Degradation regulated bioactive hydrogel as the bioink with desirable moldability for microfluidic biofabrication

Liu, Xiaolu; Zuo, Yicong; Sun, Jing; Guo, Zhenzhen; Fan, Hongsong; Zhang, Xingdong

doi:10.1016/j.carbpol.2017.09.014

Cited by 24 publications

(28 citation statements)

References 40 publications

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“…On the other hand, an initial weight loss of scaffolds can be further elucidated also with the corresponding swelling profile (Figure 3), described in the previous section. The swelling equilibrium that was reached after 30 h coincides with the lowest measured weight (Figure 4), implying a possible impairment of the ALG/CMC network [24] that loosened the "internal stress" of the bioink formulation, and consequently caused some deformability [52]. In turn, degradation of scaffolds could have enlarged internal voids, allowing further water uptake (and cell growth media, affecting even nutrient diffusion/distribution rates), which can in turn promote cell migration (more space for cells to move between these voids) and potentially also proliferation.…”

Section: In Vitro Degradation Of the 3d Bioprinted Scaffoldsmentioning

confidence: 84%

“…The obtained results from the Live/Dead staining suggest a favorable degradation rate of the hSF-laden scaffolds for further development of skin tissue models/substitutes. Namely, the observed formation of the cell aggregates with the prolonged culturing time, suggests that a continuous degradation of the ALG/CMC/NFC scaffolds, which is in turn beneficial for cell adhesion, propagation and aggregation, is present [52]. The formation of cell aggregates further indicates an increased cell proliferation and the establishment of intercellular communication, which plays a vital role in the inhibition of apoptosis (indirectly observed among others through the absence of red-dyed dead cells after 29 days of culturing) [60].…”

Section: Live/dead Assay To Evaluate the Viability Of The Cell-ladenmentioning

confidence: 99%

“…Stiffer networks (in scaffolds), which are less prone to deform, may hinder cellular function. Since cells need sufficient space to proliferate, the reduction of the scaffolds lattice rigidity is vital for their spreading and proliferation [52,53].…”

Section: In Vitro Degradation Of the 3d Bioprinted Scaffoldsmentioning

confidence: 99%

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Polysaccharide-Based Bioink Formulation for 3D Bioprinting of an In Vitro Model of the Human Dermis

et al. 2020

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Limitations in wound management have prompted scientists to introduce bioprinting techniques for creating constructs that can address clinical problems. The bioprinting approach is renowned for its ability to spatially control the three-dimensional (3D) placement of cells, molecules, and biomaterials. These features provide new possibilities to enhance homology to native skin and improve functional outcomes. However, for the clinical value, the development of hydrogel bioink with refined printability and bioactive properties is needed. In this study, we combined the outstanding viscoelastic behavior of nanofibrillated cellulose (NFC) with the fast cross-linking ability of alginate (ALG), carboxymethyl cellulose (CMC), and encapsulated human-derived skin fibroblasts (hSF) to create a bioink for the 3D bioprinting of a dermis layer. The shear thinning behavior of hSF-laden bioink enables construction of 3D scaffolds with high cell density and homogeneous cell distribution. The obtained results demonstrated that hSF-laden bioink supports cellular activity of hSF (up to 29 days) while offering proper printability in a biologically relevant 3D environment, making it a promising tool for skin tissue engineering and drug testing applications.

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Section: In Vitro Degradation Of the 3d Bioprinted Scaffoldsmentioning

confidence: 84%

Section: Live/dead Assay To Evaluate the Viability Of The Cell-ladenmentioning

confidence: 99%

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Polysaccharide-Based Bioink Formulation for 3D Bioprinting of an In Vitro Model of the Human Dermis

et al. 2020

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“…Being able to encapsulate several molecules, alginate can therefore be used to deliver biomolecules and other factors [149]. In addition, alginate-based bioinks can form hollow structures, making them useful in making microfluidic chips [150][151][152]. Alginate when used together with cartilage progenitor cells formed tubular structures able to support cartilage progenitor cell growth [153].…”

Section: Other Bioinks Utilized In 3d Bioprintingmentioning

confidence: 99%

Trends in Decellularized Extracellular Matrix Bioinks for 3D Printing: Inspiring a new Kind of Medicine

Dzobo¹,

Motaung²,

Adesida³

2019

Preprint

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The promise of regenerative medicine and tissue engineering is founded on the ability to regenerate diseased or damaged tissues and organs into functional tissues and organs or the creation of new tissues and organs altogether. In theory, all damaged and diseased tissues and organs can be regenerated or created using different configurations and combinations of extracellular matrix, cells and inductive biomolecules. Currently, regenerative medicine and tissue engineering can allow the improvement of patients’ quality of life through availing novel treatment options. Tissues and organs have a specific ECM, with specific proteins and factors released by cells residing within the local microenvironment. The coupling of regenerative medicine and tissue engineering field with 3D printing is revolutionizing the treatment of patients in a huge way. 3D bioprinting allows the proper placement of cells and ECMs, allowing the recapitulation of native microenvironments of tissues and organs. 3D bioprinting utilizes different bioinks made up of different formulations of ECM/biomaterials, biomolecules and even cells. The choice of the bioink used during 3D bioprinting is very important as properties such as printability, compatibility and physical strength influence the final construct printed. The extracellular matrix (ECM) provides both physical and mechanical microenvironment needed by cells to survive and proliferate. Decellularized ECM bioink contains biochemical cues from the original native ECM and also the right proportions of ECM proteins. Different techniques and characterization methods are used to derive bioinks from several tissues and organs and to evaluate their quality. This review discusses the uses of decellularized ECM bioinks and argues that they represent the most biomimetic bioinks available. In addition, we briefly discuss some polymer-based bioinks utilized in 3D bioprinting.

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“…Up to date, many tissue types, such as bone, vasculature, neural tissue and cardiac muscle, have been created starting from autologous and patient specific primary or stem cells [141][142][143] . The basic unit of the entire printing process is the socalled "bioink" that has to be biocompatible and stable from a mechanical and structural point of view [144,145] . Generally, the printable material is composed by living cells, such as embryonic, adult, or induced pluripotent stem cells, proteins and other active biological molecules loaded into a matrix, mimicking the ECM.…”

Section: Bioprintingmentioning

confidence: 99%

CPCs and ECM: A Good Mix for Cardiac Regeneration

2018

Med One

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Despite several improvements in term of diagnosis and prevention, ischemic heart disease still represents one of the principal worldwide causes of death. Cardiac progenitor cells (CPCs) based therapy is considered a valid alternative to heart transplant, but several issues concerning the transplanted cells viability, retention and therapeutic effect need to be solved. Tissue engineering, mixing synthetic or natural polymers with injected cells, could represent the way through which overcomes shortages and set up an effective cardiac regenerative therapy. Nowadays, it is well known that cardiac extracellular matrix (ECM) provides structural and functional integrity, affects cardiac function, development and physiologic repair. In this optic, ECM and ECM-like materials represent functional and biocompatible tools with a great potential to serve as natural or nature-mimicking scaffolds in the field of regenerative medicine. The aim of the present work is to provide an overview on the state of the art and recent advantages on CPCs and scaffold-based therapy for heart regeneration.

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Degradation regulated bioactive hydrogel as the bioink with desirable moldability for microfluidic biofabrication

Cited by 24 publications

References 40 publications

Polysaccharide-Based Bioink Formulation for 3D Bioprinting of an In Vitro Model of the Human Dermis

Polysaccharide-Based Bioink Formulation for 3D Bioprinting of an In Vitro Model of the Human Dermis

Trends in Decellularized Extracellular Matrix Bioinks for 3D Printing: Inspiring a new Kind of Medicine

CPCs and ECM: A Good Mix for Cardiac Regeneration

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