Degradative Enzyme Production by Salt-Marsh Fungi

Gessner, Robert

doi:10.1515/botm.1980.23.2.133

Cited by 60 publications

(31 citation statements)

References 14 publications

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“…Highest lipolytic activity (C9 mm) was observed for Lasiodiplodia theobromae followed by Aspergillus niger and Colletotrichum gloeosporioides, and lesser so for Chalaropsis thielavioides, and Phoma glomerata (Table 4). Similar observations have been reported for lipolytic activity of higher marine fungi from salt marshes (Gessner 1979).…”

Section: Resultssupporting

confidence: 89%

Diversity of plant oil seed-associated fungi isolated from seven oil-bearing seeds and their potential for the production of lipolytic enzymes

Venkatesagowda

Ponugupaty

Barbosa

et al. 2011

World J Microbiol Biotechnol

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Commercial oil-yielding seeds (castor, coconut, neem, peanut, pongamia, rubber and sesame) were collected from different places in the state of Tamil Nadu (India) from which 1279 endophytic fungi were isolated. The oil-bearing seeds exhibited rich fungal diversity. High Shannon-Index H' was observed with pongamia seeds (2.847) while a low Index occurred for coconut kernel-associated mycoflora (1.018). Maximum Colonization Frequency (%) was observed for Lasiodiplodia theobromae (176). Dominance Index (expressed in terms of the Simpson's Index D) was high (0.581) for coconut kernel-associated fungi, and low for pongamia seed-borne fungi. Species Richness (Chao) of the fungal isolates was high (47.09) in the case of neem seeds, and low (16.6) for peanut seeds. All 1279 fungal isolates were screened for lipolytic activity employing a zymogram method using Tween-20 in agar. Forty isolates showed strong lipolytic activity, and were morphologically identified as belonging to 19 taxa (Alternaria, Aspergillus, Chalaropsis, Cladosporium, Colletotrichum, Curvularia, Drechslera, Fusarium, Lasiodiplodia, Mucor, Penicillium, Pestalotiopsis, Phoma, Phomopsis, Phyllosticta, Rhizopus, Sclerotinia, Stachybotrys and Trichoderma). These isolates also exhibited amylolytic, proteolytic and cellulolytic activities. Five fungal isolates (Aspergillus niger, Chalaropsis thielavioides, Colletotrichum gloeosporioides, Lasiodiplodia theobromae and Phoma glomerata) exhibited highest lipase activities, and the best producer was Lasiodiplodia theobromae (108 U/mL), which was characterized by genomic sequence analysis of the ITS region of 18S rDNA.

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Section: Resultssupporting

confidence: 89%

Diversity of plant oil seed-associated fungi isolated from seven oil-bearing seeds and their potential for the production of lipolytic enzymes

Venkatesagowda

Ponugupaty

Barbosa

et al. 2011

World J Microbiol Biotechnol

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“…However, greater activity against soluble cellulose (CMC) than the crystalline cellulose (filter paper) was found. This result is in agreement with similar study on salt-marsh fungi carried out by Gessner (1980). However, Lemonniera aquatic, Lunulospora curvula, Beverwykella pulmonaria and Helicodendron intestinale showed good ability to produce Cx cellulase enzyme.…”

Section: Resultssupporting

confidence: 92%

“…The oxidation of the tannic acid was considered to be a positive test for polyphenol oxidase activity, which is involved in the degradation of lignin (Davidson et al, 1938;Gessner, 1980;and Trigiano & Fergus, 1979). Of the 19 isolates tested, Beverwykella pulmonaria and Helicodendron intestinale showed the highest activity.…”

Section: Resultsmentioning

confidence: 99%

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Extracellular enzymatic activity of aquatic and aero-aquatic conidial fungi

Abdullah

Taj-Aldeen

1989

Hydrobiologia

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Nineteen species of aquatic and areo-aquatic conidial fungi were tested for their ability to produce extracellular enzymes which degrade cellulose, starch, lipids, proteins and tannic acid. The cellulolytic activity was determined by using both solid and liquid media. The activity of other enzymes was examined using solid media. Two-thirds of the species were able to hydrolyze soluble cellulose (CMC) incorporated in solid and liquid media with varying degrees of activity. Extracellular culture filtrates ofdegetita candida, Helicodendron giganteum and H. tubulosum contained a Cl-Cx enzyme complex that could degrade both soluble cellulose (CMC) and crystalline cellulose (filter paper). Lipase activity was demonstrated by 11 species. Fourteen of the species showed activity for amylase and protease, but only 11 of the 16 were capable of degrading tarmic acid.

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“…Enzyme activities were measured qualitatively by means of the halo generated by the degradative activity of the produced enzymes. (Pointing, 1999), pectinases with Ruthenium Red (Hankin & Anagnostakis, 1977), and amylases with I 2 -KI (Gessner, 1980). Lipases were detected by the presence of a precipitate around the fungal colonies caused by the formation of lauric acid calcium salt crystals (Abdel-Raheem & Shearer, 2002).…”

Section: Identificationmentioning

confidence: 99%