Degree of DNA methylation in chicory (Cichorium intybus L.): influence of plant age and vernalization

Demeulemeester, M. A. C.; Stallen, Nico Van; Proft, M.P. De

doi:10.1016/s0168-9452(99)00010-2

Cited by 72 publications

(40 citation statements)

References 13 publications

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“…More recently, Whole Genome Bisulfite Sequencing in three Brassicaceae species found lower methylation levels in roots than shoots, with changes between the two tissues being more frequent at repetitive sites and transposons, although across-species variation in cytosine methylation patterns was larger [3]. Here, we found that, in E. cicutarium, methylation was also lower in roots than in above ground leaves (see [47,50] for similar results in chicory and cotton, respectively). Scarcity of studies, disparity of the analytical methods that were used to quantify cytosine methylation, and some contrasting results between the few species analyzed in more detail (see e.g., [51]) precludes us to ascertain whether, apart from a reduced methylation level in endosperm, methylation varies among plant tissues in some definite way [52].…”

Section: Discussionsupporting

confidence: 64%

“…Some early epigenetic studies reported differences in global cytosine methylation among specific tissues/organs, as well as along tissue maturation stages (e.g., [1,[46][47][48][49] and references therein). More recently, Whole Genome Bisulfite Sequencing in three Brassicaceae species found lower methylation levels in roots than shoots, with changes between the two tissues being more frequent at repetitive sites and transposons, although across-species variation in cytosine methylation patterns was larger [3].…”

Section: Discussionmentioning

confidence: 99%

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Tissue-Specific Response to Experimental Demethylation at Seed Germination in the Non-Model Herb Erodium cicutarium

et al. 2017

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Experimental alteration of DNA methylation is a suitable tool to infer the relationship between phenotypic and epigenetic variation in plants. A detailed analysis of the genome-wide effect of demethylating agents, such as 5-azacytidine (5azaC), and zebularine is only available for the model species Arabidopsis thaliana, which suggests that 5azaC may have a slightly larger effect. In this study, global methylation estimates obtained by high-performance liquid chromatography (HPLC) analyses were conducted to investigate the impact of 5azaC treatment on leaf and root tissue in Erodium cicutarium (Geraniaceae), which is an annual herb native to Mediterranean Europe that is currently naturalized in all continents, sometimes becoming invasive. We used seeds collected from two natural populations in SE Spain. Root tissue of the second generation (F2) greenhouse-grown seedlings had a significantly lower global cytosine methylation content than leaf tissue (13.0 vs. 17.7% of all cytosines). Leaf tissue consistently decreased methylation after treatment, but the response of root tissue varied according to seed provenance, suggesting that genetic background can mediate the response to experimental demethylation. We also found that both leaf number and leaf length were reduced in treated seedlings supporting a consistent phenotypic effect of the treatment regardless of seedling provenance. These findings suggest that, although the consequences of experimental demethylation may be tissue-and background-specific, this method is effective in altering early seedling development, and can thus be useful in ecological epigenetic studies that are aiming to investigate the links between epigenetic and phenotypic variation in non-model plant species.

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Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Tissue-Specific Response to Experimental Demethylation at Seed Germination in the Non-Model Herb Erodium cicutarium

et al. 2017

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“…Then the same effects were reported in winter wheat (Brock andDavidson 1994, Yong et al 2003) and Cichorium intybus (Demeulemeester et al 1999). All these plants had to be exposed to a low temperature to be induced to flower, and DNA demethylation was considered to be involved in the regulation mechanism of vernalization.…”

Section: Discussionsupporting

confidence: 60%

“…This means that the present experimental system may be useful to study the regulation mechanism of the flowering-related genes in P. frutescens. After the start of 5-azacytidine treatment Comparison of flowering induced by 5-azacytidine and that induced by SD 5-Azacytidine effectively induced flowering even when applied to the shoot apical meristem of P. frutescens seedlings (Table 4) as compared with the previous works in which 5-azacytidine was treated to seeds (Sano et al 1990, Burn et al 1993, Brock and Davidson 1994, Finnegan et al 1998a, Demeulemeester et al 1999. As reported by Zeevaart (1958), P. frutescens seedlings respond to SD treatment only after the fourth leaf pair expanded.…”

Section: Discussionmentioning

confidence: 58%

Flowering induced by 5‐azacytidine, a DNA demethylating reagent in a short‐day plant, Perilla frutescens var. crispa

Kondo

Ozaki

Itoh

et al. 2006

Physiologia Plantarum

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Treatment with 5-azacytidine, a DNA demethylating reagent, induced flowering in Perilla frutescens (L.) Britton var. crispa (Thunb. ex Murray) Decne. ex L. H. Bailey, an absolute short-day plant under long days. The 5-azacytidine treatment induced slight suppression of vegetative growth but had no obvious effect on any other phenotypes. The Southern hybridization analysis of the genomic DNA isolated from the leaves of 5-azacytidine-treated plants and digested with restriction enzyme, methylation-insensitive Msp I or methylation-sensitive Hpa II with P. frutescens 25S-18S rDNA intergenic spacer probe indicated that the 5-azacytidine treatment caused demethylation of the genomic DNA. The 5-azacytidine-induced flowering was delayed as compared with the short day-induced flowering. Flowers were formed even at the lower nodes which had not been directly treated with 5-azacytidine. The results suggest that DNA demethylation induced flowering by inducing the production of a transmissible flowering stimulus in P. frutescens.

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“…Its glycosylase activity removes the 5-methylcytosine base, and its lyase activity nicks the DNA backbone at the abasic site by α , ß elimination mechanism (Agius et al 2006;Morales-Ruiz et al 2006). Methylation status dynamically changes depending upon cell type, developmental stages and even environmental conditions (Demeulemeester et al 1999;Steward et al 2002;Chakrabarty et al 2003;Wada et al 2004). First, because DNA methylation in nucleosomes induces alteration of gene expression by changing chromatin structures; vast demethylation may serve as a common switch for many genes that are simultaneously controlled upon environmental cues.…”

Section: Introductionmentioning

confidence: 99%

Regulation of Abiotic Stress Response Through NtROS2a-mediated Demethylation in Tobacco

Lee¹,

Choi²,

Niño³

et al. 2015

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT DNA methylation is a common and rapid evolving epigenetic mechanism among higher eukaryotic organisms including cytosine residues in CG and CNG sequences. NtROS2a gene isolated from tobacco plants (Nicotiana tabacum cv Xanthi nc) contains DNA glycosylase domain with significant similarities to Arabidopsis ROS1 and DME at the C-terminus. Purified NtROS2a protein gene is 5,879 bp long and is designated as Nicotiana tabacum Repressor Of Silencing (NtROS: accession numbers; AB281588).RT-PCR analysis initially revealed the adaptive response of the lines overexpressing the gene in tobacco to various abiotic stresses. This was validated when phenotypic evaluation to cold, salt, simulated drought and osmotic stress tolerance was performed. Reduction in leaf wilting rate against high salinity treatment is remarkable in transgenic plants which showed at least 50% less wilting than the WT. Moreover, about 70% wilt rate difference was noted between the transgenic and WT in H2O2 treatment. This study showed that NtROS2a gene encoding DNA glycosylase which caused demethylation could regulate adaptive response of tobacco to various abiotic stress conditions.

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Degree of DNA methylation in chicory (Cichorium intybus L.): influence of plant age and vernalization

Cited by 72 publications

References 13 publications

Tissue-Specific Response to Experimental Demethylation at Seed Germination in the Non-Model Herb Erodium cicutarium

Tissue-Specific Response to Experimental Demethylation at Seed Germination in the Non-Model Herb Erodium cicutarium

Flowering induced by 5‐azacytidine, a DNA demethylating reagent in a short‐day plant, Perilla frutescens var. crispa

Regulation of Abiotic Stress Response Through NtROS2a-mediated Demethylation in Tobacco

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