Dekkera bruxellensis, a beer yeast that specifically bioconverts mogroside extracts into the intense natural sweetener siamenoside I

Encapsulation of β-Glucosidase within PVA Fibers by CCD-RSM-Guided Coelectrospinning: A Novel Approach for Specific Mogroside Sweetener Production

“…On the basis of the TLC results, it is quite obvious that S I was produced during the bioconversion process (Figure B), while the control group, S. cerevisiae BY4741 WT cells, first converted MG V to S I and MG IV as intermediates before proceeding to the final production of MG III E (Figure C). These results were similar to those of a previous study …”

“…The Db Exg1 enzyme from D. bruxellensis has been found to have S I production specificity from MG V . Nevertheless, the slow growth rate of this organism and its role in wine spoilage have hampered the production of S I through bioconversion .…”

“…To create a yeast strain with stable Db Exg1 secretion activity, the gene responsible for the enzyme could be obtained from the DbEXG1 expression vector using the PCR amplification method …”

“…After the plate was fully permeated with the developing solvent, the remaining solvent on the plate was allowed to evaporate, and the plate was sprayed with 10% (v/v) H 2 SO 4 to oxidize the mogrosides compound. The visualization of the results was performed after heating the plate on top of a dry incubator at 100 °C for 5 min. , The MG conversion profile of S. cerevisiae BY4741 WT served as a control, following the identical cell cultivation and analysis procedures mentioned above.…”

“…However, methods including acid hydrolysis, enzymatic treatment, and in vivo digestion all have shown little effect on generating S I from MG V due to low specificity, as the chemical structures of these two compounds only differ by one glucose moiety, where MG V has one additional glucose on the C-3 position of S I, with a β (1 → 6) linkage (Figure A). ,, Recently, the transformation of MG V using microbes has shed light on the specific production of S I from MG V. Saccharomyces cerevisiae BY4741 wild-type cells were able to produce β-glucosidase ( Sc Exg1), which acts on peripheral glucose with (1 → 6)-β- d -glucosyl linkages at the C-3 and C-24 positions of MG V, generating S I and MG IV as intermediates and MG III E as the end product . Additionally, the β-glucosidase produced by Dekkera bruxellensis ( Db Exg1) alternatively acts only on the peripheral glucose of MG V with (1 → 6)-β- d -glucosyl linkages at the C-3 position, generating only S I from MG V . To the best of our knowledge, to date, no studies have focused on using Db Exg1 in the redistribution of mogrosides for S I enrichment.…”