Dehalogenation of chlorinated dioxins by an anaerobic microbial consortium from sediment

Beurskens, J. E. M.; Wolf, J. de; Toussaint, Erel; Steen, Jan M.D. van der; Slot, Pieter C.; Commandeur, L.C.M.; Parsons, John R.

doi:10.1002/etc.5620140603

Cited by 91 publications

(57 citation statements)

References 42 publications

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“…Thus, the complete dechlorination of PCDD/Fs seemingly took place in the microcosm. This observation contrasts with those in previous studies of dechlorinating microbial consortia, in which PCDD/Fs decreased with the significant accumulation of less-chlorinated DD and DF (1,9,11,12,15,65). The discrepancy between the previous results and ours might be due to differences in the conditions used for dechlorination.…”

Section: Discussioncontrasting

confidence: 57%

Phylogenetic Characterization of a Polychlorinated-Dioxin- Dechlorinating Microbial Community by Use of Microcosm Studies

Yoshida

Takahashi

Hiraishi

2005

Appl Environ Microbiol

133

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Microcosms capable of reductive dechlorination of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/Fs) were constructed in glass bottles by seeding them with a polluted river sediment and incubating them anaerobically with an organic medium. All of the PCDD/F congeners detected were equally reduced without the accumulation of significant amounts of less-chlorinated congeners as the intermediate or end products. Alternatively, large amounts of catechol and salicylic acid were produced in the upper aqueous phase. Thus, the dechlorination of PCDD/Fs and the oxidative degradation of the dechlorinated products seemed to take place simultaneously in the microcosm. Denaturing gel gradient electrophoresis and clone library analyses of PCR-amplified 16S rRNA genes from the microcosm showed that members of the phyla Firmicutes, Proteobacteria, and Bacteroidetes predominated. A significant number of Chloroflexi clones were also detected. Quantitative real-time PCR with specific primer sets showed that the 16S rRNA genes of a putative dechlorinator, "Dehalococcoides," and its relatives accounted for 0.1% of the total rRNA gene copies of the microcosm. Most of the clones thus obtained formed a cluster distinct from the typical "Dehalococcoides" group. Quinone profiling indicated that ubiquinones accounted for 18 to 25% of the total quinone content, suggesting the coexistence and activity of ubiquinone-containing aerobic bacteria. These results suggest that the apparent complete dechlorination of PCDD/Fs found in the microcosm was due to a combination of the dechlorinating activity of the "Dehalococcoides"-like organisms and the oxidative degradation of the dechlorinated products by aerobic bacteria with aromatic hydrocarbon dioxygenases.

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Section: Discussioncontrasting

confidence: 57%

Phylogenetic Characterization of a Polychlorinated-Dioxin- Dechlorinating Microbial Community by Use of Microcosm Studies

Yoshida

Takahashi

Hiraishi

2005

Appl Environ Microbiol

133

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“…Studies on the microbial reductive dehalogenation of PCDD/Fs as well as of PCBs started appearing in the 1990's, using anaerobic sediment microcosms and mixed cultures 2,3,13,14,19,20,26,47,72,127) , although no phylogenetic information about PCDD/F-dechlorinating enrichment cultures had been available until the discovery of the ability of "Dehalococcoides" to dechlorinate PCDD/Fs. In 2003, Bunge et al 25) reported that "Dehalococcoides" sp.…”

Section: Pcdd/f-dehalogenating Bacteria and Consortiamentioning

confidence: 99%

Biodiversity of Dehalorespiring Bacteria with Special Emphasis on Polychlorinated Biphenyl/Dioxin Dechlorinators

Hiraishi

2008

Microb. Environ.

103

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A wide variety of haloorganic compounds undergo reductive dehalogenation by certain anaerobic microorganisms. Metabolic reductive dehalogenation is coupled with energy-conserving respiratory electron transport in which a halogenated compound is used as the terminal electron acceptor, the biological process called dehalorespiration or halorespiration. Dehalorespiring bacteria may play important roles in the geochemical cycle with organohalogens in nature and have great promise in their application to the bioremediation of haloorganic contaminants derived from anthropogenic sources. During the past decade, a number of dehalorespiring microorganisms, including a unique group of strictly dehalorespiring bacteria, "Dehalococcoides", have been isolated and characterized at phylogenetic, physiologic, and genetic levels. Also, new perspectives of dehalorespiring bacteria have emerged based on information about genomics and molecular microbial ecology. This review article focuses on up-to-date knowledge of the biodiversity of dehalorespiring bacteria and reductively dehalogenating microbial consortia with special emphasis on those capable of transforming polychlorinated biphenyls and dioxins.

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“…(3) Biodegradation effect. Some literatures (Adriaens and Grbic-Galic, 1994;Beurskens et al, 1995) had reported anaerobic biodegradation for some PCDD and PCDF congeners in sediments. The declining dioxins levels in this study were mainly due to the dilution effect of the flood.…”

Section: Declining Pcdd/f Levels In Sediments With Temporal Trendsmentioning

confidence: 99%

Declining polychlorinated dibenzo-p-dioxins and dibenzofurans levels in the sediments from Dongting Lake in China

et al. 2008

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a b s t r a c tDongting Lake is the second largest freshwater lake in China. Technical sodium pentachlorophenate (Na-PCP) had been sprayed since 1960s to control the spread of snailborne schistosomiasis up to the middle of 1990s. As one of the by-products of Na-PCP, polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) may enter the environment. It has been reported that the concentrations of PCDD/Fs in sediments in Dongting Lake were 130-891 pg I-TEQ g À1 in 1995. High toxicity of sediment to exposed microorganisms and fish may be risk on environment. In order to determine the present levels of the contamination, eight sediment samples from Dongting Lake were analyzed for PCDD/Fs using HRGC-HRMS. Total I-TEQ values for these samples were at a ranged of 0.7-11 pg g À1, with a mean value of 4.5 pg g À1 . The results show that PCDD/Fs concentrations of contemporary sediment have declined since 1995. Certain PCDD/F congeners as well as OCDD, typical for Na-PCP, predominated in lake sediment indicating that the using of PCP-Na is the main source of PCDD/Fs in the lake. These variations in levels can reflect changes in PCDD/F sources to the environment over time.

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Dehalogenation of chlorinated dioxins by an anaerobic microbial consortium from sediment

Cited by 91 publications

References 42 publications

Phylogenetic Characterization of a Polychlorinated-Dioxin- Dechlorinating Microbial Community by Use of Microcosm Studies

Phylogenetic Characterization of a Polychlorinated-Dioxin- Dechlorinating Microbial Community by Use of Microcosm Studies

Biodiversity of Dehalorespiring Bacteria with Special Emphasis on Polychlorinated Biphenyl/Dioxin Dechlorinators

Declining polychlorinated dibenzo-p-dioxins and dibenzofurans levels in the sediments from Dongting Lake in China

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