Dehydroepiandrostenedione sulphate interferes in many direct immunoassays for testosterone

Middle, Jonathan

doi:10.1258/000456307780118082

Cited by 57 publications

(29 citation statements)

References 5 publications

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“…A direct E 1 S RIA method, similar to those employed in the DSL-5400 kit, was reported previously [20] but the interference of DHEAS in E 1 S determination is not provided in this paper. Interference of DHEAS in many direct immunoassays for testosterone in male and female plasma has also been reported previously, due to the considerably higher plasma levels of DHEAS than testosterone, associated with insufficient specificity of the antitestosterone antibody employed [21]. A very recent paper [22] comparing LC-MS/MS with the DSL-5400 kit also reported higher E 1 S using the kit and suggested the interference of DHEAS, but do not proved it.…”

Section: Discussionmentioning

confidence: 86%

Plasma estrone sulfate assay in men: Comparison of radioimmunoassay, mass spectrometry coupled to gas chromatography (GC–MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Giton¹,

Caron²,

Bérubé³

et al. 2010

Clinica Chimica Acta

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, et al.. Plasma estrone sulfate assay in men: Comparison of radioimmunoassay, mass spectrometry coupled to gas chromatography (GC-MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS).: Plasma estrone sulfate assay in men: comparison between four different assay methods. Clinica Chimica Acta, Elsevier, 2010, 411 (17-18) Acknowledgments:The authors thank Dr. Noah Hardy for editing the manuscript. Methods:We simultaneously assayed estrone sulfate in the plasma of one hundred men aged 30-50 years, according to LC-MS/MS, GC-MS after solvolysis of E 1 S, radioimmunoassay after a chromatographic purification step, and a direct RIA commercial kit.Results: Estrone sulfate plasma levels obtained with the first three methods were not significantly different. However, estrone sulfate levels measured by the direct RIA were threefold higher than those obtained by the first three methods. We showed that the excessively high estrone sulfate levels obtained with the direct RIA kit had two origins:interference by high dehydroepiandrosterone sulfate plasma levels in men, and estrone sulfate inaccurate low concentrations in the standards. Conclusion:The LC-MS/MS method can be considered as an optimum option for clinical laboratory. The GC-MS method requires solvolysis to estrone, but allows simultaneous unconjugated steroid measurement. RIA method, with chromatographic purification, is cumbersome, but less expensive. DSL-5400 kit yielded estrone sulfate plasma levels that were too high.Plasma estrone sulfate assay in men: comparison between four different assay methods 3

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Section: Discussionmentioning

confidence: 86%

Plasma estrone sulfate assay in men: Comparison of radioimmunoassay, mass spectrometry coupled to gas chromatography (GC–MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Giton¹,

Caron²,

Bérubé³

et al. 2010

Clinica Chimica Acta

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“…The extraction steps were discarded to streamline the process but the resultant direct methods, although convenient, could suffer significant interference from other steroids. For example, the most abundant serum steroid DHEAS (mM concentration) will interfere with IAs looking for low level androgens such as testosterone (!10 nM) (18). Before the introduction of large analyser systems, there was scope to change the kit supplier if the assay performed badly, but this is now virtually impossible because reagents are tied into the analyser and kits are not interchangeable between different manufacturer systems.…”

Section: Inter-assay Performance By Lc-ms/ms and Iamentioning

confidence: 99%

Mass spectrometry and immunoassay: how to measure steroid hormones today and tomorrow

Taylor¹,

Keevil²,

Huhtaniemi³

2015

European Journal of Endocrinology

260

157

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SummaryThe recent onslaught of mass spectrometry (MS) to measurements of steroid hormones, including demands that they should be the only acceptable method, has confused clinicians and scientists who have relied for more than 40 years on a variety of immunoassay (IA) methods in steroid hormone measurements. There is little doubt that MS methods with their superior specificity will be the future method of choice in many clinical and research applications of steroid hormone measurement. However, the majority of steroid measurements are currently, and will continue to be, carried out using various types of IAs for several reasons, including their technical ease, cost and availability of commercial reagents. Speedy replacement of all IAs with MS is an unrealistic and unnecessary goal, because the availability of MS measurements is limited by cost, need of expensive equipment, technical demands and lack of commercial applications. Furthermore, IAs have multiple well-known advantages that vindicate their continuing use. The purpose of this article is to elucidate the advantages and limitations of the MS and IA techniques from two angles, i.e. promotion of MS and defence of IA. The purpose of the text is to give the reader an unbiased view about the current state and future trends of steroid analysis and to help him/her choose the correct assay method to serve his/her diagnostic and research needs.

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“…Faster direct immunoassays replaced the assays with pre-treatment in clinical routine analysis within the last 25 years. Although these direct immunoassays are simple, rapid and cheap, they may lack in reliability, sensitivity and specificity especially in low concentration ranges [4][5][6]. At present, the use of LC-MS/MS becomes increasingly important in clinical laboratory diagnostics.…”

Section: Introductionmentioning

confidence: 99%

Preclinical challenges in steroid analysis of human samples

Ceglarek

Werner

Kortz

et al. 2010

The Journal of Steroid Biochemistry and Molecular Biology

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Dehydroepiandrostenedione sulphate interferes in many direct immunoassays for testosterone

Abstract: DHEAS interference in testosterone assays is a feature of many routine testosterone methods, and occurs in both male and female matrices.

Cited by 57 publications

References 5 publications

Plasma estrone sulfate assay in men: Comparison of radioimmunoassay, mass spectrometry coupled to gas chromatography (GC–MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Plasma estrone sulfate assay in men: Comparison of radioimmunoassay, mass spectrometry coupled to gas chromatography (GC–MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Mass spectrometry and immunoassay: how to measure steroid hormones today and tomorrow

Preclinical challenges in steroid analysis of human samples

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