Dehydration Impairs Physical Growth and Cognitive Development in Young Mice

Kim, Chong-Su; Chun, Woo Young; Shin, Dong Wan

doi:10.3390/nu12030670

Cited by 9 publications

(12 citation statements)

References 36 publications

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“…In a previous study, our group confirmed that drinking water intake as part of the diet had an important influence on cognitive development by showing that dehydration during infancy resulted in abnormal cognitive development in infant mice [ 3 ]. The present study aimed to determine whether changes in the gut microbiota were associated with cognitive decline in dehydrated mice through the gut-brain axis.…”

Section: Introductionmentioning

confidence: 86%

“…Adequate water intake is essential for the body’s normal biological and physiological functions [ 1 , 2 ]. Insufficient drinking water consumption during the developmental period is related to various health outcomes, including altered hippocampal gene expression related to cognitive development, abnormal physical growth, and renal development [ 3 , 4 ]. Moreover, not only the water quantity consumed but also the source of drinking water has been considered for their health benefits.…”

Section: Introductionmentioning

confidence: 99%

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Gut microbiota and cognitive development in infant mice: Quantity and source of potable water

Kim

Shin

2023

PLoS ONE

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Not only the water quantity consumed but also the source of drinking water has been considered for their health benefits, but there is limited evidence. We aimed to determine whether the amount and type of drinking water affect physiological and biological functions, including brain function, by confirming how it affects gut microbiota which has an important regulatory role in host physiology. Three-week-old infant mice were subjected to 1) a water restriction experiment (control group, ad libitum consumption of distilled water; dehydration group, time-limited access to distilled water [15 min/day]) and 2) different water source experiment (distilled water, purified water, spring water, and tap water groups). The gut microbiota and cognitive development were analyzed using the 16S ribosomal ribonucleic acid sequencing method and the Barnes maze, respectively. The relative abundance of Firmicutes and Bacteroidetes and the Firmicutes-to-Bacteroidetes ratio (F/B ratio) changed depending on age (juveniles vs. infants). Insufficient water intake reversed these developmental changes, showing that the relative abundances of Bacteroidetes and Firmicutes and the F/B ratio in dehydrated juvenile mice were similar to those in normal infant mice. Additionally, clustering analysis revealed no significant differences in the intestinal flora in the mice from the different drinking water sources; however, dehydration significantly altered the composition of the genera compared to the other water source groups wherein water was provided ad libitum. Moreover, cognitive development was significantly disrupted by insufficient water intake, although the type of drinking water had no significant influence. Cognitive decline, measured by relative latency, was positively associated with the relative abundance of unclassified Erysipelotrichaceae that were in significantly high relative abundance in the dehydration group. These results suggest that the water quantity consumed, rather than the mineral content of drinking water, is imperative for shaping the early gut microbiota associated with cognitive development during infancy.

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Section: Introductionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Gut microbiota and cognitive development in infant mice: Quantity and source of potable water

Kim

Shin

2023

PLoS ONE

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“…Neurobehavioral tests were conducted in the Animal Behavior Facility at the University of Occupational and Environmental Health during the light phase (between 09:00–13:00). Spatial memory was evaluated using the Barnes maze test, a standardized method for assessing spatial memory in rats and mice [ 20 ]. The utilized maze comprised a circular platform (92 cm in diameter) with 20 equally spaced holes around the periphery.…”

Section: Methodsmentioning

confidence: 99%

Deletion of Wnt10a Is Implicated in Hippocampal Neurodegeneration in Mice

et al. 2022

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The hippocampus plays an important role in maintaining normal cognitive function and is closely associated with the neuropathogenesis of dementia. Wnt signaling is relevant to neuronal development and maturation, synaptic formation, and plasticity. The role of Wnt10a in hippocampus-associated cognition, however, is largely unclear. Here, we examined the morphological and functional alterations in the hippocampus of Wnt10a-knockout (Wnt10a-/-) mice. Neurobehavioral tests revealed that Wnt10a-/- mice exhibited spatial memory impairment and anxiety-like behavior. Immunostaining and Western blot findings showed that the protein expressions of β-catenin, brain-derived neurotrophic factor, and doublecortin were significantly decreased and that the number of activated microglia increased, accompanied by amyloid-β accumulation, synaptic dysfunction, and microglia-associated neuroinflammation in the hippocampi of Wnt10a-/- mice. Our findings revealed that the deletion of Wnt10a decreased neurogenesis, impaired synaptic function, and induced hippocampal neuroinflammation, eventually leading to hippocampal neurodegeneration and memory deficit, possibly through the β-catenin signaling pathway, providing a novel insight into preventive approaches for hippocampus-dependent cognitive impairment.

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“…Water restriction. Chronic water restriction was induced for 4 weeks as previously described 58 . Briefly, animals in the RES group were given limited access to water; a water bottle was given to animals for 15 min at the same time each day during the experimental period.…”

Section: Methodsmentioning

confidence: 99%

Renal transcriptome profiles in mice reveal the need for sufficient water intake irrespective of the drinking water type

Shon

Park

Lee

et al. 2022

Sci Rep

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This study sought to characterize the impact of long-term dehydration in terms of physiological and biochemical parameters, as well as renal transcriptomes. Furthermore, we assessed whether consumption of specific types of water elicit more beneficial effects on these health parameters. To this end, C57BL/6 mice were either provided water for 15 min/day over 2 and 4 weeks (water restricted; RES), or ad libitum access to distilled (CON), tap, spring, or purified water. Results show that water restriction decreases urine output and hematocrit levels while increasing brain vasopressin mRNA levels in RES mice compared to control mice (CON). Meanwhile, blood urea nitrogen and creatinine levels were higher in the RES group compared to the CON group. Kidney transcriptome analysis further identified kidney damage as the most significant biological process modulated by dehydration. Mechanistically, prolonged dehydration induces kidney damage by suppressing the NRF2-signaling pathway, which targets the cytoprotective defense system. However, type of drinking water does not appear to impact physiological or blood biochemical parameters, nor the renal transcriptome profile, suggesting that sufficient water consumption is critical, irrespective of the water type. Importantly, these findings also inform practical action for environmental sustainability by providing a theoretical basis for reducing bottled water consumption.

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Dehydration Impairs Physical Growth and Cognitive Development in Young Mice

Cited by 9 publications

References 36 publications

Gut microbiota and cognitive development in infant mice: Quantity and source of potable water

Gut microbiota and cognitive development in infant mice: Quantity and source of potable water

Deletion of Wnt10a Is Implicated in Hippocampal Neurodegeneration in Mice

Renal transcriptome profiles in mice reveal the need for sufficient water intake irrespective of the drinking water type

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