Dehalogenimonas lykanthroporepellens gen. nov., sp. nov., a reductively dehalogenating bacterium isolated from chlorinated solvent-contaminated groundwater

Moe, William M.; Yan, Jun; Nobre, M. Fernanda; Costa, Milton S. da; Rainey, Fred A.

doi:10.1099/ijs.0.011502-0

Cited by 165 publications

(141 citation statements)

References 31 publications

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“…Limited dechlorination activity of Dehalococcoides and related corrinoid-auxotroph, organohalide-respiring Chloroflexi (e.g. Dehalogenimonas [38,39], 'Dehalobium' [40]) is regarded as a major constraint to achieve detoxification at sites contaminated with chlorinated contaminants. Hence, elucidating the nutritional requirements and ecophysiologies of organohalide-respiring Chloroflexi in natural and contaminated environments will help develop predictive understanding and new strategies for stimulating in situ reductive dechlorination activity.…”

Section: Discussionmentioning

confidence: 99%

Guided cobalamin biosynthesis supports Dehalococcoides mccartyi reductive dechlorination activity

Yan

Yang

et al. 2013

Phil. Trans. R. Soc. B

132

101

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Dehalococcoides mccartyi strains are corrinoid-auxotrophic Bacteria and axenic cultures that require vitamin B 12 (CN-Cbl) to conserve energy via organohalide respiration. Cultures of D. mccartyi strains BAV1, GT and FL2 grown with limiting amounts of 1 µg l −1 CN-Cbl quickly depleted CN-Cbl, and reductive dechlorination of polychlorinated ethenes was incomplete leading to vinyl chloride (VC) accumulation. In contrast, the same cultures amended with 25 µg l −1 CN-Cbl exhibited up to 2.3-fold higher dechlorination rates, 2.8–9.1-fold increased growth yields, and completely consumed growth-supporting chlorinated ethenes. To explore whether known cobamide-producing microbes supply Dehalococcoides with the required corrinoid cofactor, co-culture experiments were performed with the methanogen Methanosarcina barkeri strain Fusaro and two acetogens, Sporomusa ovata and Sporomusa sp. strain KB-1, as Dehalococcoides partner populations. During growth with H 2 /CO 2 , M. barkeri axenic cultures produced 4.2 ± 0.1 µg l −1 extracellular cobamide (factor III), whereas the Sporomusa cultures produced phenolyl- and p -cresolyl-cobamides. Neither factor III nor the phenolic cobamides supported Dehalococcoides reductive dechlorination activity suggesting that M. barkeri and the Sporomusa sp. cannot fulfil Dehalococcoides ' nutritional requirements. Dehalococcoides dechlorination activity and growth occurred in M. barkeri and Sporomusa sp. co-cultures amended with 10 µM 5′,6′-dimethylbenzimidazole (DMB), indicating that a cobalamin is a preferred corrinoid cofactor of strains BAV1, GT and FL2 when grown with chlorinated ethenes as electron acceptors. Even though the methanogen and acetogen populations tested did not produce cobalamin, the addition of DMB enabled guided biosynthesis and generated a cobalamin that supported Dehalococcoides ' activity and growth. Guided cobalamin biosynthesis may offer opportunities to sustain and enhance Dehalococcoides activity in contaminated subsurface environments.

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Section: Discussionmentioning

confidence: 99%

Guided cobalamin biosynthesis supports Dehalococcoides mccartyi reductive dechlorination activity

Yan

Yang

et al. 2013

Phil. Trans. R. Soc. B

132

101

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“…No similar phylotype of Ced_B01 has been detected at any other alkaline or serpentinizing environments. The closest isolate, with 82% of 16S rRNA gene identity, is Dehalogenimonas lykanthroporepellens BL-DC-9, which is capable of reductive dehalogenation, a variety of polychlorinated alkanes (24). Although the phylotype Ced_B01 is most closely related to the family Dehalococcoidetes, it does not appear to be a member of this family.…”

Section: Taxonomic Comparison Of Dominant Phylotypes In the Cedars Withmentioning

confidence: 99%

Microbial diversity in The Cedars, an ultrabasic, ultrareducing, and low salinity serpentinizing ecosystem

Suzuki

Ishii

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

113

204

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The Cedars, in coastal northern California, is an active site of peridotite serpentinization. The spring waters that emerge from this system feature very high pH, low redox potential, and low ionic concentrations, making it an exceptionally challenging environment for life. We report a multiyear, culture-independent geomicrobiological study of three springs at The Cedars that differ with respect to the nature of the groundwater feeding them. Within each spring, both geochemical properties and microbial diversity in all three domains of life remained stable over a 3-y period, with multiple samples each year. Between the three springs, however, the microbial communities showed considerable differences that were strongly correlated with the source of the serpentinizing groundwater. In the spring fed solely by deep groundwater, phylum Chloroflexi, class Clostridia, and candidate division OD1 were the major taxa with one phylotype in Euryarchaeota. Less-abundant phylotypes include several minor members from other candidate divisions and one phylotype that was an outlier of candidate division OP3. In the springs fed by the mixture of deep and shallow groundwater, organisms close to the Hydrogenophaga within Betaproteobacteria dominated and coexisted with the deep groundwater community members. The shallow groundwater community thus appears to be similar to those described in other terrestrial serpentinizing sites, whereas the deep community is distinctly different from any other previously described terrestrial serpentinizing community. These unique communities have the potential to yield important insights into the development and survival of life in these early-earth analog environments.biodiversity | extremophile | alkaliphile | small subunit rRNA | hydrogen

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“…The genome of Dehalococcoides mccartyi (formerly ethenogenes) strain 195 [65] harbours 17 different rdhA genes [14], while strain CBDB1 0 s genome contains 32 rdhAs [13]. The genome of Dehalogenimonas lykanthroporepellens strain BL-DC-9, the only sequenced Dehalogenimonas, contains 19 rdhA genes [17,66]. The presence of multiple rdhA genes is also a feature in the Dehalobacter genus, with up to 24 complete putative rdhA genes within sequenced Dehalobacter genomes [21,22,67].…”

Section: Reductive Dehalogenasesmentioning

confidence: 99%

Overview of organohalide-respiring bacteria and a proposal for a classification system for reductive dehalogenases

Hug

Maphosa

Leys

et al. 2013

Phil. Trans. R. Soc. B

271

277

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Organohalide respiration is an anaerobic bacterial respiratory process that uses halogenated hydrocarbons as terminal electron acceptors during electron transport-based energy conservation. This dechlorination process has triggered considerable interest for detoxification of anthropogenic groundwater contaminants. Organohalide-respiring bacteria have been identified from multiple bacterial phyla, and can be categorized as obligate and non-obligate organohalide respirers. The majority of the currently known organohalide-respiring bacteria carry multiple reductive dehalogenase genes. Analysis of a curated set of reductive dehalogenases reveals that sequence similarity and substrate specificity are generally not correlated, making functional prediction from sequence information difficult. In this article, an orthologue-based classification system for the reductive dehalogenases is proposed to aid integration of new sequencing data and to unify terminology.

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Dehalogenimonas lykanthroporepellens gen. nov., sp. nov., a reductively dehalogenating bacterium isolated from chlorinated solvent-contaminated groundwater

Cited by 165 publications

References 31 publications

Guided cobalamin biosynthesis supports Dehalococcoides mccartyi reductive dechlorination activity

Guided cobalamin biosynthesis supports Dehalococcoides mccartyi reductive dechlorination activity

Microbial diversity in The Cedars, an ultrabasic, ultrareducing, and low salinity serpentinizing ecosystem

Overview of organohalide-respiring bacteria and a proposal for a classification system for reductive dehalogenases

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