 25 publications

Structural Characterization and Repair Mechanism of Gracilaria lemaneiformis Sulfated Polysaccharides of Different Molecular Weights on Damaged Renal Epithelial Cells

Structural Characterization and Repair Mechanism of Gracilaria lemaneiformis Sulfated Polysaccharides of Different Molecular Weights on Damaged Renal Epithelial Cells

In Vitro Studies Reveal Antiurolithic Effect of Antioxidant Sulfated Polysaccharides from the Green Seaweed Caulerpa cupressoides var flabellata

Stabilization of Submicron Calcium Oxalate Suspension by Chondroitin Sulfate C May Be an Efficient Protection from Stone Formation

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