Diversity of plant oil seed-associated fungi isolated from seven oil-bearing seeds and their potential for the production of lipolytic enzymes

Venkatesagowda, Balaji; Ponugupaty, Ebenezer; Barbosa, Aneli M.; Dekker, Robert F. H.

doi:10.1007/s11274-011-0793-4

Cited by 52 publications

(31 citation statements)

References 35 publications

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“…Aa_27 endophyte had high ability to produce most of the tested extracellular enzymes, unlike to Sh_26 endophyte which only able to degrade cellulose and pectin. Cellulolytic and pectinolytic activities were prominent in all fungal endophytes where xylanolytic activity was found in Pc_25, and Aa_27 endophytes; similarly, Venkatesagowda et al (2012) and Sunitha et al (2013) recorded high activity of cellulase and pectinase enzymes in endophytic fungi isolated from oil-seeds and medicinal plants. Production of hydrolytic extracellular enzymes by fungal endophytes in particular cellulase and pectinase acts as a bioactivity to obtain nutrients from hosts and bio-resistance action against microbial pathogenic infection.…”

Section: Discussionmentioning

confidence: 63%

Biotechnological applications of fungal endophytes associated with medicinal plant Asclepias sinaica (Bioss.)

Fouda

Hassan

Eid

et al. 2015

Annals of Agricultural Sciences

208

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Fungal endophytes associated with medicinal plants have potential role to promote plant growth through different mechanisms. However, the biological and ecological roles of fungal endophytes still totally unexplored. In this study, three different fungal endophytes were isolated from the medicinal plant of Asclepias sinaica and identified as Penicillium chrysogenum Pc_25, Alternaria alternata Aa_27 and the third fungal strain was described as sterile hyphae Sh_26. It was recorded that, these endophytes had various ability to produce several extracellular enzymes including amylase, pectinase, cellulase, gelatinase, xylanase and tyrosinase. Their antimicrobial activities against different specific test organisms were investigated as well. In addition, both endophyte isolates i.e. Sh_26 and Aa_27 were found to promote root growth higher than Pc_25 and control treatments. These fungal isolates had a considerable impact on plant growth parameters including root elongation as a result of ammonia and IAA production. ª 2015 Production and hosting by Elsevier B.V. on behalf of Faculty of Agriculture, Ain Shams University.

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Section: Discussionmentioning

confidence: 63%

Biotechnological applications of fungal endophytes associated with medicinal plant Asclepias sinaica (Bioss.)

Fouda

Hassan

Eid

et al. 2015

Annals of Agricultural Sciences

208

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“…Like this study, these limited data showed that seeds had significantly fewer fungal endophyte taxa, lower diversity and different assemblage to other tissues/organs. For example, only 4–12 species were recovered from seven plant oil seeds [46]. In endophytes from the seed of seven oil plants, only one plant had a Shannon’s index over 2.5 [46], and only two with Simpsons indices over 0.5 [46].…”

Section: Discussionmentioning

confidence: 99%

“…For example, only 4–12 species were recovered from seven plant oil seeds [46]. In endophytes from the seed of seven oil plants, only one plant had a Shannon’s index over 2.5 [46], and only two with Simpsons indices over 0.5 [46]. The seed of coffee’s ( Coffea arabica ) diversity index (Fisher’s alpha) was far less than that of stem and leaf (3.6 vs. 17.0 and 31.1, respectively) [47].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Endophytic Fungi from Acer ginnala Maxim. in an Artificial Plantation: Media Effect and Tissue-Dependent Variation

Jing

2012

PLoS ONE

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The community of endophytic fungi associated with Acer ginnala, a common tree in northeastern China, was investigated. Four media, PDA, Czapek’s, WA and Sabouraud’s, were used to inoculate explants from seeds, annual twigs and perennial twigs (xylem and bark). Media strongly affected the isolated species number, but not colonization frequency (CF) or isolation frequency (IF). To investigate media effect further, a Principal Component Analysis (PCA) was done. As a result, two components accounted for 86.502% of the total variance were extracted. These two components were named as PDA-determined factor (accounted for 45.139% of the total variance) and Czapek’s-determined factor (accounted for 41.363% of the total variance), respectively. This result suggested that only two media, PDA and Czapek’s, could be used instead of all four media in this study without affecting the isolation results significantly. In total, ten taxa were isolated in this study. Alternaria sp., Phomopsis sp., Neurospora sp. and Phoma sp. were dominant endophytes while Pleosporales Incertae Sedis sp., Cladosporium sp., Trichoderma sp. and Epicoccum sp. were rare taxa. Different tissues/organs had different endophyte assemblages. All tissue/organ pairs had low Bray-Curtis indices (<0.3) except for bark and annual twigs (0.63). Compared to perennial twigs, annual twigs had a lower taxon number, lower isolate number, lower endophyte dominance and diversity indices. Seeds had distinct assemblage, lower similarity and similar low diversity indices to annual twigs. These results suggested that tissue type determines the endophyte assemblage while age determines the diversity.

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“…The cellulase and pectinase activity of endophytic fungi isolated from oil-bearing seeds and medicinal plants has been described by Venkatesagowda et al (2012) and Sunitha et al (2013). Extracellular hydrolytic enzymes produced by fungal endophytes, particularly cellulase and pectinase, provide host-derived nutrients and resistance against pathogenic microbial infection.…”

Section: Discussionmentioning

confidence: 97%

Extracellular enzymatic profiles and taxonomic identification of endophytic fungi isolated from four plant species

et al. 2016

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Plants of medicinal and economic importance have been studied to investigate the presence of enzyme-producing endophytic fungi. The characterization of isolates with distinct enzyme production potential may identify suitable alternatives for specialized industry. At Universidade Estadual de Maringá Laboratory of Microbial Biotechnology, approximately 500 isolates of endophytic fungi have been studied over the last decade from various host plants, including medicinally and economically important species, such as Luehea divaricata (Martius et Zuccarini), Trichilia elegans A. Juss, Sapindus saponaria L., Piper hispidum Swartz, and Saccharum spp. However, only a fraction of these endophytes have been identified and evaluated for their biotechnological application, having been initially grouped by morphological characteristics, with at least one representative of each morphogroup tested. In the current study, several fungal strains from four plants (L. divaricata, T. elegans, S. saponaria, and Saccharum spp) were identified by ribosomal DNA typing and evaluated semi-quantitatively for their enzymatic properties, including amylase, cellulase, pectinase, and protease activity. Phylogenetic analysis revealed the presence of four genera of endophytic fungi (Diaporthe, Saccharicola, Bipolaris, and Phoma) in the plants examined. According to enzymatic tests, 62% of the isolates exhibited amylase, approximately 93% cellulase, 50% pectinase, and 64% protease activity. Our results verified that the composition and abundance of endophytic fungi differed between the plants tested, and that these endophytes are a potential enzyme production resource of commercial and biotechnological value.

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Diversity of plant oil seed-associated fungi isolated from seven oil-bearing seeds and their potential for the production of lipolytic enzymes

Cited by 52 publications

References 35 publications

Biotechnological applications of fungal endophytes associated with medicinal plant Asclepias sinaica (Bioss.)

Biotechnological applications of fungal endophytes associated with medicinal plant Asclepias sinaica (Bioss.)

Characterization of Endophytic Fungi from Acer ginnala Maxim. in an Artificial Plantation: Media Effect and Tissue-Dependent Variation

Extracellular enzymatic profiles and taxonomic identification of endophytic fungi isolated from four plant species

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