Delayed luminescence of erythrosine in biological tissue and photodynamic therapy dosimetry

Letuta, S. N.; Pashkevich, S. N.; Ишемгулов, А. Т.; Lantukh, Yu. D.; Alidzhanov, E. K.; Sokabaeva, S.S.; Bryukhanov, V. V.

doi:10.1016/j.jphotobiol.2016.08.036

Cited by 20 publications

(4 citation statements)

References 28 publications

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“…The order of the delay times was kept similar with the addition of rotenone but changed slightly with AMA. Because many reports have shown that PBM-induced biological responses are mediated by intracellular ROS 34 , 36 , 38 , 39 , we examined the effects of NAC on DL. Figure 3D shows that DL after CW-PBM treatment was almost the same with and without NAC, but delay times after PW-PBM became faster at all frequencies.…”

Section: Resultsmentioning

confidence: 99%

Pulse frequency dependency of photobiomodulation on the bioenergetic functions of human dental pulp stem cells

Kim

Baik

Choung

et al. 2017

Sci Rep

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Photobiomodulation (PBM) therapy contributes to pain relief, wound healing, and tissue regeneration. The pulsed wave (PW) mode has been reported to be more effective than the continuous wave (CW) mode when applying PBM to many biological systems. However, the reason for the higher effectiveness of PW-PBM is poorly understood. Herein, we suggest using delayed luminescence (DL) as a reporter of mitochondrial activity after PBM treatment. DL originates mainly from mitochondrial electron transport chain systems, which produce reactive oxygen species (ROS) and adenosine triphosphate (ATP). The decay time of DL depends on the pulse frequencies of applied light, which correlate with the biological responses of human dental pulp stem cells (hDPSCs). Using a low-power light whose wavelength is 810 nm and energy density is 38 mJ/cm2, we find that a 300-Hz pulse frequency prolonged the DL pattern and enhanced alkaline phosphatase activity. In addition, we analyze mitochondrial morphological changes and their volume density and find evidence supporting mitochondrial physiological changes from PBM treatment. Our data suggest a new methodology for determining the effectiveness of PBM and the specific pulse frequency dependency of PBM in the differentiation of hDPSCs.

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Section: Resultsmentioning

confidence: 99%

Pulse frequency dependency of photobiomodulation on the bioenergetic functions of human dental pulp stem cells

Kim

Baik

Choung

et al. 2017

Sci Rep

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“…Практически весь наблюдаемый сигнал ЗФ красителей в живых тканях формируется только из двух компонент: ТЗФ и АЗФ [31,32]. Генерация АЗФдиффузионно-контролируемый процесс, сильно зависящий от плотности T 1 -состояний ФС и времени жизни 1 наблюдаемую ЗФ различных процессов, которые протекают с разной скоростью.…”

Section: результаты и обсуждениеunclassified

“…Особенности АЗФ различных ФС достаточно подробно изучены для небиологических систем [25][26][27][28][29], однако в живых клетках и тканях такой тип длительной люминесценции исследован очень фрагментарно [30][31][32]. Использование АЗФ имеет ряд преимуществ.…”

Section: Introductionunclassified

Мониторинг Изменения Содержания Кислорода В Тканях По Кинетике Замедленной Флуоресценции Экзогенных Красителей

Летута¹,

Пашкевич²,

Алиджанов³

et al. 2019

Журнал Технической Физики

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The dynamics of oxygen consumption during photodynamic processes and its subsequent restoration in malignant tumors and healthy tissues of mice were studied in vitro by kinetics of long-term luminescence of xanthene dyes. It was shown that to estimate changes in tissue oxygen tension, specific type of delayed fluorescence can be used which caused by singlet-triplet annihilation of singlet oxygen and a sensitizer in triplet state. In tumors under pulse excitation of sensitizers, reversible quenching of delayed fluorescence was observed, which is associated with a decrease in the tissue oxygen tension during photodynamic processes. Method for visualizing the restoration of the initial oxygen level in tissues after photodynamic action is proposed.

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“…Because of its hydrophilicity (20,21) and its important use in photodynamic therapy (22)(23)(24)(25)(26), studies involving EB are often conducted in aqueous environments. However, the term aqueous environment is often loosely defined.…”

Section: Introductionmentioning

confidence: 99%

Photobleaching of Erythrosine B in Aqueous Environment Investigation Beyond pH^†

Kravchenko

Sutherland

Heyne

2021

Photochem & Photobiology

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In the scientific literature, the term aqueous environment is loosely employed as it encompasses a broad range of different buffering agents. While there is an increasing number of experimental evidence that point toward specific buffer effects extending far beyond pH, the impact of the chemical nature of the buffering ions is often disregarded, especially in photochemical studies. Herein, we highlighted the importance of buffer specific effects on both the photobleaching and the singlet oxygen quantum yields of a dye in aqueous environments. For this study, we chose erythrosine B (EB) as our model photosensitizer as its photochemistry and photobleaching are well documented in the literature. We followed EB's photobleaching via absorption spectroscopy in four different aqueous solvents, including pure water, phosphate, Tris and HEPES buffer. These buffer systems were selected because they are commonly used in biochemical and biological applications. Our results show that specific buffer effects cannot be neglected. Indeed, the singlet oxygen quantum yield for EB is significantly different in HEPES compared to the other solvents. Furthermore, we showed that EB's photoproduct is highly dependent on the nature of the chemical buffer being used.

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Delayed luminescence of erythrosine in biological tissue and photodynamic therapy dosimetry

Cited by 20 publications

References 28 publications

Pulse frequency dependency of photobiomodulation on the bioenergetic functions of human dental pulp stem cells

Pulse frequency dependency of photobiomodulation on the bioenergetic functions of human dental pulp stem cells

Мониторинг Изменения Содержания Кислорода В Тканях По Кинетике Замедленной Флуоресценции Экзогенных Красителей

Photobleaching of Erythrosine B in Aqueous Environment Investigation Beyond pH^†

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Delayed luminescence of erythrosine in biological tissue and photodynamic therapy dosimetry

Cited by 20 publications

References 28 publications

Pulse frequency dependency of photobiomodulation on the bioenergetic functions of human dental pulp stem cells

Pulse frequency dependency of photobiomodulation on the bioenergetic functions of human dental pulp stem cells

Мониторинг Изменения Содержания Кислорода В Тканях По Кинетике Замедленной Флуоресценции Экзогенных Красителей

Photobleaching of Erythrosine B in Aqueous Environment Investigation Beyond pH†

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Photobleaching of Erythrosine B in Aqueous Environment Investigation Beyond pH^†