Dehydration of 2,3-Butanediol to 1,3-Butadiene and Methyl Ethyl Ketone: Modeling, Numerical Analysis and Validation Using Pilot-Scale Reactor Data

Song, Daesung; Cho, Sung Yong; Vu, Toan-Thang; Duong, Hoang-Phi-Yen; Kim, Eunkyu

doi:10.3390/catal11080999

Cited by 7 publications

(4 citation statements)

References 24 publications

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“…Many authors rank inulin as the most attractive substrate for microbial production of 2,3-BD [3,4,[36][37][38]. This opinion is based on the development of several very efficient processes engaging Klebsiella pneumoniae (belonging to the Risk 1 bacterial group).…”

Section: Discussionmentioning

confidence: 99%

“…According to a recent forecast, the global market for 2,3-butanediol (2,3-BD) will grow to USD 300 million by 2030 [1]. This incredibly high demand is due to the application of 2,3-BD in several industries, namely, food (as a flavoring ingredient), chemical (as a platform reagent in polymer synthesis), cosmetics and pharmaceuticals, and also as antifreeze and additive in oils and fuels [2][3][4][5][6]. The perspectives on the production of bio-based chemicals in the forthcoming 2022-2050 period include an increase in the demand for carbon for chemicals from 450 million metric tons (MMt) per annum in 2021 to 1000 MMt per annum in 2050 [7].…”

Section: Introductionmentioning

confidence: 99%

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Heterologous Expression of Inulinase Gene in Bacillus licheniformis 24 for 2,3-Butanediol Production from Inulin

et al. 2023

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Inulin is a renewable and cheap carbon source used in microbial fermentations. Bacillus licheniformis 24 is known as an excellent 2,3-butanediol (2,3-BD) producer from fructose; therefore, the cloning and expression of a robust heterologous inulinase could enhance its 2,3-BD production from inulin. The inu gene of Lacticaseibacillus paracasei DSM 23505 encoding fructan-β-fructosidase (EC 3.2.1.80) was chosen for the purpose. PCR fragments containing the complete inu (3.6 kb) and its truncated variant inu-tr (2.2 kb, lacking Big3 cell wall attachment domains) were cloned into Escherichia coli StellarTM and B. licheniformis 24. The high quality of the recombinant constructs was confirmed by restriction analysis, PCR, sequencing, and phenotypic tests. The results showed that the inulinase activity of B. licheniformis cells harboring the full-length inu variant (T26) was eightfold higher compared to the wild type, retaining cell wall attachment in the B. licheniformis host. In contrast, the truncated variant inu-tr (T14) showed mostly extracellular but weak activity, thus suggesting that the Big3 domains are also important for the enzyme’s function. During flask-batch fermentation of 100 g/L raw chicory flour (containing 90% inulin), T26 produced acetoin and 2,3-BD from inulin. Contrariwise, T14 and the wild type formed products only from the mono- and disaccharides naturally found in the chicory flour. In the fermenter, from 200 g/L of raw chicory flour, the recombinant T26 degraded approximately 140 g/L of the inulin. However, the final concentrations of the produced 2,3-BD and acetoin were 18.5 g/L and 8.2 g/L, respectively, because of the accumulation of unconverted sucrose. To conclude, further strain improvement is necessary to make the process efficient for obtaining 2,3-BD from inulin by simultaneous saccharification and fermentation (SSF).

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Heterologous Expression of Inulinase Gene in Bacillus licheniformis 24 for 2,3-Butanediol Production from Inulin

et al. 2023

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“…Such an example is the production of 2,3-butanediol (2,3-BD), with the most rapidly growing market [ 2 ], projected to reach USD 300 million by the end of 2030 [ 3 ]. It is used as a fuel additive, antifreeze, solvent, flavoring agent, platform chemical for polyesters, polyurethanes, and methacrylate synthesis, and for the production of rubber, drugs, ointments, and antiperspirants [ 4 , 5 , 6 , 7 ]. The possibility of the biotechnological synthesis of 2,3-BD dates back to 1906 when Harden and Walpole investigated its production by Klebsiella pneumoniae [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…The first pilot plant for the production of 2,3-BD was built, and the process was carried out via the microbial fermentation of the K. oxytoca and P. polymyxa strains. However, large-scale industrial production never took off as it turned out to be more cost-effective to produce 1,3-butadiene from petroleum [ 5 ]. In the 1970s, due to rising oil prices, interest in 2,3-BD biotechnology returned.…”

Section: Introductionmentioning

confidence: 99%

Influence of pH on Inulin Conversion to 2,3-Butanediol by Bacillus licheniformis 24: A Gene Expression Assay

Tsigoriyna,

Arsov,

Gergov

et al. 2023

IJMS

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2,3-Butanediol (2,3-BD) is an alcohol highly demanded in the chemical, pharmaceutical, and food industries. Its microbial production, safe non-pathogenic producer strains, and suitable substrates have been avidly sought in recent years. The present study investigated 2,3-BD synthesis by the GRAS Bacillus licheniformis 24 using chicory inulin as a cheap and renewable substrate. The process appears to be pH-dependent. At pH 5.25, the synthesis of 2,3-BD was barely detectable due to the lack of inulin hydrolysis. At pH 6.25, 2,3-BD concentration reached 67.5 g/L with rapid hydrolysis of the substrate but was accompanied by exopolysaccharide (EPS) synthesis. Since inulin conversion by bacteria is a complex process and begins with its hydrolysis, the question of the acting enzymes arose. Genome mining revealed that several glycoside hydrolase (GH) enzymes from different CAZy families are involved. Five genes encoding such enzymes in B. licheniformis 24 were amplified and sequenced: sacA, sacB, sacC, levB, and fruA. Real-time RT-PCR experiments showed that the process of inulin hydrolysis is regulated at the level of gene expression, as four genes were significantly overexpressed at pH 6.25. In contrast, the expression of levB remained at the same level at the different pH values at all-time points. It was concluded that the sacC and sacA/fruA genes are crucial for inulin hydrolysis. They encode exoinulinase (EC 3.2.1.80) and sucrases (EC 3.2.1.26), respectively. The striking overexpression of sacB under these conditions led to increased synthesis of EPS; therefore, the simultaneous production of 2,3-BD and EPS cannot be avoided.

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Dehydration of 2,3-Butanediol to 1,3-Butadiene and Methyl Ethyl Ketone: Modeling, Numerical Analysis and Validation Using Pilot-Scale Reactor Data

Cited by 7 publications

References 24 publications

Heterologous Expression of Inulinase Gene in Bacillus licheniformis 24 for 2,3-Butanediol Production from Inulin

Heterologous Expression of Inulinase Gene in Bacillus licheniformis 24 for 2,3-Butanediol Production from Inulin

Influence of pH on Inulin Conversion to 2,3-Butanediol by Bacillus licheniformis 24: A Gene Expression Assay

Advances and challenges in bio-based 2,3-BD downstream purification: A comprehensive review

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