Delivering copper within plant cells

Himelblau, Edward; Amasino, Richard M.

doi:10.1016/s1369-5266(00)80066-7

Cited by 73 publications

(67 citation statements)

References 39 publications

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“…This gene was also induced by wilting, UV, and harpin (Table II), and its expression up-regulated in senescent leaves (data not shown). Genes encoding blue copper-binding proteins have been shown previously to respond to abiotic stresses such as drought and ozone (Cho, 1997;Langebartels et al, 2000), and expression increased during senescence, in which copper sequestration is an important event (Himelbau and Amasino, 2000). A regulatory role for ROS such as H 2 O 2 has been implicated during senescence (Pastori and del Rio, 1997), and we have already shown that H 2 O 2 induces the expression of a senescence-related gene (Desikan et al, 2000).…”

Section: Discussionmentioning

confidence: 56%

“…Among the genes induced by H 2 O 2 was one encoding a blue copper-binding protein. Such proteins might function to sequester copper, a potentially toxic element that is also an essential cellular catalyst for redox reactions (Himelbau and Amasino, 2000). This gene was also induced by wilting, UV, and harpin (Table II), and its expression up-regulated in senescent leaves (data not shown).…”

Section: Discussionmentioning

confidence: 95%

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Regulation of the Arabidopsis Transcriptome by Oxidative Stress

Mackerness²,

et al. 2001

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Oxidative stress, resulting from an imbalance in the accumulation and removal of reactive oxygen species such as hydrogen peroxide (H2O2), is a challenge faced by all aerobic organisms. In plants, exposure to various abiotic and biotic stresses results in accumulation of H2O2 and oxidative stress. Increasing evidence indicates that H2O2functions as a stress signal in plants, mediating adaptive responses to various stresses. To analyze cellular responses to H2O2, we have undertaken a large-scale analysis of the Arabidopsis transcriptome during oxidative stress. Using cDNA microarray technology, we identified 175 non-redundant expressed sequence tags that are regulated by H2O2. Of these, 113 are induced and 62 are repressed by H2O2. A substantial proportion of these expressed sequence tags have predicted functions in cell rescue and defense processes. RNA-blot analyses of selected genes were used to verify the microarray data and extend them to demonstrate that other stresses such as wilting, UV irradiation, and elicitor challenge also induce the expression of many of these genes, both independently of, and, in some cases, via H2O2.

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 95%

Regulation of the Arabidopsis Transcriptome by Oxidative Stress

Mackerness²,

et al. 2001

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“…Therefore, it is possible that copper is needed for both ethylene binding and functional receptors. RAN1 encodes a protein similar to the copper-transporting CPx class of P-type ATPases such as Ccc2 protein from Saccharomyces cerevisiae and human Menkes/Wilson proteins (31)(32)(33)(34)(35)(36). One function of these ATPases is to deliver copper to copper-containing enzymes in extracytoplasmic compartments.…”

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confidence: 99%

The Copper Transporter RAN1 Is Essential for Biogenesis of Ethylene Receptors in Arabidopsis

Binder

Rodrı́guez

Bleecker

2010

Journal of Biological Chemistry

120

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Plants utilize ethylene as a hormone to regulate multiple developmental processes and to coordinate responses to biotic and abiotic stress. In Arabidopsis thaliana, a small family of five receptor proteins typified by ETR1 mediates ethylene perception. Our previous work suggested that copper ions likely play a role in ethylene binding. An independent study indicated that the ran1 mutants, which display ethylene-like responses to the ethylene antagonist trans-cyclooctene, have mutations in the RAN1 copper-transporting P-type ATPase, once again linking copper ions to the ethylene-response pathway. The results presented herein indicate that genetically engineered Saccharomyces cerevisiae expressing ETR1 but lacking the RAN1 homolog Ccc2p (⌬ccc2) lacks ethylene-binding activity. Ethylene-binding activity was restored when copper ions were added to the ⌬ccc2 mutants, showing that it is the delivery of copper that is important. Additionally, transformation of the ⌬ccc2 mutant yeast with RAN1 rescued ethylene-binding activity. Analysis of plants carrying loss-of-function mutations in ran1 showed that they lacked ethylene-binding activity, whereas seedlings carrying weak alleles of ran1 had normal ethylene-binding activity but were hypersensitive to copper-chelating agents. Altogether, the results show an essential role for RAN1 in the biogenesis of the ethylene receptors and copper homeostasis in Arabidopsis seedlings. Furthermore, the results indicate cross-talk between the ethylene-response pathway and copper homeostasis in Arabidopsis seedling development.

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“…Since it is essential for seaweed metabolism, there might possibly exist specific transporters for this metal. In Arabidopsis thaliana, five members of a family of Cu transporters (COPT1-5) have been described and, in Saccharomyces cerevisiae, a gene (CTR1) that codifies proteins of the plasmatic membrane that transports Cu into the cell has been characterized (Dancis et al, 1994;Grotz, 1998;Himelblau & Amasino, 2000;Sancenon et al, 2003;Markossian & Kurganov, 2003). Basal natural concentrations of Cu were present in the control seaweeds (with and without Cd).…”

Section: Discussionmentioning

confidence: 99%

Absorption of metals and characterization of chemical elements present in three species of Gracilaria (Gracilariaceae) Greville: a genus of economical importance

Tonon

Oliveira

Soriano

et al. 2011

Rev. bras. farmacogn.

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Gracilaria Greville is a genus of seaweed that is economically explored by the cosmetic, pharmaceutical and food industries. One of the biggest problems associated with growing Gracilaria is the discharge of heavy metals into the marine environment. The absorption of heavy metals was investigated with the macroalga Gracilaria tenuistipitata Zhang et Xia, cultivated in a medium containing copper (Cu) and cadmium (Cd). In biological samples, EC50 concentrations of 1 ppm for cadmium and 0.95 ppm for copper were used. These concentrations were based on seaweed growth curves obtained over a period of six days in previous studies. ICP-AES was used to determine the amount of metal that seaweeds absorbed during this period. G. tenuistipitata was able to bioaccumulate both metals, about 17% of copper and 9% of cadmium. Basal natural levels of Cu were found in control seaweeds and in G. tenuistipitata exposed to Cd. In addition, the repertoire of other important chemical elements, as well as their concentrations, was determined for G. tenuistipitata and two other important seaweeds, G. birdiae Plastino & Oliveira and G. domingensis (Kützing) Sonder ex Dickie, collected in natural environments on the Brazilian shore.

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Delivering copper within plant cells

Cited by 73 publications

References 39 publications

Regulation of the Arabidopsis Transcriptome by Oxidative Stress

Regulation of the Arabidopsis Transcriptome by Oxidative Stress

The Copper Transporter RAN1 Is Essential for Biogenesis of Ethylene Receptors in Arabidopsis

Absorption of metals and characterization of chemical elements present in three species of Gracilaria (Gracilariaceae) Greville: a genus of economical importance

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