DEKOLORISASI LIMBAH BATIK MENGGUNAKAN LIMBAH MEDIUM TANAM Pleurotus ostreatus PADA WAKTU INKUBASI YANG BERBEDA

Abstract

The batik waste industry is one source of water pollution. It must undergo treatment before discharge into the environment. Batik waste treatment technologies can be done biologically, chemically, physically, or combination of three processes. This research applied a combination of biology and physic technologies, namely used spent mushroom of Pleurotus ostreatus as the decolorization agent. Mycelium of P. ostreatus was used as biological agent, and cellulose from spent mushroom was used as physical agent. The aims of this research were to determine the effect of spent mushroom with variation incubation time on batik waste decolorization, and to know the optimum incubation time with highest decolorization batik waste percentage using spent mushroom of P. ostreatus. This research used experimental method with completely randomized design (CRD). The data obtained were statistically analyzed using Varian Test (ANOVA) on confidence level of 95% and 99% and continued with Honest Significant Difference Test (BNJ) with 95% confidence level. The result showed that variation incubation time treatment of batik waste decolorization used spent mushroom of P. ostreatus provide a highly signification effect on batik waste decolorization. The optimum incubation time was 60 hour (LW5), with decolorization percentage was 85,64%.

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“…Adsorpsi oleh jamur terjadi ketika substansi molekul pewarna meninggalkan limbah dan bergabung pada permukaan miselium (Dewi & Lestari, 2010), sehingga terjadi perubahan warna miselium yang semula putih menjadi berwarna sesuai warna limbah yang digunakan (Dewi et al, 2016). Perubahan warna miselium jamur dari warna awal menunjukkan proses dekolorisasi yang disebabkan oleh mekanisme adsorbsi zat warna oleh miselium (Wulandari et al, 2014).…”

“…Mekanisme dekolorisasi oleh jamur secara enzimatis melibatkan enzim ekstraseluler kompleks yang disekresikan ke dalam medium kultivasi (Wulandari et al, 2014). Jamur memiliki kemampuan dalam merombak zat warna karena mampu mengekskresikan enzim ligninolitik yaitu laccase, mangan peroksidase (MnP) dan lignin peroksidase (LiP).…”

“…Aktivitas jamur dalam mendegradasi zat warna dipengaruhi oleh beberapa faktor diantaranya konsentrasi zat warna, pH, waktu inkubasi (Sukarta & Sumanhandriyani, 2013). Lama waktu inkubasi berbanding lurus dengan waktu kontak antara miselium dengan zat warna, sehingga peluang zat warna terserap lebih besar (Wulandari et al, 2014), namun penyerapan akan berkurang setelah mencapai titik jenuh penyerapan sehingga jamur akan melepaskan kembali zat warna yang terserap (Dewi & Dwiputranto, 2012).…”

Aplikasi Kitosan dari Litopenaeus vannamei dan Limbah Baglog dari Pleurotus ostreatus untuk Mendekolorisasi Limbah Cair Batik

“…This is feasible because baglog waste retains mushroom mycelium and cellulose, which can effectively adsorb dye substances present in batik wastewater (Dewi et al, 2023). Baglog waste from P. ostreatus is one of the effective materials for decolorizing batik wastewater, achieving a decolorization rate of 85.64% over 60 hours (Wulandari et al, 2014).…”

Short Communication: The higher laccase enzyme producer, Cerrena sp. BMd.TA.1, isolated from Gunung Rinjani National Park, West Nusa Tenggara, Indonesia

“…One of biotechnology use of microbes, particularly the white rot fungi (WRF), is their ligninolytic enzymes. These enzymes play significant role in mineralizing polycyclic aromatic, polychlorinated, petroleum hydrocarbon, phenolic, and dyestuffs, as well as degrading whole biomass (lignin, cellulose, and hemicellulose) naturally (Wulandari et al 2014;Suharyanto et al 2012;Yanto et al 2017;Rodríguez-Couto 2017). Other applications in biotechnology are for food, biosensor, biopulping, biobleaching, and biofuel (Septiningrum and Pramuaji 2017;Järvinen et al 2012;Rodríguez-Couto 2017).…”