Dehydroepiandrosterone administration prevents the oxidative damage induced by acute hyperglycemia in rats

Aragno, Manuela; Brignardello, Enrico; Tamagno, Elena; Gatto, V; Danni, Oliviero; Boccuzzi, Giuseppe

doi:10.1677/joe.0.1550233

Cited by 123 publications

(66 citation statements)

References 37 publications

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“…Furthermore, the renal levels of lipid peroxides, MDA, were significantly reduced in DHEA treated rats as compared to PCM intoxicated rats. Our results are in the same line with Aragno et al (1997;Boccuzzi et al, 1997) who reported that DHEA possesses antioxidant activity. Also, Kumar et al (2008) have shown that the protective effects of DHEA in various disease condition are attributed to its antioxidant, anti-lipidperoxidative, anti-inflammatory and thereby anti-aging actions.…”

Section: Discussionsupporting

confidence: 93%

Impact of Dehydroepiandrosterone in Prevention of Paracetamol Induced Nephrotoxicity in Rats

El-Karib¹

2014

American Medical Journal

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Paracetamol (PCM) overdose can cause nephrotoxicity with oxidative stress as one of the possible mechanisms mediating the event. However, Dehydroepiandrosterone (DHEA), the major secretory product of the human adrenal gland, has been shown to possess a multi-targeted antioxidant activity which is also effective against lipid peroxidation induced in various animal models and against various human disorders. In this study, the preventive effect of DHEA against PCM-induced nephrotoxicity was examined. Rats were divided into four groups containing 10 rats each, as follows: A control: Received normal saline, Vehicle treated: Received the vehicle (5% DMSO), PCM model (750 mg kg ), respectively, for 4 consecutive weeks. All treatment were given orally to animals. Our results show that co-treatment of DHEA with PCM prevented the PCM-induced nephrotoxicity and oxidative impairments of the kidney, as evidenced by a significantly reduced (p<0.05) level of serum creatinine, urea and BUN with parallel significant increases in serum protein, Cr clearance and kidneys weights. Furthermore, DHEA was able to induce a significant increment (p<0.05) of renal levels of reduced Glutathione (GSH) and activities of Superoxide Dismutase (SOD) and Glutathione Peroxidise (GPx). An effect that was accompanied with a significant decrease in renal lipid peroxides levels (MDA). The nephroprotective effects of DHEA was confirmed by a reduced intensity of renal cellular damage, as evidenced by histological findings. In conclusion, DHEA at a daily dose of 250 mg kg −1 has a protective role against PCM-induced nephrotoxicity in rats and the process is probably mediated through its antioxidant properties.

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Section: Discussionsupporting

confidence: 93%

Impact of Dehydroepiandrosterone in Prevention of Paracetamol Induced Nephrotoxicity in Rats

El-Karib¹

2014

American Medical Journal

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“…Similarly, oxidative damage in rat brain is increased by experimentally induced hyperglycemia (Aragno et al, 1997). It has been suggested that the modified oxidative state induced by chronic hyperglycemia may contribute to nervous tissue damage; free radical species impair the central nervous system, attacking neurons and schwann cells, and the peripheral nerves (Kawai et al, 1998;Kumar & Menon, 1998;Aragno, 2000).…”

Section: Resultsmentioning

confidence: 99%

Anthocephalus cadamba (Roxb.) Miq., Rubiaceae, extract shows hypoglycemic effect and eases oxidative stress in alloxan-induced diabetic rats

Alam¹,

Subhan²,

Chowdhury³

et al. 2011

Rev. bras. farmacogn.

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Abstract:The hydroethanolic extract of the flowering tops of Anthocephalus cadamba (Roxb.) Miq., Rubiaceae, a Bangladeshi medicinal plant, was studied for its potential hypoglycemic effect and antioxidant property in alloxan-induced diabetic rats. The extract induced significant reduction in serum glucose, and transaminases, e.g. aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatases (ALP), activities. Significant changes in the thiobarbituric acid reactive substances (TBARS), peroxidase and catalase levels during the experimental period were also observed. The results established that the hydroethanolic extract of the flowering tops of A. cadamba possesses hypoglycemic property and is able to protect liver and brain from oxidative damages caused by diabetes.

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“…These wide-ranging activities cannot fully be explained by activation of the androgen or estrogen receptors. Published data support the hypothesis that DHEA acts to limit lipid peroxidation, in vivo as well as in vitro (17)(18)(19)(20)(21)(22)(23). Since many of the diseases on which DHEA has a beneficial effect have been related to repeated oxidative insult (24), the antioxidant properties of DHEA might be expected to explain its effects.…”

Section: Introductionmentioning

confidence: 64%

“…DHEA has recently been reported to protect human LDL against lipid peroxidation by regenerating the endogenous vitamin E of LDL (22). However, the content of vitamin E and other antioxidants has been reported to remain unchanged in tissue homogenates of DHEA-treated rats (21).…”

Section: Discussionmentioning

confidence: 99%

Protective effect of dehydroepiandrosterone against lipid peroxidation in a human liver cell line

Gallo

Aragno

Gatto

et al. 1999

European Journal of Endocrinology

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Objective: Dehydroepiandrosterone (DHEA) is a widely studied steroid hormone with multi-functional properties. Reports suggest that some of the many activities of DHEA are due to its protective effect against lipid peroxidation. Nevertheless, the antioxidant properties of DHEA are still the subject of debate. The aim was to evaluate whether its two opposed effects on lipid peroxidation reported in the literature may be dependent on schedule and doses used. Methods: Chang liver cells, a line derived from normal human liver, were grown in media containing either no steroids (control) or DHEA at concentrations ranging from 0.1 mmol/l to 50 mmol/l. At specific times, cultures were halted and cells received a pro-oxidant stimulus (cumene (CuOOH) 0.5 mmol/l), at which time cell viability (by trypan blue staining and lactate dehydrogenase (LDH) release) and thiobarbituric acid reactive substances (TBARS) concentration (spectrophotometrical assay) were evaluated. Results: At concentrations ranging from 0.1 mmol/l to 1 mmol/l, DHEA protects Chang liver cells against lipid peroxidation and/or death induced by cumene. This effect disappears if the concentration is increased to 10 mmol/l; at higher concentrations (50 mmol/l) a pro-oxidant/cytotoxic effect of DHEA appears. Conclusions: DHEA exhibits two opposed effects on lipid peroxidation; depending on its concentration it acts either to limit or to induce oxidative stress. The threshold concentration at which the pro-oxidant activity of DHEA prevails is not far in excess of that having an antioxidant effect. Either effect of DHEA on lipid peroxidation is only evident after a 'lag-phase'.

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Dehydroepiandrosterone administration prevents the oxidative damage induced by acute hyperglycemia in rats

Cited by 123 publications

References 37 publications

Impact of Dehydroepiandrosterone in Prevention of Paracetamol Induced Nephrotoxicity in Rats

Impact of Dehydroepiandrosterone in Prevention of Paracetamol Induced Nephrotoxicity in Rats

Anthocephalus cadamba (Roxb.) Miq., Rubiaceae, extract shows hypoglycemic effect and eases oxidative stress in alloxan-induced diabetic rats

Protective effect of dehydroepiandrosterone against lipid peroxidation in a human liver cell line

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